TY - JOUR
T1 - Prophylactic effects of omega-3 polyunsaturated fatty acids and luteolin on airway hyperresponsiveness and inflammation in cats with experimentally-induced asthma
AU - Leemans, Jérôme
AU - Cambier, Carole
AU - Chandler, Tony
AU - Billen, Frédéric
AU - Clercx, Cécile
AU - Kirschvink, Nathalie
AU - Gustin, Pascal
N1 - Funding Information:
Jérôme Leemans is graduate student supported by FRIA, Belgium. This study was sponsored by grants from the Région Wallonne DGTRE and Bionovate Ltd. The authors are grateful to I. Habsch for her technical assistance.
PY - 2010/4
Y1 - 2010/4
N2 - The aim of this study was to assess the preventive effects of ω-3 polyunsaturated fatty acids (ω3 PUFA) and luteolin supplementation on allergen-induced inflammation in eight Ascaris suum (AS)-sensitised cats. Airway responsiveness (AR) tests were performed and venous blood and bronchoalveolar lavage fluid (BALF) collected before and following a single (AS-stimulated) allergen exposure, as well as at the end of a 4-week treatment period, which was followed by a second AS-challenge. The ω6/ω3 fatty acid ratio in erythrocyte membranes, BALF cytology, AR to carbachol, and BALF lipoxin A4 (LXA4), an endogenous inhibitor of inflammation, were assessed at each time point.Compared to respective unstimulated values, AS-challenged cats exhibited a significant rise in BALF eosinophil percentage and there was a trend to increased BALF total cell counts, increased AR and reduced BALF LXA4 concentrations. The significant decrease in the blood ω6/ω3 ratio seen after supplementation demonstrated that ω3 PUFA were efficiently absorbed. No changes in BALF cytology were found between untreated and treated AS-stimulated cats, but BALF LXA4 levels were significantly elevated and AR significantly decreased following supplement intake. The study suggests that ω3-luteolin supplementation may have some beneficial effects on AR through a LXA4-dependent pathway in cats with experimentally-induced asthma.
AB - The aim of this study was to assess the preventive effects of ω-3 polyunsaturated fatty acids (ω3 PUFA) and luteolin supplementation on allergen-induced inflammation in eight Ascaris suum (AS)-sensitised cats. Airway responsiveness (AR) tests were performed and venous blood and bronchoalveolar lavage fluid (BALF) collected before and following a single (AS-stimulated) allergen exposure, as well as at the end of a 4-week treatment period, which was followed by a second AS-challenge. The ω6/ω3 fatty acid ratio in erythrocyte membranes, BALF cytology, AR to carbachol, and BALF lipoxin A4 (LXA4), an endogenous inhibitor of inflammation, were assessed at each time point.Compared to respective unstimulated values, AS-challenged cats exhibited a significant rise in BALF eosinophil percentage and there was a trend to increased BALF total cell counts, increased AR and reduced BALF LXA4 concentrations. The significant decrease in the blood ω6/ω3 ratio seen after supplementation demonstrated that ω3 PUFA were efficiently absorbed. No changes in BALF cytology were found between untreated and treated AS-stimulated cats, but BALF LXA4 levels were significantly elevated and AR significantly decreased following supplement intake. The study suggests that ω3-luteolin supplementation may have some beneficial effects on AR through a LXA4-dependent pathway in cats with experimentally-induced asthma.
KW - ω3-Luteolin supplementation
KW - Feline
KW - Lower airway inflammation
UR - http://www.scopus.com/inward/record.url?scp=77949329088&partnerID=8YFLogxK
U2 - 10.1016/j.tvjl.2009.01.008
DO - 10.1016/j.tvjl.2009.01.008
M3 - Article
C2 - 19231257
AN - SCOPUS:77949329088
SN - 1090-0233
VL - 184
SP - 111
EP - 114
JO - Veterinary journal
JF - Veterinary journal
IS - 1
ER -