Platelet microparticle generation assay: A valuable test for immune heparin-induced thrombocytopenia diagnosis

F. Mullier, V. Minet, N. Bailly, B. Devalet, J. Douxfils, C. Chatelain, I. Elalamy, J.M. Dogné, B. Chatelain

Research output: Contribution to journalArticle

208 Downloads (Pure)

Abstract

Background: Early diagnosis of immune heparin-induced thrombocytopenia (HIT) is essential to improve clinical outcome but remains challenging. The release of platelet microparticles (PMPs) is considered of major pathophysiological significance. Objectives: The aim of this study was to evaluate performances of PMP generation assay (PMPGA) compared to clinical outcome to diagnose HIT. The second objective was to compare PMPGA with performances of C-serotonin release assay (SRA) on the same series of patients. Methods: Sera of 53 HIT-suspected patients were retrospectively incubated with citrated-whole blood from healthy donors with 1 IU and 500 IU/ml of unfractionated heparin (UH). PMPGA was performed using FACSAria® flow cytometer. The clinical diagnosis was established by two blinded independent investigators analysing in a standardized manner the patient's medical records. Performances of PMPGA and SRA (n = 53) were evaluated using ROC curve analysis with clinical outcome as reference. Results: In positive HIT patients, PMPs expressing phosphatidylserine are generated with low UH concentration whereas PMP rate decreases significantly in presence of high UH concentration. Using clinical outcome as reference, sensitivity and specificity of PMPGA reached 88.9% (95% CI: 50.7-99.4) and 100.0% (95% CI: 90.0-100.0). Sensitivity and specificity of C-SRA were 88.9% (95% CI: 50.7-99.4) and 95.5% (95% CI: 83.3-99.2). Conclusions: PMPGA is a rapid and reliable assay for HIT diagnosis. PMPGA showed good correlation with C-SRA performances and predominately with clinical outcome.
Original languageEnglish
Pages (from-to)1068-1073
Number of pages6
JournalThrombosis Research
Volume133
Issue number6
DOIs
Publication statusPublished - 30 Jun 2014

Fingerprint

Thrombocytopenia
Heparin
Blood Platelets
Serotonin
ROC Curve
Sensitivity and Specificity
Phosphatidylserines
Blood Donors
Medical Records
Early Diagnosis
Research Personnel
Serum

Keywords

  • heparin-induced thrombocytopenia
  • microparticles
  • platelets
  • thrombosis

Cite this

@article{966fdcbfae4b41d1ab5851c4467713b8,
title = "Platelet microparticle generation assay: A valuable test for immune heparin-induced thrombocytopenia diagnosis",
abstract = "Background: Early diagnosis of immune heparin-induced thrombocytopenia (HIT) is essential to improve clinical outcome but remains challenging. The release of platelet microparticles (PMPs) is considered of major pathophysiological significance. Objectives: The aim of this study was to evaluate performances of PMP generation assay (PMPGA) compared to clinical outcome to diagnose HIT. The second objective was to compare PMPGA with performances of C-serotonin release assay (SRA) on the same series of patients. Methods: Sera of 53 HIT-suspected patients were retrospectively incubated with citrated-whole blood from healthy donors with 1 IU and 500 IU/ml of unfractionated heparin (UH). PMPGA was performed using FACSAria{\circledR} flow cytometer. The clinical diagnosis was established by two blinded independent investigators analysing in a standardized manner the patient's medical records. Performances of PMPGA and SRA (n = 53) were evaluated using ROC curve analysis with clinical outcome as reference. Results: In positive HIT patients, PMPs expressing phosphatidylserine are generated with low UH concentration whereas PMP rate decreases significantly in presence of high UH concentration. Using clinical outcome as reference, sensitivity and specificity of PMPGA reached 88.9{\%} (95{\%} CI: 50.7-99.4) and 100.0{\%} (95{\%} CI: 90.0-100.0). Sensitivity and specificity of C-SRA were 88.9{\%} (95{\%} CI: 50.7-99.4) and 95.5{\%} (95{\%} CI: 83.3-99.2). Conclusions: PMPGA is a rapid and reliable assay for HIT diagnosis. PMPGA showed good correlation with C-SRA performances and predominately with clinical outcome.",
keywords = "heparin-induced thrombocytopenia, microparticles, platelets, thrombosis",
author = "F. Mullier and V. Minet and N. Bailly and B. Devalet and J. Douxfils and C. Chatelain and I. Elalamy and J.M. Dogn{\'e} and B. Chatelain",
year = "2014",
month = "6",
day = "30",
doi = "10.1016/j.thromres.2013.12.009",
language = "English",
volume = "133",
pages = "1068--1073",
journal = "Thrombosis Research",
issn = "0049-3848",
publisher = "Elsevier",
number = "6",

}

Platelet microparticle generation assay : A valuable test for immune heparin-induced thrombocytopenia diagnosis. / Mullier, F.; Minet, V.; Bailly, N.; Devalet, B.; Douxfils, J.; Chatelain, C.; Elalamy, I.; Dogné, J.M.; Chatelain, B.

