TY - JOUR
T1 - Properties of Omp2a-Based Supported Lipid Bilayers
T2 - Comparison with Polymeric Bioinspired Membranes
AU - Puiggalí-Jou, Anna
AU - Pawlowski, J
AU - del Valle, Luis J
AU - Michaux, Catherine
AU - Perpète, Eric
AU - Sek, S
AU - Alemán, Carlos
N1 - Funding Information:
Authors acknowledge MINECO/FEDER (MAT2015-69367-R) and the Ageǹcia de Gestió d’Ajuts Universitaris i de Recerca (2017SGR359) for financial support. S.S. is grateful for the support from National Science Centre within the project no. 2016/21/B/ST4/02122. Support for the research of C.A. was received through the prize “ICREA Academia” for excellence in research funded by the Generalitat de Catalunya. C.M. and E.A.P. are grateful to the Belgian National Fund for Scientific Research for their research associate and senior research associate positions, respectively.
Publisher Copyright:
© 2018 American Chemical Society.
Copyright:
Copyright 2018 Elsevier B.V., All rights reserved.
PY - 2018/8/31
Y1 - 2018/8/31
N2 - Omp2a β-barrel outer membrane protein has been reconstituted into supported lipid bilayers (SLBs) to compare the nanomechanical properties (elastic modulus, adhesion forces, and deformation) and functionality of the resulting bioinspired system with those of Omp2a-based polymeric nanomembranes (NMs). Protein reconstitution into lipid bilayers has been performed using different strategies, the most successful one consisting of a detergent-mediated process into preformed liposomes. The elastic modulus obtained for the lipid bilayer and Omp2a are ∼19 and 10.5 ± 1.7 MPa, respectively. Accordingly, the protein is softer than the lipid bilayer, whereas the latter exhibits less mechanical strength than polymeric NMs. Besides, the function of Omp2a in the SLB is similar to that observed for Omp2a-based polymeric NMs. Results open the door to hybrid bioinspired substrates based on the integration of Omp2a-proteoliposomes and nanoperforated polymeric freestanding NMs.
AB - Omp2a β-barrel outer membrane protein has been reconstituted into supported lipid bilayers (SLBs) to compare the nanomechanical properties (elastic modulus, adhesion forces, and deformation) and functionality of the resulting bioinspired system with those of Omp2a-based polymeric nanomembranes (NMs). Protein reconstitution into lipid bilayers has been performed using different strategies, the most successful one consisting of a detergent-mediated process into preformed liposomes. The elastic modulus obtained for the lipid bilayer and Omp2a are ∼19 and 10.5 ± 1.7 MPa, respectively. Accordingly, the protein is softer than the lipid bilayer, whereas the latter exhibits less mechanical strength than polymeric NMs. Besides, the function of Omp2a in the SLB is similar to that observed for Omp2a-based polymeric NMs. Results open the door to hybrid bioinspired substrates based on the integration of Omp2a-proteoliposomes and nanoperforated polymeric freestanding NMs.
UR - http://www.scopus.com/inward/record.url?scp=85051503879&partnerID=8YFLogxK
U2 - 10.1021/acsomega.8b00913
DO - 10.1021/acsomega.8b00913
M3 - Article
VL - 3
SP - 9003
EP - 9019
JO - ACS Omega
JF - ACS Omega
IS - 8
ER -