Isoform 165 of vascular endothelial growth factor in collagen matrix improves ovine cryopreserved ovarian tissue revascularisation after xenotransplantation in mice

Laurie Henry, Soraya Labied, Maïté Fransolet, Nathalie Kirschvink, Silvia Blacher, Agnès Noel, Jean Michel Foidart, Michelle Nisolle, Carine Munaut

Research output: Contribution to journalArticle

Abstract

Background: Aggressive anti-cancer treatments can result in ovarian failure. Ovarian cryopreservation has been developed to preserve the fertility of young women, but early graft revascularisation still requires improvement. Methods: Frozen/thawed sheep ovarian cortical biopsies were embedded in collagen matrix with or without isoform 165 of vascular endothelial growth factor (VEGF165) and transplanted into ovaries of immunodeficient mice. Ovaries were chosen as transplantation sites to more closely resemble clinical conditions in which orthotopic transplantation has previously allowed several spontaneous pregnancies. Results: We found that VEGF165 significantly increased the number of Dextran-FITC positive functional vessels 3days after grafting. Dextran- fluorescein isothiocyanate (FITC) positive vessels were detectable in 53% and 29% of the mice in the VEGF-treated and control groups, respectively. Among these positive fragments, 50% in the treated group displayed mature smooth-muscle-actin-alpha (alpha-SMA) positive functional vessels compared with 0% in the control group. CD31 positive murine blood vessels were observed in 40% of the VEGF165 transplants compared with 21% of the controls. After 3weeks, the density of murine vessels was significantly higher in the VEGF165 group. Conclusion: The encapsulation of ovarian tissue in collagen matrix in the presence of VEGF165 before grafting has a positive effect on functional blood vessel recruitment. It can be considered as a useful technique to be improved and further developed before human clinical applications in female cancer patients in the context of fertility preservation.

Original languageEnglish
Article number12
JournalIn Reproductive Biology and endocrinology
Volume13
Issue number1
DOIs
Publication statusPublished - 7 Mar 2015

Fingerprint

Heterologous Transplantation
Blood Vessels
Ovary
Sheep
Protein Isoforms
Collagen
Transplantation
Fertility Preservation
Transplants
Control Groups
Cryopreservation
Vascular Endothelial Growth Factor A
Fertility
Smooth Muscle
Actins
Neoplasms
Biopsy
Pregnancy
fluorescein isothiocyanate dextran
human VEGFA protein

Keywords

  • Collagen matrix
  • Fertility preservation
  • VEGF165
  • Xenotransplantation and angiogenesis

Cite this

Henry, Laurie ; Labied, Soraya ; Fransolet, Maïté ; Kirschvink, Nathalie ; Blacher, Silvia ; Noel, Agnès ; Foidart, Jean Michel ; Nisolle, Michelle ; Munaut, Carine. / Isoform 165 of vascular endothelial growth factor in collagen matrix improves ovine cryopreserved ovarian tissue revascularisation after xenotransplantation in mice. In: In Reproductive Biology and endocrinology. 2015 ; Vol. 13, No. 1.
@article{2a56bd8f429340e0aa2891ba361e6f7d,
title = "Isoform 165 of vascular endothelial growth factor in collagen matrix improves ovine cryopreserved ovarian tissue revascularisation after xenotransplantation in mice",
abstract = "Background: Aggressive anti-cancer treatments can result in ovarian failure. Ovarian cryopreservation has been developed to preserve the fertility of young women, but early graft revascularisation still requires improvement. Methods: Frozen/thawed sheep ovarian cortical biopsies were embedded in collagen matrix with or without isoform 165 of vascular endothelial growth factor (VEGF165) and transplanted into ovaries of immunodeficient mice. Ovaries were chosen as transplantation sites to more closely resemble clinical conditions in which orthotopic transplantation has previously allowed several spontaneous pregnancies. Results: We found that VEGF165 significantly increased the number of Dextran-FITC positive functional vessels 3days after grafting. Dextran- fluorescein isothiocyanate (FITC) positive vessels were detectable in 53{\%} and 29{\%} of the mice in the VEGF-treated and control groups, respectively. Among these positive fragments, 50{\%} in the treated group displayed mature smooth-muscle-actin-alpha (alpha-SMA) positive functional vessels compared with 0{\%} in the control group. CD31 positive murine blood vessels were observed in 40{\%} of the VEGF165 transplants compared with 21{\%} of the controls. After 3weeks, the density of murine vessels was significantly higher in the VEGF165 group. Conclusion: The encapsulation of ovarian tissue in collagen matrix in the presence of VEGF165 before grafting has a positive effect on functional blood vessel recruitment. It can be considered as a useful technique to be improved and further developed before human clinical applications in female cancer patients in the context of fertility preservation.",
keywords = "Collagen matrix, Fertility preservation, VEGF165, Xenotransplantation and angiogenesis",
author = "Laurie Henry and Soraya Labied and Ma{\"i}t{\'e} Fransolet and Nathalie Kirschvink and Silvia Blacher and Agn{\`e}s Noel and Foidart, {Jean Michel} and Michelle Nisolle and Carine Munaut",
year = "2015",
month = "3",
day = "7",
doi = "10.1186/s12958-015-0015-2",
language = "English",
volume = "13",
journal = "In Reproductive Biology and endocrinology",
number = "1",

