Abstract
The orientation of antibodies
upon their immobilisation on different surfacesis a
crucial factor affecting their recognition properties.
This phenomenon is strongly dependent on surface
properties such as composition, reactivity,
wettability, roughness etc. [1-2]. Some polymeric
substrates, such as Zeonor, are used in bio-medical
or -technological devices, mainly because of their
interesting optical properties, low production time
and cost. However, their surface
modification/functionalisation is more difficult to
perform as compared to other widely studied
metallic substrates (such as gold or silicon).
Our objective is to overcome these problems by
performing a two-step experiment. The first step
uses the advantages of well-known modified
metallic/semiconducting substrates (in particular
organosilane-modified silicon) for the orientation
of antibodies [3-4]. The second step consists of
transferring the oriented antibodies onto a second
substrate, which may have unfavourable
characteristics. This transfer process is based on
the template-imprinted nanostructures technique
developed by Ratner et al. [5]. In this poster, we
focus in particular on the transfer process of
proteins from one substrate to the other.
| Original language | English |
|---|---|
| Pages (from-to) | 42-42 |
| Number of pages | 1 |
| Journal | European Cells and Materials |
| Volume | 14 |
| Issue number | Suppl. 3 |
| Publication status | Published - 2007 |