Projects per year
Abstract
The development of new modalities and protocols is of major interest to improve the outcome of cancer treatment. Given the appealing physical properties of protons and the emerging evidence of biological relevance of the use of gold nanoparticles (GNPs), the radiosensitization effects of GNPs (5 or 10 nm) have been investigated in vitro in combination with a proton beam of different linear energy transfer (LET). After the incubation with GNPs for 24 h, nanoparticles were observed in the cytoplasm of A431 cells exposed to 10 nm GNPs, and in the cytoplasm as well as the nucleus of cells exposed to 5 nm GNPs. Cell uptake of 0.05 mg ml-1 of GNPs led to 0.78 pg Au/cell and 0.30 pg Au/cell after 24 h incubation for 10 and 5 nm GNPs respectively. A marked radiosensitization effect of GNPs was observed with 25 keV μm-1 protons, but not with 10 keV μm-1 protons. This effect was more pronounced for 10 nm GNPs than for 5 nm GNPs. By using a radical scavenger, a major role of reactive oxygen species in the amplification of the death of irradiated cell was identified. All together, these results open up novel perspectives for using high-Z metallic NPs in protontherapy.
Original language | English |
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Article number | 455101 |
Journal | Nanotechnology |
Volume | 27 |
Issue number | 45 |
DOIs | |
Publication status | Published - 2016 |
Keywords
- A431 cells
- cell uptake
- gold nanoparticles
- proton irradiation
- radiosensitization
Fingerprint
Dive into the research topics of 'LET-dependent radiosensitization effects of gold nanoparticles for proton irradiation'. Together they form a unique fingerprint.Projects
- 1 Finished
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THERAPLUS: Theraplus
Heuskin, A., Lucas, S., MICHIELS, C., Feron, O., Gallez, B. & MARTINIVE, P.
1/05/14 → 1/05/17
Project: Research
Equipment
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Confocal microscope
Charles Nicaise (Manager) & Francesca Cecchet (Manager)
Technological Platform Morphology - ImagingFacility/equipment: Equipment
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Morphology - Imaging
Charles Nicaise (Manager) & Francesca Cecchet (Manager)
Technological Platform Morphology - ImagingFacility/equipment: Technological Platform
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Synthesis, Irradiation and Analysis of Materials (SIAM)
Pierre Louette (Manager), Julien Colaux (Manager), Alexandre Felten (Manager) & Jorge Humberto Mejia Mendoza (Manager)
Technological Platform Synthesis, Irradiation and Analysis of MaterialsFacility/equipment: Technological Platform
Activities
- 1 Participation in conference
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63rd annual Congress of the Radiation Research Society
Stéphane Lucas (Poster)
15 Oct 2017 → 18 Oct 2017Activity: Participating in or organising an event types › Participation in conference