Abstract
Objective. To investigate the regulation of the prostaglandin (PG) synthesis by interleukin 1 (IL-1) in human synovial cells and chondrocytes. Methods. Both cell types stimulated by human recombinant IL-1 synthesized PGE2, PGF(2α) and 6-keto-PGF(1α). Results. PGE2 was the major PG synthesized. When arachidonic acid was added exogenously at the end of the stimulation, an increase in the prostaglandin synthesis was observed after 6 and 24 h suggesting that cyclooxygenase is the limiting enzyme. Using actinomycin D and cycloheximide. PG synthesis was shown to be protein synthesis dependent. Inhibition of the constitutive cyclooxygenase by aspirin before the IL-1 stimulation confirmed that the increased prostaglandin synthesis was due to a de novo synthesis of cyclooxygenase. Conclusion. This enzyme induction by IL-1 was found to be similar in both cell types.
Original language | English |
---|---|
Pages (from-to) | 462-466 |
Number of pages | 5 |
Journal | Journal of Rheumatology |
Volume | 21 |
Issue number | 3 |
Publication status | Published - 1994 |
Keywords
- CHONDROCYTES
- CYCLOOXYGENASE
- INTERLEUKIN 1
- SYNOVIAL CELLS