TY - JOUR
T1 - Molecular insights into Vibrio cholerae's intra-amoebal host-pathogen interactions
AU - Van der Henst, Charles
AU - Vanhove, Audrey Sophie
AU - Drebes Dörr, Natália Carolina
AU - Stutzmann, Sandrine
AU - Stoudmann, Candice
AU - Clerc, Stéphanie
AU - Scrignari, Tiziana
AU - Maclachlan, Catherine
AU - Knott, Graham
AU - Blokesch, Melanie
N1 - Funding Information:
We thank members of the Blokesch group at EPFL and the Geneva/Lausanne amoeba club for fruitful discussions. We also acknowledge J. Mekalanos (Harvard) for strain C6706 (original stock) and former members of the Blokesch lab for provision of genetically engineered V. cholerae strains. This work was supported by EPFL intramural funding, a Starting Grant from the European Research Council (ERC; 309064-VIR4-ENV), and a Consolidator Grant from the European Research Council (ERC; 724630-CholeraIndex) to MB. M.B. is a Howard Hughes Medical Institute (HHMI) International Research Scholar (grant 55008726).
Publisher Copyright:
© 2018, The Author(s).
Copyright:
Copyright 2018 Elsevier B.V., All rights reserved.
PY - 2018/12/1
Y1 - 2018/12/1
N2 - Vibrio cholerae, which causes the diarrheal disease cholera, is a species of bacteria commonly found in aquatic habitats. Within such environments, the bacterium must defend itself against predatory protozoan grazers. Amoebae are prominent grazers, with Acanthamoeba castellanii being one of the best-studied aquatic amoebae. We previously showed that V. cholerae resists digestion by A. castellanii and establishes a replication niche within the host's osmoregulatory organelle. In this study, we decipher the molecular mechanisms involved in the maintenance of V. cholerae's intra-amoebal replication niche and its ultimate escape from the succumbed host. We demonstrate that minor virulence features important for disease in mammals, such as extracellular enzymes and flagellum-based motility, have a key role in the replication and transmission of V. cholerae in its aqueous environment. This work, therefore, describes new mechanisms that provide the pathogen with a fitness advantage in its primary habitat, which may have contributed to the emergence of these minor virulence factors in the species V. cholerae.
AB - Vibrio cholerae, which causes the diarrheal disease cholera, is a species of bacteria commonly found in aquatic habitats. Within such environments, the bacterium must defend itself against predatory protozoan grazers. Amoebae are prominent grazers, with Acanthamoeba castellanii being one of the best-studied aquatic amoebae. We previously showed that V. cholerae resists digestion by A. castellanii and establishes a replication niche within the host's osmoregulatory organelle. In this study, we decipher the molecular mechanisms involved in the maintenance of V. cholerae's intra-amoebal replication niche and its ultimate escape from the succumbed host. We demonstrate that minor virulence features important for disease in mammals, such as extracellular enzymes and flagellum-based motility, have a key role in the replication and transmission of V. cholerae in its aqueous environment. This work, therefore, describes new mechanisms that provide the pathogen with a fitness advantage in its primary habitat, which may have contributed to the emergence of these minor virulence factors in the species V. cholerae.
UR - http://www.scopus.com/inward/record.url?scp=85052285072&partnerID=8YFLogxK
U2 - 10.1038/s41467-018-05976-x
DO - 10.1038/s41467-018-05976-x
M3 - Article
C2 - 30150745
SN - 2041-1723
VL - 9
SP - 3460
JO - Nature Communications
JF - Nature Communications
IS - 1
M1 - 3460
ER -