In: Thrombosis Research, Vol. 133, No. 6, 30.06.2014, p. 1068-1073.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Platelet microparticle generation assay

T2 - A valuable test for immune heparin-induced thrombocytopenia diagnosis

AU - Mullier, F.

AU - Minet, V.

AU - Bailly, N.

AU - Devalet, B.

AU - Douxfils, J.

AU - Chatelain, C.

AU - Elalamy, I.

AU - Dogné, J.M.

AU - Chatelain, B.

PY - 2014/6/30

Y1 - 2014/6/30

N2 - Background: Early diagnosis of immune heparin-induced thrombocytopenia (HIT) is essential to improve clinical outcome but remains challenging. The release of platelet microparticles (PMPs) is considered of major pathophysiological significance. Objectives: The aim of this study was to evaluate performances of PMP generation assay (PMPGA) compared to clinical outcome to diagnose HIT. The second objective was to compare PMPGA with performances of C-serotonin release assay (SRA) on the same series of patients. Methods: Sera of 53 HIT-suspected patients were retrospectively incubated with citrated-whole blood from healthy donors with 1 IU and 500 IU/ml of unfractionated heparin (UH). PMPGA was performed using FACSAria® flow cytometer. The clinical diagnosis was established by two blinded independent investigators analysing in a standardized manner the patient's medical records. Performances of PMPGA and SRA (n = 53) were evaluated using ROC curve analysis with clinical outcome as reference. Results: In positive HIT patients, PMPs expressing phosphatidylserine are generated with low UH concentration whereas PMP rate decreases significantly in presence of high UH concentration. Using clinical outcome as reference, sensitivity and specificity of PMPGA reached 88.9% (95% CI: 50.7-99.4) and 100.0% (95% CI: 90.0-100.0). Sensitivity and specificity of C-SRA were 88.9% (95% CI: 50.7-99.4) and 95.5% (95% CI: 83.3-99.2). Conclusions: PMPGA is a rapid and reliable assay for HIT diagnosis. PMPGA showed good correlation with C-SRA performances and predominately with clinical outcome.

AB - Background: Early diagnosis of immune heparin-induced thrombocytopenia (HIT) is essential to improve clinical outcome but remains challenging. The release of platelet microparticles (PMPs) is considered of major pathophysiological significance. Objectives: The aim of this study was to evaluate performances of PMP generation assay (PMPGA) compared to clinical outcome to diagnose HIT. The second objective was to compare PMPGA with performances of C-serotonin release assay (SRA) on the same series of patients. Methods: Sera of 53 HIT-suspected patients were retrospectively incubated with citrated-whole blood from healthy donors with 1 IU and 500 IU/ml of unfractionated heparin (UH). PMPGA was performed using FACSAria® flow cytometer. The clinical diagnosis was established by two blinded independent investigators analysing in a standardized manner the patient's medical records. Performances of PMPGA and SRA (n = 53) were evaluated using ROC curve analysis with clinical outcome as reference. Results: In positive HIT patients, PMPs expressing phosphatidylserine are generated with low UH concentration whereas PMP rate decreases significantly in presence of high UH concentration. Using clinical outcome as reference, sensitivity and specificity of PMPGA reached 88.9% (95% CI: 50.7-99.4) and 100.0% (95% CI: 90.0-100.0). Sensitivity and specificity of C-SRA were 88.9% (95% CI: 50.7-99.4) and 95.5% (95% CI: 83.3-99.2). Conclusions: PMPGA is a rapid and reliable assay for HIT diagnosis. PMPGA showed good correlation with C-SRA performances and predominately with clinical outcome.

KW - heparin-induced thrombocytopenia

KW - microparticles

KW - platelets

KW - thrombosis

UR - http://www.scopus.com/inward/record.url?scp=84900385952&partnerID=8YFLogxK

U2 - 10.1016/j.thromres.2013.12.009

DO - 10.1016/j.thromres.2013.12.009

M3 - Article

C2 - 24360929

AN - SCOPUS:84900385952

VL - 133

SP - 1068

EP - 1073

JO - Thrombosis Research

JF - Thrombosis Research

SN - 0049-3848

IS - 6

ER -