Positive selection of recombinant plasmids based on the EcoK restriction activity of Escherichia coli K-12

O De Backer; P Chomez; E De Plaen

Positive selection of recombinant plasmids based on the EcoK restriction activity of Escherichia coli K-12

O De Backer, P Chomez, E De Plaen

Namur Research Institute for Life Sciences

Research output: Contribution to journal › Article › peer-review

Abstract

We have constructed a pTZ19R-derived vector which allows efficient positive selection of recombinant plasmids. The system uses the EcoK restriction activity of Escherichia coli K-12 to select against non-recombinant plasmids. The vector contains an EcoK site which, if deleted or disrupted by ligating a DNA fragment, yields recombinant plasmids that are no longer susceptible to EcoK restriction when transformed into a restriction-proficient E. coli host.

Original language	English
Pages (from-to)	197-8
Number of pages	2
Journal	Gene
Volume	150
Issue number	1
Publication status	Published - 1994

Cite this

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title = "Positive selection of recombinant plasmids based on the EcoK restriction activity of Escherichia coli K-12",

abstract = "We have constructed a pTZ19R-derived vector which allows efficient positive selection of recombinant plasmids. The system uses the EcoK restriction activity of Escherichia coli K-12 to select against non-recombinant plasmids. The vector contains an EcoK site which, if deleted or disrupted by ligating a DNA fragment, yields recombinant plasmids that are no longer susceptible to EcoK restriction when transformed into a restriction-proficient E. coli host.",

author = "{De Backer}, O and P Chomez and {De Plaen}, E",

year = "1994",

language = "English",

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journal = "Gene",

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T1 - Positive selection of recombinant plasmids based on the EcoK restriction activity of Escherichia coli K-12

AU - De Backer, O

AU - Chomez, P

AU - De Plaen, E

PY - 1994

Y1 - 1994

N2 - We have constructed a pTZ19R-derived vector which allows efficient positive selection of recombinant plasmids. The system uses the EcoK restriction activity of Escherichia coli K-12 to select against non-recombinant plasmids. The vector contains an EcoK site which, if deleted or disrupted by ligating a DNA fragment, yields recombinant plasmids that are no longer susceptible to EcoK restriction when transformed into a restriction-proficient E. coli host.

AB - We have constructed a pTZ19R-derived vector which allows efficient positive selection of recombinant plasmids. The system uses the EcoK restriction activity of Escherichia coli K-12 to select against non-recombinant plasmids. The vector contains an EcoK site which, if deleted or disrupted by ligating a DNA fragment, yields recombinant plasmids that are no longer susceptible to EcoK restriction when transformed into a restriction-proficient E. coli host.

M3 - Article

C2 - 7959053

SN - 0378-1119

VL - 150

SP - 197

EP - 198

JO - Gene

JF - Gene

IS - 1

ER -

Positive selection of recombinant plasmids based on the EcoK restriction activity of Escherichia coli K-12

Abstract

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