Multi-walled carbon nanotube/poly(glycine) modified carbon paste electrode for the determination of dopamine in biological fluids and pharmaceuticals

Tony Thomas, Ronald J. Mascarenhas, B. E Kumara Swamy, Praveen Martis, Zineb Mekhalif, B. S. Sherigara

Research output: Contribution to journalArticle

Abstract

A modified carbon paste electrode (CPE) for the selective detection of dopamine (DA) in presence of large excess of ascorbic acid (AA) and uric acid (UA) at physiological pH has been fabricated by bulk modification of CPE with multi-walled carbon nanotubes (MWCNTs) followed by electropolymerization of glycine (Gly). The surface morphology is compared using SEM images. The presence of nitrogen was confirmed by the energy dispersion X-ray spectroscopy (EDS) indicating the polymerization of Gly on the surface of the modified electrode. The impedance study indicates a better charge transfer kinetics for DA at CPE modified with MWCNT/polyglycine electrode. The presence of MWCNTs in carbon paste matrix triggers the extent of electropolymerization of Gly and imparts more selectivity towards DA by electrochemically not sensing AA below a concentration of 3.1×10-4M. Due to the exclusion of the signal for AA, the interference of AA in the determination of DA is totally ruled out by DPV method which is used for its detection at lower concentrations. Large peak separation, good sensitivity, reproducibility and stability allow this modified electrode to analyze DA individually and simultaneously along with AA and UA. Detection limit of DA was determined from differential pulse voltammetric (DPV) study and found to be 1.2×10-8M with a linear dynamic range of 5.0×10-7M to 4.0×10-5M. The practical analytical application of this electrode was demonstrated by measurement of DA content in dopamine hydrochloride injection and human blood serum.

Original languageEnglish
Pages (from-to)458-465
Number of pages8
JournalColloids and Surfaces B: Biointerfaces
Volume110
DOIs
Publication statusPublished - 1 Oct 2013

Fingerprint

dopamine
Carbon Nanotubes
Ointments
glycine
Drug products
Glycine
Amino acids
Dopamine
Carbon nanotubes
Electrodes
Carbon
carbon nanotubes
ascorbic acid
Ascorbic acid
Fluids
electrodes
Ascorbic Acid
carbon
fluids
Pharmaceutical Preparations

Keywords

  • Carbon paste electrode
  • Dopamine
  • Electropolymerization
  • Glycine
  • Multi-walled carbon nanotube

Cite this

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title = "Multi-walled carbon nanotube/poly(glycine) modified carbon paste electrode for the determination of dopamine in biological fluids and pharmaceuticals",
abstract = "A modified carbon paste electrode (CPE) for the selective detection of dopamine (DA) in presence of large excess of ascorbic acid (AA) and uric acid (UA) at physiological pH has been fabricated by bulk modification of CPE with multi-walled carbon nanotubes (MWCNTs) followed by electropolymerization of glycine (Gly). The surface morphology is compared using SEM images. The presence of nitrogen was confirmed by the energy dispersion X-ray spectroscopy (EDS) indicating the polymerization of Gly on the surface of the modified electrode. The impedance study indicates a better charge transfer kinetics for DA at CPE modified with MWCNT/polyglycine electrode. The presence of MWCNTs in carbon paste matrix triggers the extent of electropolymerization of Gly and imparts more selectivity towards DA by electrochemically not sensing AA below a concentration of 3.1×10-4M. Due to the exclusion of the signal for AA, the interference of AA in the determination of DA is totally ruled out by DPV method which is used for its detection at lower concentrations. Large peak separation, good sensitivity, reproducibility and stability allow this modified electrode to analyze DA individually and simultaneously along with AA and UA. Detection limit of DA was determined from differential pulse voltammetric (DPV) study and found to be 1.2×10-8M with a linear dynamic range of 5.0×10-7M to 4.0×10-5M. The practical analytical application of this electrode was demonstrated by measurement of DA content in dopamine hydrochloride injection and human blood serum.",
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Multi-walled carbon nanotube/poly(glycine) modified carbon paste electrode for the determination of dopamine in biological fluids and pharmaceuticals. / Thomas, Tony; Mascarenhas, Ronald J.; Swamy, B. E Kumara; Martis, Praveen; Mekhalif, Zineb; Sherigara, B. S.

