In vivo response of some immune and endocrine variables to LPS in Eurasian perch (Perca fluviatilis, L.) and modulation of this response by two corticosteroids, cortisol and 11-deoxycorticosterone

Cédric Mathieu, Sylvain Milla, Robert Mandiki, Jessica Douxfils, Patrick Kestemont

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Abstract

In fish, the endocrine system, especially corticosteroids pathway, strongly interacts with immune system. On the other hand, in vivo co-stimulation of both systems is not well documented. To better understand this interaction, we decided to evaluate the in vivo effects of both stimulation of the immune system and co-stimulation of both systems in Eurasian perch juveniles. Fish were injected either with 10mgkg-1 LPS, or with a combination of LPS and 0.8mgkg-1 cortisol or LPS and 0.08mgkg-1 11-deoxycorticosterone (DOC) and sampled 1, 3 or 7days after injection. LPS affected the immune system by increasing plasma lysozyme activity and blood neutrophils populations. During the same time-course, LPS decreased the proportion of a mixture of lymphocytes and thrombocytes in blood and TNF-α expression in spleen. Cortisol modulated the LPS-mediated response in TNF-α mRNA expression levels in spleen. Contrary to LPS alone, the association of LPS with DOC modulated the abundance of complement component 3 (C3) mRNA in spleen. On the other hand, LPS altered the corticotropic axis by decreasing mRNA expression levels of all corticosteroid receptors and of 11β-HSD-2 in spleen. Both corticosteroids injected were not able to balance these LPS-induced suppressive effects on corticosteroid receptors and 11β-HSD-2 expression levels in spleen. Contrary to LPS alone, the association of LPS with DOC modulated GR-1b expression in gills. These results indicated that LPS is a strong modulator of the corticosteroid receptors expression in spleen. Furthermore, we report for the first time a LPS-induced decrease of the mineralocorticoid receptor expression. Finally, corticosteroids were able to modulate the LPS-mediated response at the transcriptional level. © 2013 Elsevier Inc.

Original languageEnglish
Pages (from-to)25-34
Number of pages10
JournalComparative biochemistry and physiology. Part A, Molecular & integrative physiology
Volume167
DOIs
Publication statusPublished - 14 Sep 2013

Keywords

  • 11-Deoxycorticosterone (DOC)
  • Corticosteroid receptors
  • Cortisol
  • Fish
  • Immunity
  • Intraperitoneal injection
  • Lipopolysaccharide (LPS)
  • Perca fluviatilis

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