Projects per year
Lipopolysaccharide is essential for most Gram-negative bacteria as it is a main component of the outer membrane. In the pathogen Brucella abortus, smooth lipopolysaccharide containing the O-antigen is required for virulence. Being part of the Rhizobiales, Brucella spp. display unipolar growth and lipopolysaccharide was shown to be incorporated at the active growth sites, i.e. the new pole and the division site. By localizing proteins involved in the lipopolysaccharide transport across the cell envelope, from the inner to the outer membrane, we show that the lipopolysaccharide incorporation sites are determined by the inner membrane complex of the lipopolysaccharide transport system. Moreover, we identify the main O-antigen ligase of Brucella spp. involved in smooth lipopolysaccharide synthesis. Altogether, our data highlight a layer of spatiotemporal organization of the lipopolysaccharide biosynthesis pathway and identify an original class of bifunctional O-antigen ligases.
|Publication status||Published - 17 Feb 2023|
- Brucella abortus/genetics
- O Antigens
- Carbohydrate Metabolism
- Cell Membrane
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- 2 Active
Single cell analysis of Brucella growth
1/01/20 → 31/12/23
PDR (FRSM) BRUCELL-CYCLE: BRUCELL-CYCLE
De Bolle, X., Willemart, K. & ONG, T. A. P.
31/12/14 → …
Biological Security Laboratory Level 3 (BL3)
Xavier De Bolle (Manager)Technological Platform: Biological Security Laboratory Level 3
Facility/equipment: Technological Platform
Smooth lipopolysaccharide biosynthesis, unipolar growth and disulfide bond formation in Brucella abortus cell envelopeAuthor: Servais, C., 27 Feb 2023
Supervisor: De Bolle, X. (Supervisor), Renzi, F. (President), Conde-Álvarez, R. (External person) (Jury), Polissi, A. (External person) (Jury) & Deghelt, M. (External person) (Jury)
Student thesis: Doc types › Doctor of Sciences