In vitro models of dermatophyte infection to investigate epidermal barrier alterations

Research output: Contribution to journalReview article

3 Downloads (Pure)

Abstract

Fungal infections of the skin, known as dermatophytoses, are initiated at the epidermal barrier and lead to dysfunctions of the stratum corneum and cornified skin appendages. Dermatophytosis affects a significant part of the human population and, despite the availability of effective treatments, its prevalence is still increasing. Numerous dermatophyte species are able to induce lesions in both animals and humans, with different clinical pictures and host inflammatory responses. The understanding of the infectious process and of tissue responses has been impeded by discrepancies between observations in vivo or in research models. Indeed, cells cultured as monolayers do not undergo the keratinization process required to study the adherence and invasion of dermatophytes. Animal models lack relevance to study human dermatophytosis because of species-specific differences in the development of lesions and inflammatory responses. This review focuses on the recent development of cultured human skin equivalents, which partly overcomes those limitations and allows improved understanding of the pathogenesis of dermatophytosis in human being, especially the impacts of infection on epidermal barrier integrity.

Original languageEnglish
Pages (from-to)915-922
Number of pages8
JournalExperimental dermatology
Volume27
Issue number8
Early online date29 Jun 2018
DOIs
Publication statusPublished - 28 Aug 2018

Fingerprint

Arthrodermataceae
Tinea
Skin
Animals
Infection
Mycoses
Monolayers
Human Development
Availability
Cornea
Tissue
Cultured Cells
Animal Models
In Vitro Techniques
Research
Population

Keywords

  • epidermal barrier
  • fungal infection
  • model of dermatophytosis
  • reconstructed human epidermis
  • trichophyton rubrum

Cite this

@article{c7c004ee20754d09b99d3cc52988cf48,
title = "In vitro models of dermatophyte infection to investigate epidermal barrier alterations",
abstract = "Fungal infections of the skin, known as dermatophytoses, are initiated at the epidermal barrier and lead to dysfunctions of the stratum corneum and cornified skin appendages. Dermatophytosis affects a significant part of the human population and, despite the availability of effective treatments, its prevalence is still increasing. Numerous dermatophyte species are able to induce lesions in both animals and humans, with different clinical pictures and host inflammatory responses. The understanding of the infectious process and of tissue responses has been impeded by discrepancies between observations in vivo or in research models. Indeed, cells cultured as monolayers do not undergo the keratinization process required to study the adherence and invasion of dermatophytes. Animal models lack relevance to study human dermatophytosis because of species-specific differences in the development of lesions and inflammatory responses. This review focuses on the recent development of cultured human skin equivalents, which partly overcomes those limitations and allows improved understanding of the pathogenesis of dermatophytosis in human being, especially the impacts of infection on epidermal barrier integrity.",
keywords = "epidermal barrier, fungal infection, model of dermatophytosis, reconstructed human epidermis, trichophyton rubrum",
author = "{\'E}milie Faway and {Lambert De Rouvroit}, Catherine and Yves Poumay",
year = "2018",
month = "8",
day = "28",
doi = "doi: 10.1111/exd.13726",
language = "English",
volume = "27",
pages = "915--922",
journal = "Experimental dermatology",
issn = "0906-6705",
publisher = "Wiley-Blackwell",
number = "8",

}

TY - JOUR

T1 - In vitro models of dermatophyte infection to investigate epidermal barrier alterations

AU - Faway, Émilie

AU - Lambert De Rouvroit, Catherine

AU - Poumay, Yves

PY - 2018/8/28

Y1 - 2018/8/28

N2 - Fungal infections of the skin, known as dermatophytoses, are initiated at the epidermal barrier and lead to dysfunctions of the stratum corneum and cornified skin appendages. Dermatophytosis affects a significant part of the human population and, despite the availability of effective treatments, its prevalence is still increasing. Numerous dermatophyte species are able to induce lesions in both animals and humans, with different clinical pictures and host inflammatory responses. The understanding of the infectious process and of tissue responses has been impeded by discrepancies between observations in vivo or in research models. Indeed, cells cultured as monolayers do not undergo the keratinization process required to study the adherence and invasion of dermatophytes. Animal models lack relevance to study human dermatophytosis because of species-specific differences in the development of lesions and inflammatory responses. This review focuses on the recent development of cultured human skin equivalents, which partly overcomes those limitations and allows improved understanding of the pathogenesis of dermatophytosis in human being, especially the impacts of infection on epidermal barrier integrity.

AB - Fungal infections of the skin, known as dermatophytoses, are initiated at the epidermal barrier and lead to dysfunctions of the stratum corneum and cornified skin appendages. Dermatophytosis affects a significant part of the human population and, despite the availability of effective treatments, its prevalence is still increasing. Numerous dermatophyte species are able to induce lesions in both animals and humans, with different clinical pictures and host inflammatory responses. The understanding of the infectious process and of tissue responses has been impeded by discrepancies between observations in vivo or in research models. Indeed, cells cultured as monolayers do not undergo the keratinization process required to study the adherence and invasion of dermatophytes. Animal models lack relevance to study human dermatophytosis because of species-specific differences in the development of lesions and inflammatory responses. This review focuses on the recent development of cultured human skin equivalents, which partly overcomes those limitations and allows improved understanding of the pathogenesis of dermatophytosis in human being, especially the impacts of infection on epidermal barrier integrity.

KW - epidermal barrier

KW - fungal infection

KW - model of dermatophytosis

KW - reconstructed human epidermis

KW - trichophyton rubrum

UR - http://www.scopus.com/inward/record.url?scp=85052514843&partnerID=8YFLogxK

U2 - doi: 10.1111/exd.13726

DO - doi: 10.1111/exd.13726

M3 - Review article

VL - 27

SP - 915

EP - 922

JO - Experimental dermatology

JF - Experimental dermatology

SN - 0906-6705

IS - 8

ER -