Abstract
It is assumed that intracellular pathogenic bacteria have to cope with DNA alkylating stress within host cells. Here we use single-cell reporter systems to show that the pathogen Brucella abortus does encounter alkylating stress during the first hours of macrophage infection. Genes encoding direct repair and base-excision repair pathways are required by B. abortus to face this stress in vitro and in a mouse infection model. Among these genes, ogt is found to be under the control of the conserved cell-cycle transcription factor GcrA. Our results highlight that the control of DNA repair in B. abortus displays distinct features that are not present in model organisms such as Escherichia coli.
Original language | English |
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Article number | 4847 |
Pages (from-to) | 4847 |
Journal | Nature Communications |
Volume | 10 |
Issue number | 1 |
DOIs | |
Publication status | Published - 24 Oct 2019 |
Funding
We thank members of the URBM and I. Matic for stimulating discussions. We also thank Y. Ashhab for his help regarding ChIP-seq analysis. We thank H. Lavender and C. M. Tang for their careful reading of our manuscript. We thank UNamur (https://www.unamur.be/) for financial and logistic support. This work was funded by The Fédération Wallonie-Bruxelles (ARC 17/22-087) and by the FRS-FNRS “Brucell-cycle” (PDR T.0060.15). K.P., A.M., and G.P. were supported by a FRIA Ph.D. fellowship. A.R. and N.F. held an Aspirant fellowship from FRS-FNRS. This work was also supported by the French Agence Nationale de Recherche (ANR-JCJC-2011-Castacc) (http://www.agence-nationale-recherche.fr/) and the Region Pas-De-Calais (http://www.nordpasdecalais.fr) CPER to A.F. and E.G.B.
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Biological Security Laboratory Level 3 (BL3)
De Bolle, X. (Manager)
Technological Platform: Biological Security Laboratory Level 3Facility/equipment: Technological Platform