Projects per year
Abstract
In adipocytes, mitochondrial uncoupling is known to trigger a triglyceride loss comparable with the one induced by TNFα, a proinflammatory cytokine. However, the impact of a mitochondrial uncoupling on the abundance/composition of mitochondria and its connection with triglyceride content in adipocytes is largely unknown. In this work, the effects of a mild mitochondrial uncoupling triggered by FCCP were investigated on the mitochondrial population of 3T3-L1 adipocytes by both quantitative and qualitative approaches. We found that mild mitochondrial uncoupling does not stimulate mitochondrial biogenesis in adipocytes but induces an adaptive cell response characterized by quantitative modifications of mitochondrial protein content. Superoxide anion radical level was increased in mitochondria of both TNFα- and FCCP-treated adipocytes, whereas mitochondrial DNA copy number was significantly higher only in TNFα-treated cells. Subproteomic analysis revealed that the abundance of pyruvate carboxylase was reduced significantly in mitochondria of TNFα- and FCCP-treated adipocytes. Functional study showed that overexpres-sion of this major enzyme of lipid metabolism is able to prevent the triglyceride content reduction in adipocytes exposed to mitochondrial uncoupling or TNFα. These results suggest a new mechanism by which the effects of mitochondrial uncoupling might limit triglyceride accumulation in adipocytes.
Original language | English |
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Pages (from-to) | E1123-41 |
Number of pages | 19 |
Journal | American Journal of Physiology - Endocrinology and Metabolism |
Volume | 302 |
Issue number | 9 |
DOIs | |
Publication status | Published - 1 May 2012 |
Fingerprint Dive into the research topics of 'Mild mitochondrial uncoupling does not affect mitochondrial biogenesis but downregulates pyruvate carboxylase in adipocytes: Role for triglyceride content reduction'. Together they form a unique fingerprint.
Projects
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Study of the mechanisms involved in the adipocyte dedifferentiation induced by mitochondrial uncoupling : role of mitochondrial biogenesis
ARNOULD, T. & DE PAUW, A.
1/10/05 → 31/01/11
Project: PHD
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Study of cell responses to mitochondrial dysfunction: implications in the maintenance of mitochondrial biogenesis and apoptosis
ARNOULD, T., Renard, P. & MERCY, L.
1/10/02 → 31/03/08
Project: PHD
Equipment
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Confocal microscope
Charles Nicaise (Manager) & Francesca Cecchet (Manager)
Technological Platform Morphology - ImagingFacility/equipment: Equipment
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Mass Spectrometry Service
Patricia Renard (Manager)
Technological Platform Mass Spectrometry ServiceFacility/equipment: Technological Platform
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Transmission electron microscope
Charles Nicaise (Manager) & Francesca Cecchet (Manager)
Technological Platform Morphology - ImagingFacility/equipment: Equipment
Student Theses
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Mise en évidence du rôle de CREB dans l'expression différentielle de gènes induite par l'inhibition mitochondriale dans des préadipocytes 3T3-L1
Author: De Pauw, A., 2004Supervisor: Arnould, T. (Supervisor)
Student thesis: Master types › Master in Biology
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