Nonlinear optical spectroscopy and two-photon excited fluorescence spectroscopy reveal the excited states of fluorophores embedded in a beetle's elytra

Sébastien R Mouchet, Charlotte Verstraete, Dimitrije Mara, Stijn Van Cleuvenbergen, Ewan D Finlayson, Rik Van Deun, Olivier Deparis, Thierry Verbiest, Bjorn Maes, Pete Vukusic, Branko Kolaric

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Abstract

Upon illumination by ultraviolet light, many animal species emit light through fluorescence processes arising from fluorophores embedded within their biological tissues. Fluorescence studies in living organisms are however relatively scarce and so far limited to the linear regime. Multiphoton excitation fluorescence analyses as well as nonlinear optical techniques offer unique possibilities to investigate the effects of the local environment on the excited states of fluorophores. Herein, these techniques are applied for the first time to study of the naturally controlled fluorescence in insects. The case of the male Hoplia coerulea beetle is investigated because the scales covering the beetle's elytra are known to possess an internal photonic structure with embedded fluorophores, which controls both the beetle's coloration and the fluorescence emission. An intense two-photon excitation fluorescence signal is observed, the intensity of which changes upon contact with water. A third-harmonic generation signal is also detected, the intensity of which depends on the light polarization state. The analysis of these nonlinear optical and fluorescent responses unveils the multi-excited states character of the fluorophore molecules embedded in the beetle's elytra. The role of form anisotropy in the photonic structure, which causes additional tailoring of the beetle's optical responses, is demonstrated by circularly polarized light and nonlinear optical measurements.

Original languageEnglish
Article number20180052
Pages (from-to)20180052
Number of pages8
JournalInterface focus
Volume9
Issue number1
DOIs
Publication statusPublished - 14 Dec 2018

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Fluorescence Spectrometry
Beetles
Photons
Spectrum Analysis
Fluorescence
Optics and Photonics
Light
Anisotropy
Ultraviolet Rays
Lighting
Insects
Water

Keywords

  • natural photonics
  • photonics crystals
  • fluorescence
  • two-photon fluorescence
  • third-harmonic generation
  • Beetle

Cite this

Mouchet, Sébastien R ; Verstraete, Charlotte ; Mara, Dimitrije ; Van Cleuvenbergen, Stijn ; Finlayson, Ewan D ; Van Deun, Rik ; Deparis, Olivier ; Verbiest, Thierry ; Maes, Bjorn ; Vukusic, Pete ; Kolaric, Branko. / Nonlinear optical spectroscopy and two-photon excited fluorescence spectroscopy reveal the excited states of fluorophores embedded in a beetle's elytra. In: Interface focus. 2018 ; Vol. 9, No. 1. pp. 20180052.
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abstract = "Upon illumination by ultraviolet light, many animal species emit light through fluorescence processes arising from fluorophores embedded within their biological tissues. Fluorescence studies in living organisms are however relatively scarce and so far limited to the linear regime. Multiphoton excitation fluorescence analyses as well as nonlinear optical techniques offer unique possibilities to investigate the effects of the local environment on the excited states of fluorophores. Herein, these techniques are applied for the first time to study of the naturally controlled fluorescence in insects. The case of the male Hoplia coerulea beetle is investigated because the scales covering the beetle's elytra are known to possess an internal photonic structure with embedded fluorophores, which controls both the beetle's coloration and the fluorescence emission. An intense two-photon excitation fluorescence signal is observed, the intensity of which changes upon contact with water. A third-harmonic generation signal is also detected, the intensity of which depends on the light polarization state. The analysis of these nonlinear optical and fluorescent responses unveils the multi-excited states character of the fluorophore molecules embedded in the beetle's elytra. The role of form anisotropy in the photonic structure, which causes additional tailoring of the beetle's optical responses, is demonstrated by circularly polarized light and nonlinear optical measurements.",
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Mouchet, SR, Verstraete, C, Mara, D, Van Cleuvenbergen, S, Finlayson, ED, Van Deun, R, Deparis, O, Verbiest, T, Maes, B, Vukusic, P & Kolaric, B 2018, 'Nonlinear optical spectroscopy and two-photon excited fluorescence spectroscopy reveal the excited states of fluorophores embedded in a beetle's elytra', Interface focus, vol. 9, no. 1, 20180052, pp. 20180052. https://doi.org/10.1098/rsfs.2018.0052

