Polypyrrole-supported membrane proteins for bio-inspired ion channels

Maria M. Pérez-Madrigal, Luis J. Del Valle, Elaine Armelin, Catherine Michaux, Guillaume Roussel, Eric A. Perpète, Carlos Alemán

Research output: Contribution to journalArticle

Abstract

Biomedical platforms constructed by immobilizing membrane proteins in matrixes made of synthetic organic polymers is a challenge because the structure and function of these proteins are affected by environmental conditions. In this work, an operative composite that regulates the diffusion of alkali ions has been prepared by functionalizing a supporting matrix made of poly(N-methylpyrrole) (PNMPy) with a β-barrel membrane protein (Omp2a) that forms channels and pores. The protein has been unequivocally identified in the composite, and its structure has been shown to remain unaltered. The PNMPy-Omp2a platform fulfills properties typically associated with functional bio-interfaces with biomedical applications (e.g., biocompatibility, biodegrabadility, and hydrophilicity). The functionality of the immobilized protein has been examined by studying the passive ion transport response in the presence of electrolytic solutions with Na+ and K+ concentrations close to those found in blood. Although the behavior of PNMPy and PNMPy-Omp2a is very similar for solutions with very low concentration, the resistance of the latter decreases drastically when the concentration of ions increases to ∼100 mM. This reduction reflects an enhanced ion exchange between the biocomposite and the electrolytic medium, which is not observed in PNMPy, evidencing that PNMPy-Omp2a is particularly well suited to prepare bioinspired channels and smart biosensors.

Original languageEnglish
Pages (from-to)1632-1643
Number of pages12
JournalACS Applied Materials and Interfaces
Volume7
Issue number3
DOIs
Publication statusPublished - 2015

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Keywords

  • bioinspired channels
  • electroactive polymer
  • electrochemical impedance spectroscopy
  • functional bio-interfaces
  • ion exchange

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