TY - JOUR
T1 - Modulation of plant plasma membrane structure by exogenous fatty acid hydroperoxide is a potential perception mechanism for their eliciting activity
AU - Deboever, Estelle
AU - Van Aubel, Géraldine
AU - Rondelli, Valeria
AU - Koutsioubas, Alexandros
AU - Mathelie-Guinlet, Marion
AU - Dufrene, Yves F.
AU - Ongena, Marc
AU - Lins, Laurence
AU - Van Cutsem, Pierre
AU - Fauconnier, Marie Laure
AU - Deleu, Magali
N1 - Funding Information:
The authors thank the financial support via the project from the University of Liège (ARC‐FIELD project 13/17‐10). Authors also thank beamline MARIA Jülich Centre for Neutron Science (JCNS) at Heinz Maier‐Leibnitz Zentrum (MLZ, Garching, Germany) for allocation of beamtime. Acknowledgements are also due to FytoFend's research team for their logistic support in bioassays. Final thanks to Jelena Prisc for technical support and nice discussion on ISR and ROS experiment data measured . E.D. is supported by a « Fonds pour la formation à la Recherche dans l'Industrie et dans l'Agriculture » (FRIA) grant (5100617 F) from the FRS‐FNRS (Fonds National de la Recherche Scientifique, Belgium). M.D., M. O. and L.L. thank the FRS‐FNRS for their position as Senior Research Associates and for grant CDR (J.0014.08 and J.0086.18 projects). Work at the Université Catholique de Louvain was supported by the National Fund for Scientific Research (FNRS) and the Research Department of the Communauté Française de Belgique (Concerted Research Action). Y.F.D. is a Research Director at the FNRS. This study was funded also by the ‘Medical Biotechnologies and Translational Medicine Department’ of the ‘Università degli Studi di Milano’, grant number ‘PSR2018’ to V.R. This article was published with the support of the ‘Fondation Universitaire de Belgique’. in planta
Publisher Copyright:
© 2021 John Wiley & Sons Ltd.
PY - 2021/12/3
Y1 - 2021/12/3
N2 - Oxylipins are lipid-derived molecules that are ubiquitous in eukaryotes and whose functions in plant physiology have been widely reported. They appear to play a major role in plant immunity by orchestrating reactive oxygen species (ROS) and hormone-dependent signalling pathways. The present work focuses on the specific case of fatty acid hydroperoxides (HPOs). Although some studies report their potential use as exogenous biocontrol agents for plant protection, evaluation of their efficiency in planta is lacking and no information is available about their mechanism of action. In this study, the potential of 13(S)-hydroperoxy-(9Z, 11E)-octadecadienoic acid (13-HPOD) and 13(S)-hydroperoxy-(9Z, 11E, 15Z)-octadecatrienoic acid (13-HPOT), as plant defence elicitors and the underlying mechanism of action is investigated. Arabidopsis thaliana leaf resistance to Botrytis cinerea was observed after root application with HPOs. They also activate early immunity-related defence responses, like ROS. As previous studies have demonstrated their ability to interact with plant plasma membranes (PPM), we have further investigated the effects of HPOs on biomimetic PPM structure using complementary biophysics tools. Results show that HPO insertion into PPM impacts its global structure without solubilizing it. The relationship between biological assays and biophysical analysis suggests that lipid amphiphilic elicitors that directly act on membrane lipids might trigger early plant defence events.
AB - Oxylipins are lipid-derived molecules that are ubiquitous in eukaryotes and whose functions in plant physiology have been widely reported. They appear to play a major role in plant immunity by orchestrating reactive oxygen species (ROS) and hormone-dependent signalling pathways. The present work focuses on the specific case of fatty acid hydroperoxides (HPOs). Although some studies report their potential use as exogenous biocontrol agents for plant protection, evaluation of their efficiency in planta is lacking and no information is available about their mechanism of action. In this study, the potential of 13(S)-hydroperoxy-(9Z, 11E)-octadecadienoic acid (13-HPOD) and 13(S)-hydroperoxy-(9Z, 11E, 15Z)-octadecatrienoic acid (13-HPOT), as plant defence elicitors and the underlying mechanism of action is investigated. Arabidopsis thaliana leaf resistance to Botrytis cinerea was observed after root application with HPOs. They also activate early immunity-related defence responses, like ROS. As previous studies have demonstrated their ability to interact with plant plasma membranes (PPM), we have further investigated the effects of HPOs on biomimetic PPM structure using complementary biophysics tools. Results show that HPO insertion into PPM impacts its global structure without solubilizing it. The relationship between biological assays and biophysical analysis suggests that lipid amphiphilic elicitors that directly act on membrane lipids might trigger early plant defence events.
KW - elicitor
KW - fatty acid hydroperoxide
KW - molecular mechanism
KW - oxidative burst
KW - oxylipin
KW - plant defence
KW - plant plasma membrane
UR - http://www.scopus.com/inward/record.url?scp=85122768995&partnerID=8YFLogxK
U2 - 10.1111/pce.14239
DO - 10.1111/pce.14239
M3 - Article
AN - SCOPUS:85122768995
SN - 0140-7791
VL - 45
SP - 1082
EP - 1095
JO - Plant Cell and Environment
JF - Plant Cell and Environment
IS - 4
ER -