TY - JOUR
T1 - Low-dose endothelin-1 potentiates volume-induced secretion of atrial natriuretic factor
AU - Donckier, J.
AU - Hanet, C.
AU - Galanti, L.
AU - Stoleru, L.
AU - Van Mechelen, H.
AU - Robert, A.
AU - Ketelslegers, J. M.
AU - Pouleur, H.
PY - 1992/1/1
Y1 - 1992/1/1
N2 - To evaluate the role of calcium in volume-induced secretion of atrial natriuretic factor (ANF), effects of verapamil and endothelin-1 (ET-1), both known to influence free intracellular calcium, were studied during saline infusion in seven conscious instrumented dogs. Fifteen minutes after intravenous injection of placebo or verapamil (0.25 mg/kg) or during continuous ET-1 infusion (at 5 ng · kg-1 · min-1), saline (10% body wt) was infused during 40 min. Saline produced a rise (P < 0.001) of plasma ANF from 28.1 ± 6.3 to 50.4 ± 12.9 pM after placebo, from 30.2 ± 6.1 to 51.1 ± 8.5 pM after verapamil, and from 31.2 ± 6.1 to 81.0 ± 12.9 pM with ET-1. This increase was comparable after placebo and verapamil, but was 80% greater with ET-1 (P < 0.02). Plasma ET-1, unchanged after placebo, rose (P < 0.001) from 1.7 ± 0.5 to 38.3 ± 9.2 pM with ET-1. In the three experiments, heart rate and left atrial pressure (LAP) increased (P < 0.001) similarly. The linear relation between ANF and LAP was steeper with ET-1 than with saline or verapamil (both P < 0.05), indicating that the enhanced ANF secretion with ET-1 was occurring at all levels of LAP. Thus volume-induced secretion of ANF is not suppressed by verapamil, but is directly enhanced by low-dose ET-1, known to activate the phosphoinositide pathway.
AB - To evaluate the role of calcium in volume-induced secretion of atrial natriuretic factor (ANF), effects of verapamil and endothelin-1 (ET-1), both known to influence free intracellular calcium, were studied during saline infusion in seven conscious instrumented dogs. Fifteen minutes after intravenous injection of placebo or verapamil (0.25 mg/kg) or during continuous ET-1 infusion (at 5 ng · kg-1 · min-1), saline (10% body wt) was infused during 40 min. Saline produced a rise (P < 0.001) of plasma ANF from 28.1 ± 6.3 to 50.4 ± 12.9 pM after placebo, from 30.2 ± 6.1 to 51.1 ± 8.5 pM after verapamil, and from 31.2 ± 6.1 to 81.0 ± 12.9 pM with ET-1. This increase was comparable after placebo and verapamil, but was 80% greater with ET-1 (P < 0.02). Plasma ET-1, unchanged after placebo, rose (P < 0.001) from 1.7 ± 0.5 to 38.3 ± 9.2 pM with ET-1. In the three experiments, heart rate and left atrial pressure (LAP) increased (P < 0.001) similarly. The linear relation between ANF and LAP was steeper with ET-1 than with saline or verapamil (both P < 0.05), indicating that the enhanced ANF secretion with ET-1 was occurring at all levels of LAP. Thus volume-induced secretion of ANF is not suppressed by verapamil, but is directly enhanced by low-dose ET-1, known to activate the phosphoinositide pathway.
KW - atrial natriuretic peptide
KW - atrial pressure
KW - atrial stretch
KW - calcium channel
KW - verapamil
UR - http://www.scopus.com/inward/record.url?scp=0026778552&partnerID=8YFLogxK
M3 - Article
C2 - 1415620
AN - SCOPUS:0026778552
SN - 0002-9513
VL - 263
SP - H939-H944
JO - American Journal of Physiology - Heart and Circulatory Physiology
JF - American Journal of Physiology - Heart and Circulatory Physiology
IS - 3 32-3
ER -