TY - JOUR
T1 - Prevalence of Capnocytophaga canimorsus in dogs and occurrence of potential virulence factors
AU - Mally, Manuela
AU - Paroz, Cécile
AU - Shin, Hwain
AU - Meyer, Salome
AU - Soussoula, Lavinia V
AU - Schmiediger, Ueli
AU - Saillen-Paroz, Caroline
AU - Cornelis, Guy R
PY - 2009
Y1 - 2009
N2 - Capnocytophaga canimorsus is a Gram-negative commensal of dog's mouth causing severe human infections. A strain isolated from a human fatal infection was recently shown to have a sialidase, to inhibit the bactericidal activity of macrophages and to block the release of nitric oxide by LPS-stimulated macrophages. The present study aimed at determining the prevalence of C. canimorsus in dogs and the occurrence of these hypothetical virulence factors. C. canimorsus could be retrieved from the saliva of 61 dogs out of 106 sampled. Like in clinical isolates, all dog strains had a sialidase and 60% blocked the killing of phagocytosed Escherichia coli by macrophages. In contrast, only 6.5% of dog strains blocked the release of nitric oxide by LPS-challenged macrophages, suggesting that this property might contribute to virulence. The comparative analysis of 69 16S rDNA sequences revealed the existence of C. canimorsus strains that could be misdiagnosed.
AB - Capnocytophaga canimorsus is a Gram-negative commensal of dog's mouth causing severe human infections. A strain isolated from a human fatal infection was recently shown to have a sialidase, to inhibit the bactericidal activity of macrophages and to block the release of nitric oxide by LPS-stimulated macrophages. The present study aimed at determining the prevalence of C. canimorsus in dogs and the occurrence of these hypothetical virulence factors. C. canimorsus could be retrieved from the saliva of 61 dogs out of 106 sampled. Like in clinical isolates, all dog strains had a sialidase and 60% blocked the killing of phagocytosed Escherichia coli by macrophages. In contrast, only 6.5% of dog strains blocked the release of nitric oxide by LPS-challenged macrophages, suggesting that this property might contribute to virulence. The comparative analysis of 69 16S rDNA sequences revealed the existence of C. canimorsus strains that could be misdiagnosed.
U2 - 10.1016/j.micinf.2009.02.005
DO - 10.1016/j.micinf.2009.02.005
M3 - Article
C2 - 19285152
SN - 1769-714X
VL - 11
SP - 509
EP - 514
JO - Microbes and infection : a journal on infectious agents and host defenses
JF - Microbes and infection : a journal on infectious agents and host defenses
IS - 4
ER -