Large-pore membrane hemofiltration increases cytokine clearance and improves right ventricular-vascular coupling during endotoxic shock in pigs

Bernard Lambermont, Pierre Delanaye, Jean-Michel Dogné, Alexandre Ghuysen, Nathalie Janssen, Bernard Dubois, Thomas Desaive, Philippe Kolh, Vincent D'Orio, Jean-Marie Krzesinski

Research output: Contribution to journalArticle

Abstract

Hemodynamic improvement in patients suffering from both septic shock and renal failure who received hemofiltration suggested that an extrarenal epuration technique could be of interest in patients with septic shock alone. However, most of the studies using continuous venovenous hemofiltration (CVVH) in this setting evidenced neither cytokine clearance nor significant reduction in their plasma level. Lack of significant clearance was explained in part by the small size of the membrane pores. Therefore, we investigated the effects of large-pore membrane hemofiltration (LPHF) during endotoxic shock in pigs on interleukin 6 (IL-6) and interleukin 10 (IL-10) clearances, and on right ventricular (RV)-vascular coupling. Thirteen anesthetized healthy pigs weighing 20-30 kg were divided into two groups. In the Endo group (n = 6), the pigs received a 0.5-mg/kg endotoxin infusion over a period of 30 mins from T0 to T30. In the EndoHF group (n = 7), LPHF (cutoff = 80 kDa) and an ultrafiltration rate of 45 mL/kg/h were started 30 mins after the end of the endotoxin infusion, from T60 to T240. In this model of porcine endotoxic shock, LPHF was responsible for a significant clearance of IL-6 (20 mL/min) and Il-10 (14 mL/min), and for an improvement in RV-vascular coupling.
Original languageEnglish
Pages (from-to)560-4
Number of pages5
JournalArtificial organs
Volume30
Issue number7
DOIs
Publication statusPublished - Jul 2006

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Keywords

  • Animals
  • Coronary Vessels
  • Cytokines
  • Hemofiltration
  • Shock, Septic
  • Swine
  • Ventricular Function

Cite this

Lambermont, B., Delanaye, P., Dogné, J-M., Ghuysen, A., Janssen, N., Dubois, B., Desaive, T., Kolh, P., D'Orio, V., & Krzesinski, J-M. (2006). Large-pore membrane hemofiltration increases cytokine clearance and improves right ventricular-vascular coupling during endotoxic shock in pigs. Artificial organs, 30(7), 560-4. https://doi.org/10.1111/j.1525-1594.2006.00260.x