Probing ligand-protein recognition with sum-frequency generation spectroscopy: the avidin-biocytin case

Laurent Dreesen, Yannick Sartenaer, Christophe Humbert, Alaa Addin Mani, Christophe Méthivier, Claire-Marie Pradier, Paul Thiry, André Peremans

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Abstract

Infrared/visible sum-frequency generation (SFG) spectroscopy is used to study the recognition of a protein (avidin) by a derived vitamin (biocytin) adsorbed on a calcium fluoride substrate. The specificity of the process is tested by replacing avidin with bovine serum albumin or presaturated avidin. The SFG spectroscopy shows drastic modifications in the CH and NH spectral ranges only upon exposure of the biocytin film to avidin. The comparison of the SFG data with Fourier transform infrared reflection absorption spectra (FT-IRRAS) in the same spectral ranges illustrates the advantages of nonlinear spectroscopy for studying and detecting recognition between biomolecules.
Original languageEnglish
Pages (from-to)1719-1725
Number of pages7
JournalChemPhysChem
Volume5
Issue number11
Publication statusPublished - 2004

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Dreesen, L., Sartenaer, Y., Humbert, C., Mani, A. A., Méthivier, C., Pradier, C-M., ... Peremans, A. (2004). Probing ligand-protein recognition with sum-frequency generation spectroscopy: the avidin-biocytin case. ChemPhysChem, 5(11), 1719-1725.