Peptide immobilization onto radiation grafted PVDF-g-poly (acrylic-acid) films surface via a spacer arm

M.Cl. Clochard; N. Betz; M. Goncalves; Carla Bittencourt Papaleo Montes; Jean-Jacques Pireaux; K. Gionnet; G. Deleris; A Le Moel

doi:10.1016/j.nimb.2005.04.029

Peptide immobilization onto radiation grafted PVDF-g-poly (acrylic-acid) films surface via a spacer arm

M.Cl. Clochard, N. Betz, M. Goncalves, Carla Bittencourt Papaleo Montes, Jean-Jacques Pireaux, K. Gionnet, G. Deleris, A Le Moel

Namur Research Institute for Life Sciences

Research output: Contribution to journal › Article

Abstract

Introducing hydrophilic functions on poly(vinylidene fluoride) (PVDF) films surface allows the covalent immobilization of peptides. Therefore radiation grafting of acrylic acid (AA) in pre-irradiated PVDF films was achieved to allow surface functionalization with linear and cyclic peptides. Peptides were bound via spacer molecules using EDC as a coupling agent. The reactions were followed by Fourier Transform Infrared (FTIR) spectroscopy in attenuated total reflection (ATR) mode. The amount of immobilized peptides was determined by UV spectroscopy. As well, an uncommon method for PVDF characterization and reactions quantification was used: high-resolution-magic angle spinning nuclear mass spectroscopy (HR-MAS NMR). Spacer saturation of the film surface corresponded to 25 mol% yield meaning that one spacer on 4 carboxylic acids was covalently bound. XPS experiments were also performed to deepen analysis of the surface composition. Peptide density is governed by steric hindrance. ELISA tests showed that the peptides’ activity is maintained.

Original language	English
Pages (from-to)	208-215
Number of pages	8
Journal	Nuclear Instruments and Methods in Physics Research, Section B: Beam Interactions with Materials and Atoms
Volume	236
Issue number	1-4
DOIs	https://doi.org/10.1016/j.nimb.2005.04.029
Publication status	Published - 2005

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10.1016/j.nimb.2005.04.029

Synthesis, Irradiation and Analysis of Materials (SIAM)
Louette, P. (Manager), Colaux, J. (Manager), Felten, A. (Manager), Tabarrant, T. (Operator), COME, F. (Operator) & Debarsy, P.-L. (Manager)
Technological Platform Synthesis, Irradiation and Analysis of Materials
Facility/equipment: Technological Platform

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Clochard, M. C., Betz, N., Goncalves, M., Bittencourt Papaleo Montes, C., Pireaux, J.-J., Gionnet, K., Deleris, G., & Le Moel, A. (2005). Peptide immobilization onto radiation grafted PVDF-g-poly (acrylic-acid) films surface via a spacer arm. Nuclear Instruments and Methods in Physics Research, Section B: Beam Interactions with Materials and Atoms, 236(1-4), 208-215. https://doi.org/10.1016/j.nimb.2005.04.029

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title = "Peptide immobilization onto radiation grafted PVDF-g-poly (acrylic-acid) films surface via a spacer arm",

abstract = "Introducing hydrophilic functions on poly(vinylidene fluoride) (PVDF) films surface allows the covalent immobilization of peptides. Therefore radiation grafting of acrylic acid (AA) in pre-irradiated PVDF films was achieved to allow surface functionalization with linear and cyclic peptides. Peptides were bound via spacer molecules using EDC as a coupling agent. The reactions were followed by Fourier Transform Infrared (FTIR) spectroscopy in attenuated total reflection (ATR) mode. The amount of immobilized peptides was determined by UV spectroscopy. As well, an uncommon method for PVDF characterization and reactions quantification was used: high-resolution-magic angle spinning nuclear mass spectroscopy (HR-MAS NMR). Spacer saturation of the film surface corresponded to 25 mol% yield meaning that one spacer on 4 carboxylic acids was covalently bound. XPS experiments were also performed to deepen analysis of the surface composition. Peptide density is governed by steric hindrance. ELISA tests showed that the peptides{\textquoteright} activity is maintained.",

