TY - JOUR
T1 - In vitro effects of oxygen on physico-chemical properties of horse erythrocyte membrane
AU - PORTIER, Karine
AU - GUICHARDANT, Michel
AU - DEBOUZY, Jean-Claude
AU - CROUZIER, David
AU - GERAUD, Ingrid
AU - Kirschvink, Nathalie
AU - LEKEUX, Pierre
AU - FELLMANN, Nicole
AU - COUDERT, Jean
PY - 2007
Y1 - 2007
N2 - Whether direct exposure to different concentrations (0%, 13%, 100%) of oxygen may affect horse erythrocyte membrane fluidity (EMF) and fatty acid (FA) composition was studied during 1 (T60) and 2 h (T120) exposure. EMF was investigated at the head group level and hydrophobic core thanks to phosphorus nucleus 31 (31P) nuclear magnetic resonance (31P NMR) and electronic paramagnetic resonance (EPR) using two spin probes: 5-nitroxydestearic acid and 16-doxylstearic acid. Lipid structure of the membranes was studied by gas liquid chromatography. 4-Hydroxy-2E-nonenal was also analyzed as a marker of lipid peroxidation. It increased at T120 13% and 100% oxygen whereas there were no significant changes in membrane dynamic or structure. Correlation was demonstrated between EMF and partial pressure of oxygen in the blood (PO2). In vitro high rate of oxygenation was efficient to induce lipid peroxidation but did not change membrane dynamics. This may be due to a low free radical production in vitro or to the high red blood cells antioxidant properties.
AB - Whether direct exposure to different concentrations (0%, 13%, 100%) of oxygen may affect horse erythrocyte membrane fluidity (EMF) and fatty acid (FA) composition was studied during 1 (T60) and 2 h (T120) exposure. EMF was investigated at the head group level and hydrophobic core thanks to phosphorus nucleus 31 (31P) nuclear magnetic resonance (31P NMR) and electronic paramagnetic resonance (EPR) using two spin probes: 5-nitroxydestearic acid and 16-doxylstearic acid. Lipid structure of the membranes was studied by gas liquid chromatography. 4-Hydroxy-2E-nonenal was also analyzed as a marker of lipid peroxidation. It increased at T120 13% and 100% oxygen whereas there were no significant changes in membrane dynamic or structure. Correlation was demonstrated between EMF and partial pressure of oxygen in the blood (PO2). In vitro high rate of oxygenation was efficient to induce lipid peroxidation but did not change membrane dynamics. This may be due to a low free radical production in vitro or to the high red blood cells antioxidant properties.
KW - Erythrocyte; Oxygen; Lipid peroxidation; Membrane fluidity; 4 HNE
M3 - Article
VL - 23
SP - 340
EP - 346
JO - Environmental Toxicology and Pharmacology
JF - Environmental Toxicology and Pharmacology
ER -