Nonlinear optical spectroscopy and two-photon excited fluorescence spectroscopy reveal the excited states of fluorophores embedded in a beetle's elytra. / Mouchet, Sébastien R; Verstraete, Charlotte; Mara, Dimitrije; Van Cleuvenbergen, Stijn; Finlayson, Ewan D; Van Deun, Rik; Deparis, Olivier; Verbiest, Thierry; Maes, Bjorn; Vukusic, Pete; Kolaric, Branko.

In: Interface focus, Vol. 9, No. 1, 20180052, 14.12.2018, p. 20180052.

Research output: Contribution to journalArticle

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T1 - Nonlinear optical spectroscopy and two-photon excited fluorescence spectroscopy reveal the excited states of fluorophores embedded in a beetle's elytra

AU - Mouchet, Sébastien R

AU - Verstraete, Charlotte

AU - Mara, Dimitrije

AU - Van Cleuvenbergen, Stijn

AU - Finlayson, Ewan D

AU - Van Deun, Rik

AU - Deparis, Olivier

AU - Verbiest, Thierry

AU - Maes, Bjorn

AU - Vukusic, Pete

AU - Kolaric, Branko

PY - 2018/12/14

Y1 - 2018/12/14

N2 - Upon illumination by ultraviolet light, many animal species emit light through fluorescence processes arising from fluorophores embedded within their biological tissues. Fluorescence studies in living organisms are however relatively scarce and so far limited to the linear regime. Multiphoton excitation fluorescence analyses as well as nonlinear optical techniques offer unique possibilities to investigate the effects of the local environment on the excited states of fluorophores. Herein, these techniques are applied for the first time to study of the naturally controlled fluorescence in insects. The case of the male Hoplia coerulea beetle is investigated because the scales covering the beetle's elytra are known to possess an internal photonic structure with embedded fluorophores, which controls both the beetle's coloration and the fluorescence emission. An intense two-photon excitation fluorescence signal is observed, the intensity of which changes upon contact with water. A third-harmonic generation signal is also detected, the intensity of which depends on the light polarization state. The analysis of these nonlinear optical and fluorescent responses unveils the multi-excited states character of the fluorophore molecules embedded in the beetle's elytra. The role of form anisotropy in the photonic structure, which causes additional tailoring of the beetle's optical responses, is demonstrated by circularly polarized light and nonlinear optical measurements.

AB - Upon illumination by ultraviolet light, many animal species emit light through fluorescence processes arising from fluorophores embedded within their biological tissues. Fluorescence studies in living organisms are however relatively scarce and so far limited to the linear regime. Multiphoton excitation fluorescence analyses as well as nonlinear optical techniques offer unique possibilities to investigate the effects of the local environment on the excited states of fluorophores. Herein, these techniques are applied for the first time to study of the naturally controlled fluorescence in insects. The case of the male Hoplia coerulea beetle is investigated because the scales covering the beetle's elytra are known to possess an internal photonic structure with embedded fluorophores, which controls both the beetle's coloration and the fluorescence emission. An intense two-photon excitation fluorescence signal is observed, the intensity of which changes upon contact with water. A third-harmonic generation signal is also detected, the intensity of which depends on the light polarization state. The analysis of these nonlinear optical and fluorescent responses unveils the multi-excited states character of the fluorophore molecules embedded in the beetle's elytra. The role of form anisotropy in the photonic structure, which causes additional tailoring of the beetle's optical responses, is demonstrated by circularly polarized light and nonlinear optical measurements.

KW - natural photonics

KW - photonics crystals

KW - fluorescence

KW - two-photon fluorescence

KW - third-harmonic generation

KW - Beetle

U2 - 10.1098/rsfs.2018.0052

DO - 10.1098/rsfs.2018.0052

M3 - Article

VL - 9

SP - 20180052

JO - Interface focus

JF - Interface focus

SN - 2042-8898

IS - 1

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