author = "M.Cl. Clochard and N. Betz and M. Goncalves and {Bittencourt Papaleo Montes}, Carla and Jean-Jacques Pireaux and K. Gionnet and G. Deleris and {Le Moel}, A",

year = "2005",

doi = "10.1016/j.nimb.2005.04.029",

language = "English",

volume = "236",

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Clochard, MC, Betz, N, Goncalves, M, Bittencourt Papaleo Montes, C, Pireaux, J-J, Gionnet, K, Deleris, G & Le Moel, A 2005, 'Peptide immobilization onto radiation grafted PVDF-g-poly (acrylic-acid) films surface via a spacer arm', Nuclear Instruments and Methods in Physics Research, Section B: Beam Interactions with Materials and Atoms, vol. 236, no. 1-4, pp. 208-215. https://doi.org/10.1016/j.nimb.2005.04.029

TY - JOUR

T1 - Peptide immobilization onto radiation grafted PVDF-g-poly (acrylic-acid) films surface via a spacer arm

AU - Clochard, M.Cl.

AU - Betz, N.

AU - Goncalves, M.

AU - Bittencourt Papaleo Montes, Carla

AU - Pireaux, Jean-Jacques

AU - Gionnet, K.

AU - Deleris, G.

AU - Le Moel, A

PY - 2005

Y1 - 2005

N2 - Introducing hydrophilic functions on poly(vinylidene fluoride) (PVDF) films surface allows the covalent immobilization of peptides. Therefore radiation grafting of acrylic acid (AA) in pre-irradiated PVDF films was achieved to allow surface functionalization with linear and cyclic peptides. Peptides were bound via spacer molecules using EDC as a coupling agent. The reactions were followed by Fourier Transform Infrared (FTIR) spectroscopy in attenuated total reflection (ATR) mode. The amount of immobilized peptides was determined by UV spectroscopy. As well, an uncommon method for PVDF characterization and reactions quantification was used: high-resolution-magic angle spinning nuclear mass spectroscopy (HR-MAS NMR). Spacer saturation of the film surface corresponded to 25 mol% yield meaning that one spacer on 4 carboxylic acids was covalently bound. XPS experiments were also performed to deepen analysis of the surface composition. Peptide density is governed by steric hindrance. ELISA tests showed that the peptides’ activity is maintained.

AB - Introducing hydrophilic functions on poly(vinylidene fluoride) (PVDF) films surface allows the covalent immobilization of peptides. Therefore radiation grafting of acrylic acid (AA) in pre-irradiated PVDF films was achieved to allow surface functionalization with linear and cyclic peptides. Peptides were bound via spacer molecules using EDC as a coupling agent. The reactions were followed by Fourier Transform Infrared (FTIR) spectroscopy in attenuated total reflection (ATR) mode. The amount of immobilized peptides was determined by UV spectroscopy. As well, an uncommon method for PVDF characterization and reactions quantification was used: high-resolution-magic angle spinning nuclear mass spectroscopy (HR-MAS NMR). Spacer saturation of the film surface corresponded to 25 mol% yield meaning that one spacer on 4 carboxylic acids was covalently bound. XPS experiments were also performed to deepen analysis of the surface composition. Peptide density is governed by steric hindrance. ELISA tests showed that the peptides’ activity is maintained.

U2 - 10.1016/j.nimb.2005.04.029

DO - 10.1016/j.nimb.2005.04.029

M3 - Article

SN - 0168-583X

VL - 236

SP - 208

EP - 215

JO - Nuclear Instruments and Methods in Physics Research, Section B: Beam Interactions with Materials and Atoms

JF - Nuclear Instruments and Methods in Physics Research, Section B: Beam Interactions with Materials and Atoms

IS - 1-4

ER -

Peptide immobilization onto radiation grafted PVDF-g-poly (acrylic-acid) films surface via a spacer arm

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