Activities per year
Abstract
Membrane lipid raft model has long been debated, but recently the concept of lipid submicrometric domains has emerged to characterize larger (micrometric) and more stable lipid membrane domains. Such domains organize signaling platforms involved in normal or pathological conditions. In this study, adhering human keratinocytes were investigated for their ability to organize such specialized lipid domains. Successful fluorescent probing of lipid domains, by either inserting exogenous sphingomyelin (BODIPY-SM) or using detoxified fragments of lysenin and theta toxins fused to mCherry, allowed specific, sensitive and quantitative detection of sphingomyelin and cholesterol and demonstrated for the first time submicrometric organization of lipid domains in living keratinocytes. Potential functionality of such domains was additionally assessed during replicative senescence, notably through gradual disappearance of SM-rich domains in senescent keratinocytes. Indeed, SM-rich domains were found critical to preserve keratinocyte migration before senescence, because sphingomyelin or cholesterol depletion in keratinocytes significantly alters lipid domains and reduce migration ability.
Original language | English |
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Pages (from-to) | 958-971 |
Number of pages | 14 |
Journal | Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids |
Volume | 1862 |
Issue number | 9 |
DOIs | |
Publication status | Published - 23 Jun 2017 |
Keywords
- Journal Article
- Senescence
- Lipid submicrometric domains
- Keratinocytes
- Sphingomyelin
- Keratinocyte migration
- Cholesterol
- Sphingomyelins/metabolism
- Lipids/physiology
- Membrane Microdomains/metabolism
- Cholesterol/metabolism
- Humans
- Cells, Cultured
- Keratinocytes/metabolism
- Cell Movement/physiology
- Cell Membrane/metabolism
- Re-Epithelialization/physiology
- Membrane Lipids/metabolism
- Toxins, Biological/metabolism
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Morphology - Imaging
Cecchet, F. (Manager) & Renard, H.-F. (Manager)
Technological Platform Morphology - ImagingFacility/equipment: Technological Platform
Activities
- 1 External Thesis
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How cytoskeletal impairment in hereditary elliptocytosis and spherocytosis affe"cts erythrocyte membrane composiiton, organization and deformation
Poumay, Y. (Jury Member)
3 Jul 2019Activity: Examinations › External Thesis