Abstract
It is assumed that intracellular pathogenic bacteria have to cope with DNA alkylating stress within host cells. Here we use single-cell reporter systems to show that the pathogen Brucella abortus does encounter alkylating stress during the first hours of macrophage infection. Genes encoding direct repair and base-excision repair pathways are required by B. abortus to face this stress in vitro and in a mouse infection model. Among these genes, ogt is found to be under the control of the conserved cell-cycle transcription factor GcrA. Our results highlight that the control of DNA repair in B. abortus displays distinct features that are not present in model organisms such as Escherichia coli.
| Original language | English |
|---|---|
| Article number | 4847 |
| Pages (from-to) | 4847 |
| Journal | Nature Communications |
| Volume | 10 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 24 Oct 2019 |
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Occurrence and repair of alkylating stress in the intracellular pathogen Brucella abortus. / Poncin, Katy; Roba, Agnès; Jimmidi, Ravikumar; Potemberg, Georges; Fioravanti, Antonella; Francis, Nayla; Willemart, Kévin; Zeippen, Nicolas; Machelart, Arnaud; Biondi, Emanuele G.; Muraille, Eric; Vincent, Stéphane P.; De Bolle, Xavier.
In: Nature Communications, Vol. 10, No. 1, 4847, 24.10.2019, p. 4847.Research output: Contribution to journal › Article
TY - JOUR
T1 - Occurrence and repair of alkylating stress in the intracellular pathogen Brucella abortus
AU - Poncin, Katy
AU - Roba, Agnès
AU - Jimmidi, Ravikumar
AU - Potemberg, Georges
AU - Fioravanti, Antonella
AU - Francis, Nayla
AU - Willemart, Kévin
AU - Zeippen, Nicolas
AU - Machelart, Arnaud
AU - Biondi, Emanuele G.
AU - Muraille, Eric
AU - Vincent, Stéphane P.
AU - De Bolle, Xavier
PY - 2019/10/24
Y1 - 2019/10/24
N2 - It is assumed that intracellular pathogenic bacteria have to cope with DNA alkylating stress within host cells. Here we use single-cell reporter systems to show that the pathogen Brucella abortus does encounter alkylating stress during the first hours of macrophage infection. Genes encoding direct repair and base-excision repair pathways are required by B. abortus to face this stress in vitro and in a mouse infection model. Among these genes, ogt is found to be under the control of the conserved cell-cycle transcription factor GcrA. Our results highlight that the control of DNA repair in B. abortus displays distinct features that are not present in model organisms such as Escherichia coli.
AB - It is assumed that intracellular pathogenic bacteria have to cope with DNA alkylating stress within host cells. Here we use single-cell reporter systems to show that the pathogen Brucella abortus does encounter alkylating stress during the first hours of macrophage infection. Genes encoding direct repair and base-excision repair pathways are required by B. abortus to face this stress in vitro and in a mouse infection model. Among these genes, ogt is found to be under the control of the conserved cell-cycle transcription factor GcrA. Our results highlight that the control of DNA repair in B. abortus displays distinct features that are not present in model organisms such as Escherichia coli.
UR - http://www.scopus.com/inward/record.url?scp=85074091344&partnerID=8YFLogxK
U2 - 10.1038/s41467-019-12516-8
DO - 10.1038/s41467-019-12516-8
M3 - Article
C2 - 31649248
AN - SCOPUS:85074091344
VL - 10
SP - 4847
JO - Nature Communications
JF - Nature Communications
SN - 2041-1723
IS - 1
M1 - 4847
ER -