}

Isoform 165 of vascular endothelial growth factor in collagen matrix improves ovine cryopreserved ovarian tissue revascularisation after xenotransplantation in mice. / Henry, Laurie; Labied, Soraya; Fransolet, Maïté; Kirschvink, Nathalie; Blacher, Silvia; Noel, Agnès; Foidart, Jean Michel; Nisolle, Michelle; Munaut, Carine.

In: In Reproductive Biology and endocrinology, Vol. 13, No. 1, 12, 07.03.2015.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Isoform 165 of vascular endothelial growth factor in collagen matrix improves ovine cryopreserved ovarian tissue revascularisation after xenotransplantation in mice

AU - Henry, Laurie

AU - Labied, Soraya

AU - Fransolet, Maïté

AU - Kirschvink, Nathalie

AU - Blacher, Silvia

AU - Noel, Agnès

AU - Foidart, Jean Michel

AU - Nisolle, Michelle

AU - Munaut, Carine

PY - 2015/3/7

Y1 - 2015/3/7

N2 - Background: Aggressive anti-cancer treatments can result in ovarian failure. Ovarian cryopreservation has been developed to preserve the fertility of young women, but early graft revascularisation still requires improvement. Methods: Frozen/thawed sheep ovarian cortical biopsies were embedded in collagen matrix with or without isoform 165 of vascular endothelial growth factor (VEGF165) and transplanted into ovaries of immunodeficient mice. Ovaries were chosen as transplantation sites to more closely resemble clinical conditions in which orthotopic transplantation has previously allowed several spontaneous pregnancies. Results: We found that VEGF165 significantly increased the number of Dextran-FITC positive functional vessels 3days after grafting. Dextran- fluorescein isothiocyanate (FITC) positive vessels were detectable in 53% and 29% of the mice in the VEGF-treated and control groups, respectively. Among these positive fragments, 50% in the treated group displayed mature smooth-muscle-actin-alpha (alpha-SMA) positive functional vessels compared with 0% in the control group. CD31 positive murine blood vessels were observed in 40% of the VEGF165 transplants compared with 21% of the controls. After 3weeks, the density of murine vessels was significantly higher in the VEGF165 group. Conclusion: The encapsulation of ovarian tissue in collagen matrix in the presence of VEGF165 before grafting has a positive effect on functional blood vessel recruitment. It can be considered as a useful technique to be improved and further developed before human clinical applications in female cancer patients in the context of fertility preservation.

AB - Background: Aggressive anti-cancer treatments can result in ovarian failure. Ovarian cryopreservation has been developed to preserve the fertility of young women, but early graft revascularisation still requires improvement. Methods: Frozen/thawed sheep ovarian cortical biopsies were embedded in collagen matrix with or without isoform 165 of vascular endothelial growth factor (VEGF165) and transplanted into ovaries of immunodeficient mice. Ovaries were chosen as transplantation sites to more closely resemble clinical conditions in which orthotopic transplantation has previously allowed several spontaneous pregnancies. Results: We found that VEGF165 significantly increased the number of Dextran-FITC positive functional vessels 3days after grafting. Dextran- fluorescein isothiocyanate (FITC) positive vessels were detectable in 53% and 29% of the mice in the VEGF-treated and control groups, respectively. Among these positive fragments, 50% in the treated group displayed mature smooth-muscle-actin-alpha (alpha-SMA) positive functional vessels compared with 0% in the control group. CD31 positive murine blood vessels were observed in 40% of the VEGF165 transplants compared with 21% of the controls. After 3weeks, the density of murine vessels was significantly higher in the VEGF165 group. Conclusion: The encapsulation of ovarian tissue in collagen matrix in the presence of VEGF165 before grafting has a positive effect on functional blood vessel recruitment. It can be considered as a useful technique to be improved and further developed before human clinical applications in female cancer patients in the context of fertility preservation.

KW - Collagen matrix

KW - Fertility preservation

KW - VEGF165

KW - Xenotransplantation and angiogenesis

UR - http://www.scopus.com/inward/record.url?scp=84925244178&partnerID=8YFLogxK

U2 - 10.1186/s12958-015-0015-2

DO - 10.1186/s12958-015-0015-2

M3 - Article

VL - 13

JO - In Reproductive Biology and endocrinology

JF - In Reproductive Biology and endocrinology

IS - 1

M1 - 12

ER -