In: Colloids and Surfaces B: Biointerfaces, Vol. 110, 01.10.2013, p. 458-465.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Multi-walled carbon nanotube/poly(glycine) modified carbon paste electrode for the determination of dopamine in biological fluids and pharmaceuticals

AU - Thomas, Tony

AU - Mascarenhas, Ronald J.

AU - Swamy, B. E Kumara

AU - Martis, Praveen

AU - Mekhalif, Zineb

AU - Sherigara, B. S.

PY - 2013/10/1

Y1 - 2013/10/1

N2 - A modified carbon paste electrode (CPE) for the selective detection of dopamine (DA) in presence of large excess of ascorbic acid (AA) and uric acid (UA) at physiological pH has been fabricated by bulk modification of CPE with multi-walled carbon nanotubes (MWCNTs) followed by electropolymerization of glycine (Gly). The surface morphology is compared using SEM images. The presence of nitrogen was confirmed by the energy dispersion X-ray spectroscopy (EDS) indicating the polymerization of Gly on the surface of the modified electrode. The impedance study indicates a better charge transfer kinetics for DA at CPE modified with MWCNT/polyglycine electrode. The presence of MWCNTs in carbon paste matrix triggers the extent of electropolymerization of Gly and imparts more selectivity towards DA by electrochemically not sensing AA below a concentration of 3.1×10-4M. Due to the exclusion of the signal for AA, the interference of AA in the determination of DA is totally ruled out by DPV method which is used for its detection at lower concentrations. Large peak separation, good sensitivity, reproducibility and stability allow this modified electrode to analyze DA individually and simultaneously along with AA and UA. Detection limit of DA was determined from differential pulse voltammetric (DPV) study and found to be 1.2×10-8M with a linear dynamic range of 5.0×10-7M to 4.0×10-5M. The practical analytical application of this electrode was demonstrated by measurement of DA content in dopamine hydrochloride injection and human blood serum.

AB - A modified carbon paste electrode (CPE) for the selective detection of dopamine (DA) in presence of large excess of ascorbic acid (AA) and uric acid (UA) at physiological pH has been fabricated by bulk modification of CPE with multi-walled carbon nanotubes (MWCNTs) followed by electropolymerization of glycine (Gly). The surface morphology is compared using SEM images. The presence of nitrogen was confirmed by the energy dispersion X-ray spectroscopy (EDS) indicating the polymerization of Gly on the surface of the modified electrode. The impedance study indicates a better charge transfer kinetics for DA at CPE modified with MWCNT/polyglycine electrode. The presence of MWCNTs in carbon paste matrix triggers the extent of electropolymerization of Gly and imparts more selectivity towards DA by electrochemically not sensing AA below a concentration of 3.1×10-4M. Due to the exclusion of the signal for AA, the interference of AA in the determination of DA is totally ruled out by DPV method which is used for its detection at lower concentrations. Large peak separation, good sensitivity, reproducibility and stability allow this modified electrode to analyze DA individually and simultaneously along with AA and UA. Detection limit of DA was determined from differential pulse voltammetric (DPV) study and found to be 1.2×10-8M with a linear dynamic range of 5.0×10-7M to 4.0×10-5M. The practical analytical application of this electrode was demonstrated by measurement of DA content in dopamine hydrochloride injection and human blood serum.

KW - Carbon paste electrode

KW - Dopamine

KW - Electropolymerization

KW - Glycine

KW - Multi-walled carbon nanotube

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DO - 10.1016/j.colsurfb.2013.03.056

M3 - Article

VL - 110

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EP - 465

JO - Colloids and Surfaces B: Biointerfaces

JF - Colloids and Surfaces B: Biointerfaces

SN - 0927-7765

ER -