PdeA is required for the rod shape morphology of Brucella abortus

Angéline Reboul, Elodie Carlier, François Xavier Stubbe, Emeline Barbieux, Aurore Demars, Phuong Thi Anh Ong, Antoine Gerodez, Eric Muraille, Xavier De Bolle

Research output: Contribution to journalArticlepeer-review

Abstract

Cyclic-di-GMP plays crucial role in the cell cycle regulation of the α-Proteobacterium Caulobacter crescentus. Here we investigated its role in the α-Proteobacterium Brucella abortus, a zoonotic intracellular pathogen. Surprisingly, deletion of all predicted cyclic-di-GMP synthesizing or degrading enzymes did not drastically impair the growth of B. abortus, nor its ability to grow inside cell lines. As other Rhizobiales, B. abortus displays unipolar growth from the new cell pole generated by cell division. We found that the phosphodiesterase PdeA, the ortholog of the essential polar growth factor RgsP of the Rhizobiale Sinorhizobium meliloti, is required for rod shape integrity but is not essential for B. abortus growth. Indeed, the radius of the pole is increased by 31 ± 1.7% in a ΔpdeA mutant, generating a coccoid morphology. A mutation in the cyclic-di-GMP phosphodiesterase catalytic site of PdeA does not generate the coccoid morphology and the ΔpdeA mutant kept the ability to recruit markers of new and old poles. However, the presence of PdeA is required in an intra-nasal mouse model of infection. In conclusion, we propose that PdeA contributes to bacterial morphology and virulence in B. abortus, but it is not crucial for polarity and asymmetric growth.

Original languageEnglish
Pages (from-to)1449-1463
Number of pages15
JournalMolecular Microbiology
Volume116
Issue number6
Early online date18 Oct 2021
DOIs
Publication statusPublished - Dec 2021

Keywords

  • Alpha-Proteobacteria
  • Brucella
  • Rhizobiales
  • asymmetric growth
  • cell shape
  • cyclic-di-GMP

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