Pancreatic islet structure and function in JCR:LA-corpulent rats

Karim Louchami, Charles Nicaise, Nurdan Bulur, Emeline Hupkens, Willy J Malaisse, Abdullah Sener

Research output: Contribution to journalArticle

3 Downloads (Pure)

Abstract

JCR-LA-corpulent rats, homozygote for the cp gene, represent a current animal model for obesity and hyperlipemia. The
present study refers mainly to secretory and histological findings made in the islets of these rats. Male fed Wistar rats and both
lean and obese LA/N rats were killed under CO2 anesthesia. Histological data were obtained in pancreatic sections. Pancreatic
islets were isolated by the collagenase procedure. Despite a higher age, the lean LA/N rats displayed lower body weight and
epididymal fat weight than control Wistar rats. Their plasma glucose and insulin concentrations, plasma insulin/glucose ratio,
HOMA for insulin resistance, islet mean size (μm²) and basal insulin output by islets exposed to 2.8 mM glucose did not differ
significantly from those recorded in the control Wistar rats. Over 90 min incubation at 16.7 mM glucose, however, the release of
insulin was 28.7 % higher (p < 0.005) from the islets of lean LA/N rats as compared to Wistar rats, despite a lower islet protein
and insulin content (p < 0.05) in the former than latter animals. When compared to lean LA/N rats of similar age, the obese
LA/N rats displayed higher body weight, 5-fold higher epididymal fat weight, and abnormally high plasma glucose and insulin
concentrations, plasma insulin/glucose ratio and insulin times glucose product. The number of islets per mm² was twice higher,
their mean size 5 times higher, and the basal insulin output twice higher in obese, as compared to lean, LA/N rats. At 16.7 mM
glucose, the insulin output/content ratio was also twice higher in obese compared to lean LA/N rats. The immunohistochemical
staining of the islets for GLUT2 yielded a patchy distribution in the islets of obese LA/N rats as distinct from a homogenously
dense pattern in the islets of either lean LA/N rats or Wistar rats. The present results indicate that the total islet volume is about
22 times higher in obese than in lean LA/N rats, the former rats being eventually considered as suitable for non-invasive
imaging and quantification of pancreatic insulin-producing cells in an animal model with severely increased ß-cell mass.
Original languageEnglish
Pages (from-to)9-14
Number of pages6
JournalMetabolic and Functional Research on Diabetes
Publication statusPublished - 2010

Fingerprint

Islets of Langerhans
Insulin
Glucose
Wistar Rats
Animal Models
Fats
Body Weight
Weights and Measures
Homozygote
Collagenases
Hyperlipidemias
Insulin Resistance
Anesthesia
Obesity

Keywords

  • JCR:LA-cp rats
  • insulin resistance
  • hyperinsulinism
  • isolated pancreatic islets
  • insulin secretion
  • pancreatic ßcell mass

Cite this

Louchami, K., Nicaise, C., Bulur, N., Hupkens, E., Malaisse, W. J., & Sener, A. (2010). Pancreatic islet structure and function in JCR:LA-corpulent rats. Metabolic and Functional Research on Diabetes, 9-14.
Louchami, Karim ; Nicaise, Charles ; Bulur, Nurdan ; Hupkens, Emeline ; Malaisse, Willy J ; Sener, Abdullah. / Pancreatic islet structure and function in JCR:LA-corpulent rats. In: Metabolic and Functional Research on Diabetes. 2010 ; pp. 9-14.
@article{f8d824edecd14bdd90f85fbe16befcba,
title = "Pancreatic islet structure and function in JCR:LA-corpulent rats",
abstract = "JCR-LA-corpulent rats, homozygote for the cp gene, represent a current animal model for obesity and hyperlipemia. Thepresent study refers mainly to secretory and histological findings made in the islets of these rats. Male fed Wistar rats and bothlean and obese LA/N rats were killed under CO2 anesthesia. Histological data were obtained in pancreatic sections. Pancreaticislets were isolated by the collagenase procedure. Despite a higher age, the lean LA/N rats displayed lower body weight andepididymal fat weight than control Wistar rats. Their plasma glucose and insulin concentrations, plasma insulin/glucose ratio,HOMA for insulin resistance, islet mean size (μm²) and basal insulin output by islets exposed to 2.8 mM glucose did not differsignificantly from those recorded in the control Wistar rats. Over 90 min incubation at 16.7 mM glucose, however, the release ofinsulin was 28.7 {\%} higher (p < 0.005) from the islets of lean LA/N rats as compared to Wistar rats, despite a lower islet proteinand insulin content (p < 0.05) in the former than latter animals. When compared to lean LA/N rats of similar age, the obeseLA/N rats displayed higher body weight, 5-fold higher epididymal fat weight, and abnormally high plasma glucose and insulinconcentrations, plasma insulin/glucose ratio and insulin times glucose product. The number of islets per mm² was twice higher,their mean size 5 times higher, and the basal insulin output twice higher in obese, as compared to lean, LA/N rats. At 16.7 mMglucose, the insulin output/content ratio was also twice higher in obese compared to lean LA/N rats. The immunohistochemicalstaining of the islets for GLUT2 yielded a patchy distribution in the islets of obese LA/N rats as distinct from a homogenouslydense pattern in the islets of either lean LA/N rats or Wistar rats. The present results indicate that the total islet volume is about22 times higher in obese than in lean LA/N rats, the former rats being eventually considered as suitable for non-invasiveimaging and quantification of pancreatic insulin-producing cells in an animal model with severely increased {\ss}-cell mass.",
keywords = "JCR:LA-cp rats, insulin resistance, hyperinsulinism, isolated pancreatic islets, insulin secretion, pancreatic {\ss}cell mass",
author = "Karim Louchami and Charles Nicaise and Nurdan Bulur and Emeline Hupkens and Malaisse, {Willy J} and Abdullah Sener",
year = "2010",
language = "English",
pages = "9--14",
journal = "Metabolic and Functional Research on Diabetes",
issn = "2030-1596",

}

Pancreatic islet structure and function in JCR:LA-corpulent rats. / Louchami, Karim; Nicaise, Charles; Bulur, Nurdan; Hupkens, Emeline; Malaisse, Willy J; Sener, Abdullah.

