Iron nanoparticles decorated multi-wall carbon nanotubes modified carbon paste electrode as an electrochemical sensor for the simultaneous determination of uric acid in the presence of ascorbic acid, dopamine and l-tyrosine

Arvind Kumar Bhakta, Ronald J. Mascarenhas, Ozma J. D'Souza, Ashis K. Satpati, Simon Detriche, Zineb Mekhalif, Joseph Delhalle

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Abstract

Iron nanoparticles decorated multi-wall carbon nanotubes modified carbon paste electrode (Fe-MWCNTs/MCPE) was prepared by bulk-modification method. The electrochemical impedance spectroscopy (EIS) suggests least charge transfer resistance at the modified electrode. The electrochemical behavior of UA was studied in 0.1 M phosphate buffer solution (PBS) of pH 3.0 using cyclic voltammetry (CV) while differential pulse voltammetry (DPV) was used for quantification. The spectroelectrochemial study of oxidation of UA at Fe-MWCNTs/MCPE showed a decrease in the absorbance of two peaks with time, which are ascribed to π to π∗ and n to π∗ transitions. Under optimum condition, the DPV response offered two linear dynamic ranges for UA in the concentration range 7.0 × 10<sup>- 8</sup> M-1.0 × 10<sup>- 6</sup> M and 2.0 × 10<sup>- 6</sup> M-1.0 × 10<sup>- 5</sup> M with detection limit (4.80 ± 0.35) × 10<sup>- 8</sup> M (S/N = 3). The practical analytical application of this sensor was successfully evaluated by determination of spiked UA in clinical samples, such as human blood serum and urine with good percentage recovery. The proposed electrochemical sensor offers a simple, reliable, rapid, reproducible and cost effective analysis of a quaternary mixture of biomolecules containing AA, DA, UA and Tyr which was free from mutual interferences.

Original languageEnglish
Pages (from-to)328-337
Number of pages10
JournalMaterials science and engineering C
Volume57
DOIs
Publication statusPublished - 17 Aug 2015

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uric acid
dopamine
Electrochemical sensors
Carbon Nanotubes
ascorbic acid
tyrosine
Ascorbic acid
Voltammetry
Ointments
Uric Acid
Ascorbic Acid
Tyrosine
Dopamine
Carbon nanotubes
Carbon
Iron
carbon nanotubes
blood serum
Nanoparticles
iron

Keywords

  • Iron nanoparticles decorated multi-wall carbon nanotubes
  • Uric acid
  • Ascorbic acid
  • Dopamine
  • L-Tyrosine
  • Differential pulse voltammetry

Cite this

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title = "Iron nanoparticles decorated multi-wall carbon nanotubes modified carbon paste electrode as an electrochemical sensor for the simultaneous determination of uric acid in the presence of ascorbic acid, dopamine and l-tyrosine",
abstract = "Iron nanoparticles decorated multi-wall carbon nanotubes modified carbon paste electrode (Fe-MWCNTs/MCPE) was prepared by bulk-modification method. The electrochemical impedance spectroscopy (EIS) suggests least charge transfer resistance at the modified electrode. The electrochemical behavior of UA was studied in 0.1 M phosphate buffer solution (PBS) of pH 3.0 using cyclic voltammetry (CV) while differential pulse voltammetry (DPV) was used for quantification. The spectroelectrochemial study of oxidation of UA at Fe-MWCNTs/MCPE showed a decrease in the absorbance of two peaks with time, which are ascribed to π to π∗ and n to π∗ transitions. Under optimum condition, the DPV response offered two linear dynamic ranges for UA in the concentration range 7.0 × 10- 8 M-1.0 × 10- 6 M and 2.0 × 10- 6 M-1.0 × 10- 5 M with detection limit (4.80 ± 0.35) × 10- 8 M (S/N = 3). The practical analytical application of this sensor was successfully evaluated by determination of spiked UA in clinical samples, such as human blood serum and urine with good percentage recovery. The proposed electrochemical sensor offers a simple, reliable, rapid, reproducible and cost effective analysis of a quaternary mixture of biomolecules containing AA, DA, UA and Tyr which was free from mutual interferences.",
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author = "Bhakta, {Arvind Kumar} and Mascarenhas, {Ronald J.} and D'Souza, {Ozma J.} and Satpati, {Ashis K.} and Simon Detriche and Zineb Mekhalif and Joseph Delhalle",
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T1 - Iron nanoparticles decorated multi-wall carbon nanotubes modified carbon paste electrode as an electrochemical sensor for the simultaneous determination of uric acid in the presence of ascorbic acid, dopamine and l-tyrosine

AU - Bhakta, Arvind Kumar

AU - Mascarenhas, Ronald J.

AU - D'Souza, Ozma J.

AU - Satpati, Ashis K.

AU - Detriche, Simon

AU - Mekhalif, Zineb

AU - Delhalle, Joseph

PY - 2015/8/17

Y1 - 2015/8/17

N2 - Iron nanoparticles decorated multi-wall carbon nanotubes modified carbon paste electrode (Fe-MWCNTs/MCPE) was prepared by bulk-modification method. The electrochemical impedance spectroscopy (EIS) suggests least charge transfer resistance at the modified electrode. The electrochemical behavior of UA was studied in 0.1 M phosphate buffer solution (PBS) of pH 3.0 using cyclic voltammetry (CV) while differential pulse voltammetry (DPV) was used for quantification. The spectroelectrochemial study of oxidation of UA at Fe-MWCNTs/MCPE showed a decrease in the absorbance of two peaks with time, which are ascribed to π to π∗ and n to π∗ transitions. Under optimum condition, the DPV response offered two linear dynamic ranges for UA in the concentration range 7.0 × 10- 8 M-1.0 × 10- 6 M and 2.0 × 10- 6 M-1.0 × 10- 5 M with detection limit (4.80 ± 0.35) × 10- 8 M (S/N = 3). The practical analytical application of this sensor was successfully evaluated by determination of spiked UA in clinical samples, such as human blood serum and urine with good percentage recovery. The proposed electrochemical sensor offers a simple, reliable, rapid, reproducible and cost effective analysis of a quaternary mixture of biomolecules containing AA, DA, UA and Tyr which was free from mutual interferences.

AB - Iron nanoparticles decorated multi-wall carbon nanotubes modified carbon paste electrode (Fe-MWCNTs/MCPE) was prepared by bulk-modification method. The electrochemical impedance spectroscopy (EIS) suggests least charge transfer resistance at the modified electrode. The electrochemical behavior of UA was studied in 0.1 M phosphate buffer solution (PBS) of pH 3.0 using cyclic voltammetry (CV) while differential pulse voltammetry (DPV) was used for quantification. The spectroelectrochemial study of oxidation of UA at Fe-MWCNTs/MCPE showed a decrease in the absorbance of two peaks with time, which are ascribed to π to π∗ and n to π∗ transitions. Under optimum condition, the DPV response offered two linear dynamic ranges for UA in the concentration range 7.0 × 10- 8 M-1.0 × 10- 6 M and 2.0 × 10- 6 M-1.0 × 10- 5 M with detection limit (4.80 ± 0.35) × 10- 8 M (S/N = 3). The practical analytical application of this sensor was successfully evaluated by determination of spiked UA in clinical samples, such as human blood serum and urine with good percentage recovery. The proposed electrochemical sensor offers a simple, reliable, rapid, reproducible and cost effective analysis of a quaternary mixture of biomolecules containing AA, DA, UA and Tyr which was free from mutual interferences.

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