Occurrence and repair of alkylating stress in the intracellular pathogen Brucella abortus

Research output: Contribution to journalArticle

Abstract

It is assumed that intracellular pathogenic bacteria have to cope with DNA alkylating stress within host cells. Here we use single-cell reporter systems to show that the pathogen Brucella abortus does encounter alkylating stress during the first hours of macrophage infection. Genes encoding direct repair and base-excision repair pathways are required by B. abortus to face this stress in vitro and in a mouse infection model. Among these genes, ogt is found to be under the control of the conserved cell-cycle transcription factor GcrA. Our results highlight that the control of DNA repair in B. abortus displays distinct features that are not present in model organisms such as Escherichia coli.

Original languageEnglish
Article number4847
Pages (from-to)4847
JournalNature Communications
Volume10
Issue number1
DOIs
Publication statusPublished - 24 Oct 2019

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Brucella abortus
pathogens
Pathogens
Repair
occurrences
infectious diseases
DNA Repair
genes
deoxyribonucleic acid
Gene encoding
macrophages
Macrophages
DNA
Escherichia
Cell Cycle Checkpoints
Infection
cells
organisms
encounters
Escherichia coli

Cite this

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title = "Occurrence and repair of alkylating stress in the intracellular pathogen Brucella abortus",
abstract = "It is assumed that intracellular pathogenic bacteria have to cope with DNA alkylating stress within host cells. Here we use single-cell reporter systems to show that the pathogen Brucella abortus does encounter alkylating stress during the first hours of macrophage infection. Genes encoding direct repair and base-excision repair pathways are required by B. abortus to face this stress in vitro and in a mouse infection model. Among these genes, ogt is found to be under the control of the conserved cell-cycle transcription factor GcrA. Our results highlight that the control of DNA repair in B. abortus displays distinct features that are not present in model organisms such as Escherichia coli.",
author = "Katy Poncin and Agn{\`e}s Roba and Ravikumar Jimmidi and Georges Potemberg and Antonella Fioravanti and Nayla Francis and K{\'e}vin Willemart and Nicolas Zeippen and Arnaud Machelart and Biondi, {Emanuele G.} and Eric Muraille and Vincent, {St{\'e}phane P.} and {De Bolle}, Xavier",
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T1 - Occurrence and repair of alkylating stress in the intracellular pathogen Brucella abortus

AU - Poncin, Katy

AU - Roba, Agnès

AU - Jimmidi, Ravikumar

AU - Potemberg, Georges

AU - Fioravanti, Antonella

AU - Francis, Nayla

AU - Willemart, Kévin

AU - Zeippen, Nicolas

AU - Machelart, Arnaud

AU - Biondi, Emanuele G.

AU - Muraille, Eric

AU - Vincent, Stéphane P.

AU - De Bolle, Xavier

PY - 2019/10/24

Y1 - 2019/10/24

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