Protein insertion into the endoplasmic reticulum of permeabilized cells

M. Jadot; M. W. Hofmann; R. Graf; H. Quader; B. Martoglio

doi:10.1016/0014-5793(95)00881-9

Protein insertion into the endoplasmic reticulum of permeabilized cells

M. Jadot, M. W. Hofmann, R. Graf, H. Quader, B. Martoglio

URPHYM

Research output: Contribution to journal › Article › peer-review

Abstract

We have established efficient translocation of newly synthesized proteins into the endoplasmic reticulum of permeabilized Mel Juso cells. By site-specific photo-crosslinking me show that translocating polypeptide chains contact the same components of permeabilized cells ER as in dog pancreas rough microsomes. This cellular assay system has the potential to overcome the limitations of isolated microsomes in investigating the molecular environment of a newly synthesized protein after they have left the ER translocation site.

Original language	English
Pages (from-to)	145-148
Number of pages	4
Journal	FEBS Letters
Volume	371
Issue number	2
DOIs	https://doi.org/10.1016/0014-5793(95)00881-9
Publication status	Published - 1 Jan 1995

Keywords

Cell permeabilization
Endoplasmic reticulum
Protein translocation
Site-specific photo-crosslinking

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10.1016/0014-5793(95)00881-9

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abstract = "We have established efficient translocation of newly synthesized proteins into the endoplasmic reticulum of permeabilized Mel Juso cells. By site-specific photo-crosslinking me show that translocating polypeptide chains contact the same components of permeabilized cells ER as in dog pancreas rough microsomes. This cellular assay system has the potential to overcome the limitations of isolated microsomes in investigating the molecular environment of a newly synthesized protein after they have left the ER translocation site.",

keywords = "Cell permeabilization, Endoplasmic reticulum, Protein translocation, Site-specific photo-crosslinking",

author = "M. Jadot and Hofmann, {M. W.} and R. Graf and H. Quader and B. Martoglio",

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AU - Jadot, M.

AU - Hofmann, M. W.

AU - Graf, R.

AU - Quader, H.

AU - Martoglio, B.

PY - 1995/1/1

Y1 - 1995/1/1

N2 - We have established efficient translocation of newly synthesized proteins into the endoplasmic reticulum of permeabilized Mel Juso cells. By site-specific photo-crosslinking me show that translocating polypeptide chains contact the same components of permeabilized cells ER as in dog pancreas rough microsomes. This cellular assay system has the potential to overcome the limitations of isolated microsomes in investigating the molecular environment of a newly synthesized protein after they have left the ER translocation site.

AB - We have established efficient translocation of newly synthesized proteins into the endoplasmic reticulum of permeabilized Mel Juso cells. By site-specific photo-crosslinking me show that translocating polypeptide chains contact the same components of permeabilized cells ER as in dog pancreas rough microsomes. This cellular assay system has the potential to overcome the limitations of isolated microsomes in investigating the molecular environment of a newly synthesized protein after they have left the ER translocation site.

KW - Cell permeabilization

KW - Endoplasmic reticulum

KW - Protein translocation

KW - Site-specific photo-crosslinking

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U2 - 10.1016/0014-5793(95)00881-9

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JO - FEBS Letters

JF - FEBS Letters

IS - 2

ER -

Protein insertion into the endoplasmic reticulum of permeabilized cells

Abstract

Keywords

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