Overexpression, purification, crystallization and crystallographic analysis of CopK of Cupriavidus metallidurans

Catherine Tricot, Sebastien Van Aelst, Ruddy Wattiez, Max Mergeay, Victor Stalon, Johan Wouters

Research output: Contribution to journalArticle

Abstract

CopK of Cupriavidus metallidurans is a 93-amino-acid protein whose mature form (73 amino acids) has been purified and crystallized by the hanging-drop vapour-diffusion method in 100 mM citrate pH 3.5, 200 mM Li2SO 4, 20%(w/v) glycerol, 13%(w/v) PEG 8000. Crystals display orthorhombic symmetry, with unit-cell parameters a = 57.53, b = 128.65, c = 49.77 Å, and diffract to 2.2 Å resolution using synchrotron radiation.

Original languageEnglish
Pages (from-to)825-827
Number of pages3
JournalActa Crystallographica Section F
Volume61
Issue number9
DOIs
Publication statusPublished - 1 Sep 2005

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