In: Metabolic and Functional Research on Diabetes, 2010, p. 9-14.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Pancreatic islet structure and function in JCR:LA-corpulent rats

AU - Louchami, Karim

AU - Nicaise, Charles

AU - Bulur, Nurdan

AU - Hupkens, Emeline

AU - Malaisse, Willy J

AU - Sener, Abdullah

PY - 2010

Y1 - 2010

N2 - JCR-LA-corpulent rats, homozygote for the cp gene, represent a current animal model for obesity and hyperlipemia. Thepresent study refers mainly to secretory and histological findings made in the islets of these rats. Male fed Wistar rats and bothlean and obese LA/N rats were killed under CO2 anesthesia. Histological data were obtained in pancreatic sections. Pancreaticislets were isolated by the collagenase procedure. Despite a higher age, the lean LA/N rats displayed lower body weight andepididymal fat weight than control Wistar rats. Their plasma glucose and insulin concentrations, plasma insulin/glucose ratio,HOMA for insulin resistance, islet mean size (μm²) and basal insulin output by islets exposed to 2.8 mM glucose did not differsignificantly from those recorded in the control Wistar rats. Over 90 min incubation at 16.7 mM glucose, however, the release ofinsulin was 28.7 % higher (p < 0.005) from the islets of lean LA/N rats as compared to Wistar rats, despite a lower islet proteinand insulin content (p < 0.05) in the former than latter animals. When compared to lean LA/N rats of similar age, the obeseLA/N rats displayed higher body weight, 5-fold higher epididymal fat weight, and abnormally high plasma glucose and insulinconcentrations, plasma insulin/glucose ratio and insulin times glucose product. The number of islets per mm² was twice higher,their mean size 5 times higher, and the basal insulin output twice higher in obese, as compared to lean, LA/N rats. At 16.7 mMglucose, the insulin output/content ratio was also twice higher in obese compared to lean LA/N rats. The immunohistochemicalstaining of the islets for GLUT2 yielded a patchy distribution in the islets of obese LA/N rats as distinct from a homogenouslydense pattern in the islets of either lean LA/N rats or Wistar rats. The present results indicate that the total islet volume is about22 times higher in obese than in lean LA/N rats, the former rats being eventually considered as suitable for non-invasiveimaging and quantification of pancreatic insulin-producing cells in an animal model with severely increased ß-cell mass.

AB - JCR-LA-corpulent rats, homozygote for the cp gene, represent a current animal model for obesity and hyperlipemia. Thepresent study refers mainly to secretory and histological findings made in the islets of these rats. Male fed Wistar rats and bothlean and obese LA/N rats were killed under CO2 anesthesia. Histological data were obtained in pancreatic sections. Pancreaticislets were isolated by the collagenase procedure. Despite a higher age, the lean LA/N rats displayed lower body weight andepididymal fat weight than control Wistar rats. Their plasma glucose and insulin concentrations, plasma insulin/glucose ratio,HOMA for insulin resistance, islet mean size (μm²) and basal insulin output by islets exposed to 2.8 mM glucose did not differsignificantly from those recorded in the control Wistar rats. Over 90 min incubation at 16.7 mM glucose, however, the release ofinsulin was 28.7 % higher (p < 0.005) from the islets of lean LA/N rats as compared to Wistar rats, despite a lower islet proteinand insulin content (p < 0.05) in the former than latter animals. When compared to lean LA/N rats of similar age, the obeseLA/N rats displayed higher body weight, 5-fold higher epididymal fat weight, and abnormally high plasma glucose and insulinconcentrations, plasma insulin/glucose ratio and insulin times glucose product. The number of islets per mm² was twice higher,their mean size 5 times higher, and the basal insulin output twice higher in obese, as compared to lean, LA/N rats. At 16.7 mMglucose, the insulin output/content ratio was also twice higher in obese compared to lean LA/N rats. The immunohistochemicalstaining of the islets for GLUT2 yielded a patchy distribution in the islets of obese LA/N rats as distinct from a homogenouslydense pattern in the islets of either lean LA/N rats or Wistar rats. The present results indicate that the total islet volume is about22 times higher in obese than in lean LA/N rats, the former rats being eventually considered as suitable for non-invasiveimaging and quantification of pancreatic insulin-producing cells in an animal model with severely increased ß-cell mass.

KW - JCR:LA-cp rats

KW - insulin resistance

KW - hyperinsulinism

KW - isolated pancreatic islets

KW - insulin secretion

KW - pancreatic ßcell mass

M3 - Article

SP - 9

EP - 14

JO - Metabolic and Functional Research on Diabetes

JF - Metabolic and Functional Research on Diabetes

SN - 2030-1596

ER -