Non-senescent keratinocytes organize in plasma membrane submicrometric lipid domains enriched in sphingomyelin and involved in re-epithelialization

Abdallah Mound, Vesela Lozanova, Céline Warnon, Maryse Hermant, Julie Robic, Christelle Guere, Katell Vie, Catherine Lambert de Rouvroit, Donatienne Tyteca, Florence Debacq-Chainiaux, Yves Poumay

Research output: Contribution to journalArticle

Abstract

Membrane lipid raft model has long been debated, but recently the concept of lipid submicrometric domains has emerged to characterize larger (micrometric) and more stable lipid membrane domains. Such domains organize signaling platforms involved in normal or pathological conditions. In this study, adhering human keratinocytes were investigated for their ability to organize such specialized lipid domains. Successful fluorescent probing of lipid domains, by either inserting exogenous sphingomyelin (BODIPY-SM) or using detoxified fragments of lysenin and theta toxins fused to mCherry, allowed specific, sensitive and quantitative detection of sphingomyelin and cholesterol and demonstrated for the first time submicrometric organization of lipid domains in living keratinocytes. Potential functionality of such domains was additionally assessed during replicative senescence, notably through gradual disappearance of SM-rich domains in senescent keratinocytes. Indeed, SM-rich domains were found critical to preserve keratinocyte migration before senescence, because sphingomyelin or cholesterol depletion in keratinocytes significantly alters lipid domains and reduce migration ability.

Original languageEnglish
Pages (from-to)958-971
Number of pages14
JournalBiochimica Biophysica Acta.
Volume1862
Issue number9
DOIs
Publication statusPublished - 23 Jun 2017

Keywords

  • Journal Article
  • Senescence
  • Lipid submicrometric domains
  • Keratinocytes
  • Sphingomyelin
  • Keratinocyte migration
  • Cholesterol
  • Sphingomyelins/metabolism
  • Lipids/physiology
  • Membrane Microdomains/metabolism
  • Cholesterol/metabolism
  • Humans
  • Cells, Cultured
  • Keratinocytes/metabolism
  • Cell Movement/physiology
  • Cell Membrane/metabolism
  • Re-Epithelialization/physiology
  • Membrane Lipids/metabolism
  • Toxins, Biological/metabolism

Fingerprint Dive into the research topics of 'Non-senescent keratinocytes organize in plasma membrane submicrometric lipid domains enriched in sphingomyelin and involved in re-epithelialization'. Together they form a unique fingerprint.

  • Equipment

    Morphology - Imaging

    Charles Nicaise (Manager) & Francesca Cecchet (Manager)

    Technological Platform Morphology - Imaging

    Facility/equipment: Technological Platform

  • Activities

    • 1 External Thesis

    Cite this