Low-dose endothelin-1 potentiates volume-induced secretion of atrial natriuretic factor

J. Donckier, C. Hanet, L. Galanti, L. Stoleru, H. Van Mechelen, A. Robert, J. M. Ketelslegers, H. Pouleur

Research output: Contribution to journalArticle

Abstract

To evaluate the role of calcium in volume-induced secretion of atrial natriuretic factor (ANF), effects of verapamil and endothelin-1 (ET-1), both known to influence free intracellular calcium, were studied during saline infusion in seven conscious instrumented dogs. Fifteen minutes after intravenous injection of placebo or verapamil (0.25 mg/kg) or during continuous ET-1 infusion (at 5 ng · kg-1 · min-1), saline (10% body wt) was infused during 40 min. Saline produced a rise (P < 0.001) of plasma ANF from 28.1 ± 6.3 to 50.4 ± 12.9 pM after placebo, from 30.2 ± 6.1 to 51.1 ± 8.5 pM after verapamil, and from 31.2 ± 6.1 to 81.0 ± 12.9 pM with ET-1. This increase was comparable after placebo and verapamil, but was 80% greater with ET-1 (P < 0.02). Plasma ET-1, unchanged after placebo, rose (P < 0.001) from 1.7 ± 0.5 to 38.3 ± 9.2 pM with ET-1. In the three experiments, heart rate and left atrial pressure (LAP) increased (P < 0.001) similarly. The linear relation between ANF and LAP was steeper with ET-1 than with saline or verapamil (both P < 0.05), indicating that the enhanced ANF secretion with ET-1 was occurring at all levels of LAP. Thus volume-induced secretion of ANF is not suppressed by verapamil, but is directly enhanced by low-dose ET-1, known to activate the phosphoinositide pathway.

Original languageEnglish
Pages (from-to)H939-H944
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Volume263
Issue number3 32-3
Publication statusPublished - 1 Jan 1992
Externally publishedYes

Keywords

  • atrial natriuretic peptide
  • atrial pressure
  • atrial stretch
  • calcium channel
  • verapamil

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    Donckier, J., Hanet, C., Galanti, L., Stoleru, L., Van Mechelen, H., Robert, A., Ketelslegers, J. M., & Pouleur, H. (1992). Low-dose endothelin-1 potentiates volume-induced secretion of atrial natriuretic factor. American Journal of Physiology - Heart and Circulatory Physiology, 263(3 32-3), H939-H944.