Probing ligand-protein recognition with sum-frequency generation spectroscopy: the avidin-biocytin case

Laurent Dreesen; Yannick Sartenaer; Christophe Humbert; Alaa Addin Mani; Christophe Méthivier; Claire-Marie Pradier; Paul Thiry; André Peremans

Probing ligand-protein recognition with sum-frequency generation spectroscopy: the avidin-biocytin case

Laurent Dreesen, Yannick Sartenaer, Christophe Humbert, Alaa Addin Mani, Christophe Méthivier, Claire-Marie Pradier, Paul Thiry, André Peremans

Namur Research Institute for Life Sciences

Research output: Contribution to journal › Article

Abstract

Infrared/visible sum-frequency generation (SFG) spectroscopy is used to study the recognition of a protein (avidin) by a derived vitamin (biocytin) adsorbed on a calcium fluoride substrate. The specificity of the process is tested by replacing avidin with bovine serum albumin or presaturated avidin. The SFG spectroscopy shows drastic modifications in the CH and NH spectral ranges only upon exposure of the biocytin film to avidin. The comparison of the SFG data with Fourier transform infrared reflection absorption spectra (FT-IRRAS) in the same spectral ranges illustrates the advantages of nonlinear spectroscopy for studying and detecting recognition between biomolecules.

Original language	English
Pages (from-to)	1719-1725
Number of pages	7
Journal	ChemPhysChem
Volume	5
Issue number	11
Publication status	Published - 2004

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http://dx.doi.org/10.1002/cphc.2004

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title = "Probing ligand-protein recognition with sum-frequency generation spectroscopy: the avidin-biocytin case",

abstract = "Infrared/visible sum-frequency generation (SFG) spectroscopy is used to study the recognition of a protein (avidin) by a derived vitamin (biocytin) adsorbed on a calcium fluoride substrate. The specificity of the process is tested by replacing avidin with bovine serum albumin or presaturated avidin. The SFG spectroscopy shows drastic modifications in the CH and NH spectral ranges only upon exposure of the biocytin film to avidin. The comparison of the SFG data with Fourier transform infrared reflection absorption spectra (FT-IRRAS) in the same spectral ranges illustrates the advantages of nonlinear spectroscopy for studying and detecting recognition between biomolecules.",

author = "Laurent Dreesen and Yannick Sartenaer and Christophe Humbert and Mani, {Alaa Addin} and Christophe M{\'e}thivier and Claire-Marie Pradier and Paul Thiry and Andr{\'e} Peremans",

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T1 - Probing ligand-protein recognition with sum-frequency generation spectroscopy: the avidin-biocytin case

AU - Dreesen, Laurent

AU - Sartenaer, Yannick

AU - Humbert, Christophe

AU - Mani, Alaa Addin

AU - Méthivier, Christophe

AU - Pradier, Claire-Marie

AU - Thiry, Paul

AU - Peremans, André

PY - 2004

Y1 - 2004

N2 - Infrared/visible sum-frequency generation (SFG) spectroscopy is used to study the recognition of a protein (avidin) by a derived vitamin (biocytin) adsorbed on a calcium fluoride substrate. The specificity of the process is tested by replacing avidin with bovine serum albumin or presaturated avidin. The SFG spectroscopy shows drastic modifications in the CH and NH spectral ranges only upon exposure of the biocytin film to avidin. The comparison of the SFG data with Fourier transform infrared reflection absorption spectra (FT-IRRAS) in the same spectral ranges illustrates the advantages of nonlinear spectroscopy for studying and detecting recognition between biomolecules.

AB - Infrared/visible sum-frequency generation (SFG) spectroscopy is used to study the recognition of a protein (avidin) by a derived vitamin (biocytin) adsorbed on a calcium fluoride substrate. The specificity of the process is tested by replacing avidin with bovine serum albumin or presaturated avidin. The SFG spectroscopy shows drastic modifications in the CH and NH spectral ranges only upon exposure of the biocytin film to avidin. The comparison of the SFG data with Fourier transform infrared reflection absorption spectra (FT-IRRAS) in the same spectral ranges illustrates the advantages of nonlinear spectroscopy for studying and detecting recognition between biomolecules.

M3 - Article

SN - 1439-7641

VL - 5

SP - 1719

EP - 1725

JO - ChemPhysChem

JF - ChemPhysChem

IS - 11

ER -

Probing ligand-protein recognition with sum-frequency generation spectroscopy: the avidin-biocytin case

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