TY - JOUR
T1 - Microplastics alter development, behavior, and innate immunity responses following bacterial infection during zebrafish embryo-larval development
AU - Rabezanahary, Andry Ny Aina
AU - Piette, Mathilde
AU - Missawi, Omayma
AU - Garigliany, Mutien Marie
AU - Kestemont, Patrick
AU - Cornet, Valérie
N1 - Funding Information:
The authors thank the Fonds de la Recherche Scientifique ( FNRS ) for their financial support (PDR N°40003703 ). We also thank Marie Guissard, Guillaume Binard, and Noémie Madarasz for their help in the realization of preliminary in vivo experiments.
Publisher Copyright:
© 2022 Elsevier Ltd
PY - 2023/1
Y1 - 2023/1
N2 - Although the hazards of microplastics (MPs) have been explored, no complete data exists on the effect of MPs on the egg chorion. This study aims to evaluate the modification of immune responses, metabolism, and behavior of zebrafish larvae (Danio rerio) depending on the moment of exposure. Larvae were exposed to 5 μm polystyrene microbeads at a concentration of 0, 100, or 1000 μg/l, according to a specified times of exposure (0–4, 4–8, 0–8 days postfertilization (dpf)), followed by a bacterial challenge at 8 dpf. After every 4 and 8 dpf, swimming activity, gene expression related to oxidative stress and immune system responses were assessed. During embryonic development, larvae exposed to a concentration of 1000 μg/l MPs already showed a significantly reduced tail coiling frequency, yolk sac resorption and heartbeat. At 8 dpf, swimming activity was altered, even without ingestion and a few days after the end of MP exposure. Our results indicated a difference in immune system (nfkb, il1β) and apoptosis (casp3a, bcl2) related gene expression depending on the timing of MP exposure, which highlighted a contrasting sensitivity according to the exposure time in MP studies. This study brings new insight into how MPs might affect zebrafish larvae health and development even without ingestion.
AB - Although the hazards of microplastics (MPs) have been explored, no complete data exists on the effect of MPs on the egg chorion. This study aims to evaluate the modification of immune responses, metabolism, and behavior of zebrafish larvae (Danio rerio) depending on the moment of exposure. Larvae were exposed to 5 μm polystyrene microbeads at a concentration of 0, 100, or 1000 μg/l, according to a specified times of exposure (0–4, 4–8, 0–8 days postfertilization (dpf)), followed by a bacterial challenge at 8 dpf. After every 4 and 8 dpf, swimming activity, gene expression related to oxidative stress and immune system responses were assessed. During embryonic development, larvae exposed to a concentration of 1000 μg/l MPs already showed a significantly reduced tail coiling frequency, yolk sac resorption and heartbeat. At 8 dpf, swimming activity was altered, even without ingestion and a few days after the end of MP exposure. Our results indicated a difference in immune system (nfkb, il1β) and apoptosis (casp3a, bcl2) related gene expression depending on the timing of MP exposure, which highlighted a contrasting sensitivity according to the exposure time in MP studies. This study brings new insight into how MPs might affect zebrafish larvae health and development even without ingestion.
KW - Apoptosis
KW - Bacterial infection
KW - Immunity
KW - Microplastics
KW - Polystyrene microbeads
KW - Zebrafish larvae
UR - http://www.scopus.com/inward/record.url?scp=85140610075&partnerID=8YFLogxK
U2 - 10.1016/j.chemosphere.2022.136969
DO - 10.1016/j.chemosphere.2022.136969
M3 - Article
C2 - 36306963
AN - SCOPUS:85140610075
SN - 0045-6535
VL - 311
JO - Chemosphere
JF - Chemosphere
M1 - 136969
ER -