Non-senescent keratinocytes organize in plasma membrane submicrometric lipid domains enriched in sphingomyelin and involved in re-epithelialization

Abdallah Mound, Vesela Lozanova, Céline Warnon, Maryse Hermant, Julie Robic, Christelle Guere, Katell Vie, Catherine Lambert de Rouvroit, Donatienne Tyteca, Florence Debacq-Chainiaux, Yves Poumay

Research output: Contribution to journalArticle

Abstract

Membrane lipid raft model has long been debated, but recently the concept of lipid submicrometric domains has emerged to characterize larger (micrometric) and more stable lipid membrane domains. Such domains organize signaling platforms involved in normal or pathological conditions. In this study, adhering human keratinocytes were investigated for their ability to organize such specialized lipid domains. Successful fluorescent probing of lipid domains, by either inserting exogenous sphingomyelin (BODIPY-SM) or using detoxified fragments of lysenin and theta toxins fused to mCherry, allowed specific, sensitive and quantitative detection of sphingomyelin and cholesterol and demonstrated for the first time submicrometric organization of lipid domains in living keratinocytes. Potential functionality of such domains was additionally assessed during replicative senescence, notably through gradual disappearance of SM-rich domains in senescent keratinocytes. Indeed, SM-rich domains were found critical to preserve keratinocyte migration before senescence, because sphingomyelin or cholesterol depletion in keratinocytes significantly alters lipid domains and reduce migration ability.

Original languageEnglish
Pages (from-to)958-971
Number of pages14
JournalBiochimica Biophysica Acta.
Volume1862
Issue number9
DOIs
Publication statusPublished - 23 Jun 2017

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Re-Epithelialization
Sphingomyelins
Membrane Lipids
Keratinocytes
Cell Membrane
Lipids
Cholesterol
Cell Aging

Keywords

  • Journal Article
  • Senescence
  • Lipid submicrometric domains
  • Keratinocytes
  • Sphingomyelin
  • Keratinocyte migration
  • Cholesterol
  • Sphingomyelins/metabolism
  • Lipids/physiology
  • Membrane Microdomains/metabolism
  • Cholesterol/metabolism
  • Humans
  • Cells, Cultured
  • Keratinocytes/metabolism
  • Cell Movement/physiology
  • Cell Membrane/metabolism
  • Re-Epithelialization/physiology
  • Membrane Lipids/metabolism
  • Toxins, Biological/metabolism

Cite this

@article{07d908b03c1343778355db6ea536bb2c,
title = "Non-senescent keratinocytes organize in plasma membrane submicrometric lipid domains enriched in sphingomyelin and involved in re-epithelialization",
abstract = "Membrane lipid raft model has long been debated, but recently the concept of lipid submicrometric domains has emerged to characterize larger (micrometric) and more stable lipid membrane domains. Such domains organize signaling platforms involved in normal or pathological conditions. In this study, adhering human keratinocytes were investigated for their ability to organize such specialized lipid domains. Successful fluorescent probing of lipid domains, by either inserting exogenous sphingomyelin (BODIPY-SM) or using detoxified fragments of lysenin and theta toxins fused to mCherry, allowed specific, sensitive and quantitative detection of sphingomyelin and cholesterol and demonstrated for the first time submicrometric organization of lipid domains in living keratinocytes. Potential functionality of such domains was additionally assessed during replicative senescence, notably through gradual disappearance of SM-rich domains in senescent keratinocytes. Indeed, SM-rich domains were found critical to preserve keratinocyte migration before senescence, because sphingomyelin or cholesterol depletion in keratinocytes significantly alters lipid domains and reduce migration ability.",
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author = "Abdallah Mound and Vesela Lozanova and C{\'e}line Warnon and Maryse Hermant and Julie Robic and Christelle Guere and Katell Vie and {Lambert de Rouvroit}, Catherine and Donatienne Tyteca and Florence Debacq-Chainiaux and Yves Poumay",
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year = "2017",
month = "6",
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language = "English",
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T1 - Non-senescent keratinocytes organize in plasma membrane submicrometric lipid domains enriched in sphingomyelin and involved in re-epithelialization

AU - Mound, Abdallah

AU - Lozanova, Vesela

AU - Warnon, Céline

AU - Hermant, Maryse

AU - Robic, Julie

AU - Guere, Christelle

AU - Vie, Katell

AU - Lambert de Rouvroit, Catherine

AU - Tyteca, Donatienne

AU - Debacq-Chainiaux, Florence

AU - Poumay, Yves

N1 - Copyright © 2017. Published by Elsevier B.V.

PY - 2017/6/23

Y1 - 2017/6/23

N2 - Membrane lipid raft model has long been debated, but recently the concept of lipid submicrometric domains has emerged to characterize larger (micrometric) and more stable lipid membrane domains. Such domains organize signaling platforms involved in normal or pathological conditions. In this study, adhering human keratinocytes were investigated for their ability to organize such specialized lipid domains. Successful fluorescent probing of lipid domains, by either inserting exogenous sphingomyelin (BODIPY-SM) or using detoxified fragments of lysenin and theta toxins fused to mCherry, allowed specific, sensitive and quantitative detection of sphingomyelin and cholesterol and demonstrated for the first time submicrometric organization of lipid domains in living keratinocytes. Potential functionality of such domains was additionally assessed during replicative senescence, notably through gradual disappearance of SM-rich domains in senescent keratinocytes. Indeed, SM-rich domains were found critical to preserve keratinocyte migration before senescence, because sphingomyelin or cholesterol depletion in keratinocytes significantly alters lipid domains and reduce migration ability.

AB - Membrane lipid raft model has long been debated, but recently the concept of lipid submicrometric domains has emerged to characterize larger (micrometric) and more stable lipid membrane domains. Such domains organize signaling platforms involved in normal or pathological conditions. In this study, adhering human keratinocytes were investigated for their ability to organize such specialized lipid domains. Successful fluorescent probing of lipid domains, by either inserting exogenous sphingomyelin (BODIPY-SM) or using detoxified fragments of lysenin and theta toxins fused to mCherry, allowed specific, sensitive and quantitative detection of sphingomyelin and cholesterol and demonstrated for the first time submicrometric organization of lipid domains in living keratinocytes. Potential functionality of such domains was additionally assessed during replicative senescence, notably through gradual disappearance of SM-rich domains in senescent keratinocytes. Indeed, SM-rich domains were found critical to preserve keratinocyte migration before senescence, because sphingomyelin or cholesterol depletion in keratinocytes significantly alters lipid domains and reduce migration ability.

KW - Journal Article

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KW - Lipid submicrometric domains

KW - Keratinocytes

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KW - Cholesterol

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KW - Lipids/physiology

KW - Membrane Microdomains/metabolism

KW - Cholesterol/metabolism

KW - Humans

KW - Cells, Cultured

KW - Keratinocytes/metabolism

KW - Cell Movement/physiology

KW - Cell Membrane/metabolism

KW - Re-Epithelialization/physiology

KW - Membrane Lipids/metabolism

KW - Toxins, Biological/metabolism

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M3 - Article

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VL - 1862

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JO - Biochimica Biophysica Acta.

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