Nonlinear optical properties of mStrawberry and mCherry for second harmonic imaging

E. De Meulenaere, M. de Wergifosse, E. Botek, S. Spaepen, B. Champagne, J. Vanderleyden, K. Clays

Research output: Contribution to journalArticle

Abstract

The second-order nonlinear optical properties of two monomeric red fluorescent proteins, mStrawberry and mCherry, have been experimentally determined by frequency-resolved femtosecond hyper-Rayleigh scattering. These proteins were found to exhibit a stronger nonlinear response than the previously described eGFP, eYFP and DsRed, confirming the trend that fluorophores with a more extended conjugated system, or a lower-energy band gap between ground and excited state, exhibit a larger static hyperpolarizability (β). Furthermore, these experimental data were complemented with quantum chemical calculations. A discrepancy was observed between experimental and theoretical results, but this could be explained by the chromophore model extracted from the available X-ray diffraction data. While eGFP showed a larger dynamic experimental response (β) due to the highest resonance enhancement, we measured an even higher signal for mCherry. Furthermore, mCherry also shows a better separation of the second harmonic signal and two-photon excited fluorescent signal, making this the preferred fluorescent protein for second harmonic imaging at 800 nm so far.
Original languageEnglish
Pages (from-to)1-13
Number of pages13
JournalJournal of Nonlinear Optical Physics and Materials
Volume19
Issue number1
DOIs
Publication statusPublished - 2010

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Optical properties
proteins
Proteins
harmonics
Imaging techniques
optical properties
Rayleigh scattering
Fluorophores
Chromophores
Excited states
Band structure
Ground state
Energy gap
Photons
X ray diffraction
chromophores
energy bands
trends
ground state
augmentation

Cite this

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title = "Nonlinear optical properties of mStrawberry and mCherry for second harmonic imaging",
abstract = "The second-order nonlinear optical properties of two monomeric red fluorescent proteins, mStrawberry and mCherry, have been experimentally determined by frequency-resolved femtosecond hyper-Rayleigh scattering. These proteins were found to exhibit a stronger nonlinear response than the previously described eGFP, eYFP and DsRed, confirming the trend that fluorophores with a more extended conjugated system, or a lower-energy band gap between ground and excited state, exhibit a larger static hyperpolarizability (β). Furthermore, these experimental data were complemented with quantum chemical calculations. A discrepancy was observed between experimental and theoretical results, but this could be explained by the chromophore model extracted from the available X-ray diffraction data. While eGFP showed a larger dynamic experimental response (β) due to the highest resonance enhancement, we measured an even higher signal for mCherry. Furthermore, mCherry also shows a better separation of the second harmonic signal and two-photon excited fluorescent signal, making this the preferred fluorescent protein for second harmonic imaging at 800 nm so far.",
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Nonlinear optical properties of mStrawberry and mCherry for second harmonic imaging. / De Meulenaere, E.; de Wergifosse, M.; Botek, E.; Spaepen, S.; Champagne, B.; Vanderleyden, J.; Clays, K.

In: Journal of Nonlinear Optical Physics and Materials, Vol. 19, No. 1, 2010, p. 1-13.

Research output: Contribution to journalArticle

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AU - De Meulenaere, E.

AU - de Wergifosse, M.

AU - Botek, E.

AU - Spaepen, S.

AU - Champagne, B.

AU - Vanderleyden, J.

AU - Clays, K.

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