TY - JOUR
T1 - Integrated multi-biomarker responses of juvenile rainbow trout (Oncorhynchus mykiss) to an environmentally relevant pharmaceutical mixture
AU - Beghin, Mahaut
AU - Schmitz, Mélodie
AU - Betoulle, Stéphane
AU - Palluel, Olivier
AU - Baekelandt, Sébastien
AU - Mandiki, Syaghalirwa N.M.
AU - Gillet, Erin
AU - Nott, Katherine
AU - Porcher, Jean Marc
AU - Robert, Christelle
AU - Ronkart, Sébastien
AU - Kestemont, Patrick
N1 - Funding Information:
This work was carried out in the framework of the Project DIADEM (Développement d'une Approche Intégrée pour le Diagnostic de la qualité des Eaux de la Meuse) and was supported by Interreg France-Wallonia-Vlaanderen Programme (European Regional Development Development Fund ERDF) and the Public Service of Wallonia (SPW DGO3) (Belgium). We are grateful to the scientific and technical staff of the Research Unit in Environmental and Evolutionary Biology (URBE) of the University of Namur for their support during fish organ sampling. The authors thank all the members of the DIADeM project who provided help for data collection and analysis.
Publisher Copyright:
© 2021 The Authors
PY - 2021/9/15
Y1 - 2021/9/15
N2 - Pharmaceuticals are emerging pollutants of concern for aquatic ecosystems where they are occurring in complex mixtures. In the present study, the chronic toxicity of an environmentally relevant pharmaceutical mixture on juvenile rainbow trout (Oncorhynchus mykiss) was investigated. Five pharmaceuticals (paracetamol, carbamazepine, diclofenac, naproxen and irbesartan) were selected based on their detection frequency and concentration levels in the Meuse river (Belgium). Fish were exposed for 42 days to three different concentrations of the mixture, the median one detected in the Meuse river, 10-times and 100-times this concentration. Effects on the nervous, immune, antioxidant, and detoxification systems were evaluated throughout the exposure period and their response standardized using the Integrated Biomarker Response (IBRv2) index. IBRv2 scores increased over time in the fish exposed to the highest concentration. After 42 days, fish exposed to the highest concentration displayed significantly higher levels in lysozyme activity (p < 0.01). The mixture also caused significant changes in brain serotonin turnover (p < 0.05). In short, our results indicate that the subchronic waterborne exposure to a pharmaceutical mixture commonly occurring in freshwater ecosystems may affect the neuroendocrine and immune systems of juvenile rainbow trout.
AB - Pharmaceuticals are emerging pollutants of concern for aquatic ecosystems where they are occurring in complex mixtures. In the present study, the chronic toxicity of an environmentally relevant pharmaceutical mixture on juvenile rainbow trout (Oncorhynchus mykiss) was investigated. Five pharmaceuticals (paracetamol, carbamazepine, diclofenac, naproxen and irbesartan) were selected based on their detection frequency and concentration levels in the Meuse river (Belgium). Fish were exposed for 42 days to three different concentrations of the mixture, the median one detected in the Meuse river, 10-times and 100-times this concentration. Effects on the nervous, immune, antioxidant, and detoxification systems were evaluated throughout the exposure period and their response standardized using the Integrated Biomarker Response (IBRv2) index. IBRv2 scores increased over time in the fish exposed to the highest concentration. After 42 days, fish exposed to the highest concentration displayed significantly higher levels in lysozyme activity (p < 0.01). The mixture also caused significant changes in brain serotonin turnover (p < 0.05). In short, our results indicate that the subchronic waterborne exposure to a pharmaceutical mixture commonly occurring in freshwater ecosystems may affect the neuroendocrine and immune systems of juvenile rainbow trout.
KW - Cocktail
KW - Drugs
KW - Fish
KW - IBR
KW - Immunity
KW - Neurotoxicity
UR - http://www.scopus.com/inward/record.url?scp=85108881302&partnerID=8YFLogxK
U2 - 10.1016/j.ecoenv.2021.112454
DO - 10.1016/j.ecoenv.2021.112454
M3 - Article
AN - SCOPUS:85108881302
SN - 0147-6513
VL - 221
JO - Ecotoxicology and Environmental Safety
JF - Ecotoxicology and Environmental Safety
M1 - 112454
ER -