TY - JOUR
T1 - Overexpression, purification, crystallization and crystallographic analysis of CopK of Cupriavidus metallidurans
AU - Tricot, Catherine
AU - Van Aelst, Sebastien
AU - Wattiez, Ruddy
AU - Mergeay, Max
AU - Stalon, Victor
AU - Wouters, Johan
PY - 2005/9/1
Y1 - 2005/9/1
N2 - CopK of Cupriavidus metallidurans is a 93-amino-acid protein whose mature form (73 amino acids) has been purified and crystallized by the hanging-drop vapour-diffusion method in 100 mM citrate pH 3.5, 200 mM Li2SO 4, 20%(w/v) glycerol, 13%(w/v) PEG 8000. Crystals display orthorhombic symmetry, with unit-cell parameters a = 57.53, b = 128.65, c = 49.77 Å, and diffract to 2.2 Å resolution using synchrotron radiation.
AB - CopK of Cupriavidus metallidurans is a 93-amino-acid protein whose mature form (73 amino acids) has been purified and crystallized by the hanging-drop vapour-diffusion method in 100 mM citrate pH 3.5, 200 mM Li2SO 4, 20%(w/v) glycerol, 13%(w/v) PEG 8000. Crystals display orthorhombic symmetry, with unit-cell parameters a = 57.53, b = 128.65, c = 49.77 Å, and diffract to 2.2 Å resolution using synchrotron radiation.
UR - http://www.scopus.com/inward/record.url?scp=33646351485&partnerID=8YFLogxK
U2 - 10.1107/S174430910502316X
DO - 10.1107/S174430910502316X
M3 - Article
C2 - 16511169
AN - SCOPUS:33646351485
SN - 1744-3091
VL - 61
SP - 825
EP - 827
JO - Acta Crystallographica Section F
JF - Acta Crystallographica Section F
IS - 9
ER -