Localized incorporation of outer membrane components in the pathogen Brucella abortus

Victoria Vassen, Claire Valotteau, Cécile Feuillie, Cécile Formosa-Dague, Yves F Dufrêne, Xavier De Bolle

Research output: Contribution to journalArticle

Abstract

The zoonotic pathogen Brucella abortus is part of the Rhizobiales, which are alpha-proteobacteria displaying unipolar growth. Here, we show that this bacterium exhibits heterogeneity in its outer membrane composition, with clusters of rough lipopolysaccharide co-localizing with the essential outer membrane porin Omp2b, which is proposed to allow facilitated diffusion of solutes through the porin. We also show that the major outer membrane protein Omp25 and peptidoglycan are incorporated at the new pole and the division site, the expected growth sites. Interestingly, lipopolysaccharide is also inserted at the same growth sites. The absence of long-range diffusion of main components of the outer membrane could explain the apparent immobility of the Omp2b clusters, as well as unipolar and mid-cell localizations of newly incorporated outer membrane proteins and lipopolysaccharide. Unipolar growth and limited mobility of surface structures also suggest that new surface variants could arise in a few generations without the need of diluting pre-existing surface antigens.

Original languageEnglish
Article numbere100323
JournalThe EMBO journal
Volume38
Issue number5
Early online date2019
DOIs
Publication statusPublished - 1 Mar 2019

Fingerprint

Brucella abortus
Pathogens
Membranes
Lipopolysaccharides
Porins
Growth
Membrane Proteins
Facilitated Diffusion
Alphaproteobacteria
Peptidoglycan
Zoonoses
Surface Antigens
Surface structure
Poles
Bacteria
Chemical analysis

Keywords

  • Brucella
  • lipopolysaccharide
  • outer membrane
  • unipolar growth

Cite this

Vassen, Victoria ; Valotteau, Claire ; Feuillie, Cécile ; Formosa-Dague, Cécile ; Dufrêne, Yves F ; De Bolle, Xavier. / Localized incorporation of outer membrane components in the pathogen Brucella abortus. In: The EMBO journal. 2019 ; Vol. 38, No. 5.
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Localized incorporation of outer membrane components in the pathogen Brucella abortus. / Vassen, Victoria; Valotteau, Claire; Feuillie, Cécile; Formosa-Dague, Cécile; Dufrêne, Yves F; De Bolle, Xavier.

In: The EMBO journal, Vol. 38, No. 5, e100323, 01.03.2019.

Research output: Contribution to journalArticle

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AU - Feuillie, Cécile

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