Polyamines and pectins. I. Ion exchange and selectivity

Research output: Contribution to journalArticle

Abstract

The ion binding and exchange properties of putrescine, spermidine and spermine on purified walls of carrot cell suspensions were investigated by performing ion exchange isotherms, and compared to the behavior of Na+, Mg2+ and Ca2+. The cation exchange capacity of the carrot cell walls was 0.8 equivalent kg-1 dry matter and the ionic selectivity sequence of the walls for polyamines followed the sequence: spermine4+ > spermidine3+  calcium2+ > putrescine2+. The polyamines were only subjected to electroselectivity and probably did not induce any favorable supramolecular conformation of pectin like the one induced by calcium. Triangular ion exchanges were also performed with three diamines: ethane diamine, butanediamine and octanediamine. The shorter was the diamine, the higher the total adsorption and the selectivity of the exchange. The lower selectivity of the cell wall for putrescine was partly attributed to its inability to access and displace calcium from higher affinity sites within dimerized pectic sequences. The polyamine adsorption and exchange on pectic sequences could result in pectic signal modulation in pathogenesis as well as in differentiation.
Original languageEnglish
Pages (from-to)387-395
Number of pages9
JournalPlant Physiology
Volume113
Publication statusPublished - 1997

Fingerprint

Pectins
diamines
Diamines
Ion Exchange
Polyamines
ion exchange
polyamines
pectins
Cell Wall
Daucus carota
Putrescine
putrescine
calcium
carrots
Adsorption
adsorption
cell walls
Calcium
ethane
Ethane

Keywords

  • carrot
  • spermine
  • polygalacturonic acid
  • polyamines
  • ion exchange isotherm
  • pectin
  • putrescine
  • spermidine
  • Cell wall

Cite this

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title = "Polyamines and pectins. I. Ion exchange and selectivity",
abstract = "The ion binding and exchange properties of putrescine, spermidine and spermine on purified walls of carrot cell suspensions were investigated by performing ion exchange isotherms, and compared to the behavior of Na+, Mg2+ and Ca2+. The cation exchange capacity of the carrot cell walls was 0.8 equivalent kg-1 dry matter and the ionic selectivity sequence of the walls for polyamines followed the sequence: spermine4+ > spermidine3+  calcium2+ > putrescine2+. The polyamines were only subjected to electroselectivity and probably did not induce any favorable supramolecular conformation of pectin like the one induced by calcium. Triangular ion exchanges were also performed with three diamines: ethane diamine, butanediamine and octanediamine. The shorter was the diamine, the higher the total adsorption and the selectivity of the exchange. The lower selectivity of the cell wall for putrescine was partly attributed to its inability to access and displace calcium from higher affinity sites within dimerized pectic sequences. The polyamine adsorption and exchange on pectic sequences could result in pectic signal modulation in pathogenesis as well as in differentiation.",
keywords = "carrot , spermine, polygalacturonic acid, polyamines, ion exchange isotherm, pectin, putrescine, spermidine, Cell wall",
author = "Johan Messiaen and Pierre Cambier and {Van Cutsem}, Pierre",
year = "1997",
language = "English",
volume = "113",
pages = "387--395",
journal = "Plant Physiology",
issn = "0032-0889",
publisher = "American Society of Plant Biologists",

}

Polyamines and pectins. I. Ion exchange and selectivity. / Messiaen, Johan; Cambier, Pierre; Van Cutsem, Pierre.

In: Plant Physiology, Vol. 113, 1997, p. 387-395.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Polyamines and pectins. I. Ion exchange and selectivity

AU - Messiaen, Johan

AU - Cambier, Pierre

AU - Van Cutsem, Pierre

PY - 1997

Y1 - 1997

N2 - The ion binding and exchange properties of putrescine, spermidine and spermine on purified walls of carrot cell suspensions were investigated by performing ion exchange isotherms, and compared to the behavior of Na+, Mg2+ and Ca2+. The cation exchange capacity of the carrot cell walls was 0.8 equivalent kg-1 dry matter and the ionic selectivity sequence of the walls for polyamines followed the sequence: spermine4+ > spermidine3+  calcium2+ > putrescine2+. The polyamines were only subjected to electroselectivity and probably did not induce any favorable supramolecular conformation of pectin like the one induced by calcium. Triangular ion exchanges were also performed with three diamines: ethane diamine, butanediamine and octanediamine. The shorter was the diamine, the higher the total adsorption and the selectivity of the exchange. The lower selectivity of the cell wall for putrescine was partly attributed to its inability to access and displace calcium from higher affinity sites within dimerized pectic sequences. The polyamine adsorption and exchange on pectic sequences could result in pectic signal modulation in pathogenesis as well as in differentiation.

AB - The ion binding and exchange properties of putrescine, spermidine and spermine on purified walls of carrot cell suspensions were investigated by performing ion exchange isotherms, and compared to the behavior of Na+, Mg2+ and Ca2+. The cation exchange capacity of the carrot cell walls was 0.8 equivalent kg-1 dry matter and the ionic selectivity sequence of the walls for polyamines followed the sequence: spermine4+ > spermidine3+  calcium2+ > putrescine2+. The polyamines were only subjected to electroselectivity and probably did not induce any favorable supramolecular conformation of pectin like the one induced by calcium. Triangular ion exchanges were also performed with three diamines: ethane diamine, butanediamine and octanediamine. The shorter was the diamine, the higher the total adsorption and the selectivity of the exchange. The lower selectivity of the cell wall for putrescine was partly attributed to its inability to access and displace calcium from higher affinity sites within dimerized pectic sequences. The polyamine adsorption and exchange on pectic sequences could result in pectic signal modulation in pathogenesis as well as in differentiation.

KW - carrot

KW - spermine

KW - polygalacturonic acid

KW - polyamines

KW - ion exchange isotherm

KW - pectin

KW - putrescine

KW - spermidine

KW - Cell wall

M3 - Article

VL - 113

SP - 387

EP - 395

JO - Plant Physiology

JF - Plant Physiology

SN - 0032-0889

ER -