Impaired blood-brain and blood-spinal cord barriers in mutant SOD1-linked ALS rat

Charles Nicaise, Dinko Mitrecic, Pieter Demetter, Robert De Decker, Michèle Authelet, Alain Boom, Roland Pochet

Research output: Contribution to journalArticle

Abstract

Blood-brain barrier (BBB) and blood-spinal cord barrier (BSCB) impairment is an additional accident occurring during the amyotrophic lateral sclerosis (ALS) progression. In this work, we aimed to decipher if BBB/BSCB leakage appeared before critical detrimental events and could serve as a marker preceding clinical symptoms. Three different BBB leakage markers: Evans blue, IgG and hemosiderin, were used to look at the SOD1-linked ALS rat model at presymptomatic and symptomatic stages. Although IgG and hemosiderin could be detected at presymptomatic stage, Evans blue extravasation which fits best with BBB/BSCB impairment could only be seen at symptomatic stages. BBB/BSCB impairment was further substantiate by showing at symptomatic stages decreased mRNA expression of ZO-1 and occludin as well as agrin, a basal membrane constituent. Electron microscopic data substantiate a toxic environment around endothelial cell and peri-vascular swollen astrocyte end-feet showing oedema-linked BBB opening.
Original languageEnglish
Pages (from-to)152-62
Number of pages11
JournalBrain research
Volume1301
DOIs
Publication statusPublished - 8 Dec 2009

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Amyotrophic Lateral Sclerosis
Blood-Brain Barrier
Spinal Cord
Brain
Hemosiderin
Evans Blue
Immunoglobulin G
Agrin
Occludin
Poisons
Astrocytes
Accidents
Edema
Endothelial Cells
Biomarkers
Electrons
Messenger RNA
Membranes

Keywords

  • Agrin
  • Amyotrophic Lateral Sclerosis
  • Animals
  • Astrocytes
  • Biological Markers
  • Blood-Brain Barrier
  • Brain Stem
  • Cell Shape
  • Disease Models, Animal
  • Endothelial Cells
  • Enzyme-Linked Immunosorbent Assay
  • Evans Blue
  • Hemosiderin
  • Immunoglobulins
  • Immunohistochemistry
  • Membrane Proteins
  • Microscopy, Electron
  • Occludin
  • Phosphoproteins
  • RNA, Messenger
  • Rats
  • Rats, Transgenic
  • Reverse Transcriptase Polymerase Chain Reaction
  • Spinal Cord
  • Staining and Labeling
  • Superoxide Dismutase
  • Zonula Occludens-1 Protein

Cite this

Nicaise, C., Mitrecic, D., Demetter, P., De Decker, R., Authelet, M., Boom, A., & Pochet, R. (2009). Impaired blood-brain and blood-spinal cord barriers in mutant SOD1-linked ALS rat. Brain research, 1301, 152-62. https://doi.org/10.1016/j.brainres.2009.09.018
Nicaise, Charles ; Mitrecic, Dinko ; Demetter, Pieter ; De Decker, Robert ; Authelet, Michèle ; Boom, Alain ; Pochet, Roland. / Impaired blood-brain and blood-spinal cord barriers in mutant SOD1-linked ALS rat. In: Brain research. 2009 ; Vol. 1301. pp. 152-62.
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Nicaise, C, Mitrecic, D, Demetter, P, De Decker, R, Authelet, M, Boom, A & Pochet, R 2009, 'Impaired blood-brain and blood-spinal cord barriers in mutant SOD1-linked ALS rat', Brain research, vol. 1301, pp. 152-62. https://doi.org/10.1016/j.brainres.2009.09.018

Impaired blood-brain and blood-spinal cord barriers in mutant SOD1-linked ALS rat. / Nicaise, Charles; Mitrecic, Dinko; Demetter, Pieter; De Decker, Robert; Authelet, Michèle; Boom, Alain; Pochet, Roland.

In: Brain research, Vol. 1301, 08.12.2009, p. 152-62.

Research output: Contribution to journalArticle

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T1 - Impaired blood-brain and blood-spinal cord barriers in mutant SOD1-linked ALS rat

AU - Nicaise, Charles

AU - Mitrecic, Dinko

AU - Demetter, Pieter

AU - De Decker, Robert

AU - Authelet, Michèle

AU - Boom, Alain

AU - Pochet, Roland

PY - 2009/12/8

Y1 - 2009/12/8

N2 - Blood-brain barrier (BBB) and blood-spinal cord barrier (BSCB) impairment is an additional accident occurring during the amyotrophic lateral sclerosis (ALS) progression. In this work, we aimed to decipher if BBB/BSCB leakage appeared before critical detrimental events and could serve as a marker preceding clinical symptoms. Three different BBB leakage markers: Evans blue, IgG and hemosiderin, were used to look at the SOD1-linked ALS rat model at presymptomatic and symptomatic stages. Although IgG and hemosiderin could be detected at presymptomatic stage, Evans blue extravasation which fits best with BBB/BSCB impairment could only be seen at symptomatic stages. BBB/BSCB impairment was further substantiate by showing at symptomatic stages decreased mRNA expression of ZO-1 and occludin as well as agrin, a basal membrane constituent. Electron microscopic data substantiate a toxic environment around endothelial cell and peri-vascular swollen astrocyte end-feet showing oedema-linked BBB opening.

AB - Blood-brain barrier (BBB) and blood-spinal cord barrier (BSCB) impairment is an additional accident occurring during the amyotrophic lateral sclerosis (ALS) progression. In this work, we aimed to decipher if BBB/BSCB leakage appeared before critical detrimental events and could serve as a marker preceding clinical symptoms. Three different BBB leakage markers: Evans blue, IgG and hemosiderin, were used to look at the SOD1-linked ALS rat model at presymptomatic and symptomatic stages. Although IgG and hemosiderin could be detected at presymptomatic stage, Evans blue extravasation which fits best with BBB/BSCB impairment could only be seen at symptomatic stages. BBB/BSCB impairment was further substantiate by showing at symptomatic stages decreased mRNA expression of ZO-1 and occludin as well as agrin, a basal membrane constituent. Electron microscopic data substantiate a toxic environment around endothelial cell and peri-vascular swollen astrocyte end-feet showing oedema-linked BBB opening.

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KW - Amyotrophic Lateral Sclerosis

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KW - Astrocytes

KW - Biological Markers

KW - Blood-Brain Barrier

KW - Brain Stem

KW - Cell Shape

KW - Disease Models, Animal

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KW - Enzyme-Linked Immunosorbent Assay

KW - Evans Blue

KW - Hemosiderin

KW - Immunoglobulins

KW - Immunohistochemistry

KW - Membrane Proteins

KW - Microscopy, Electron

KW - Occludin

KW - Phosphoproteins

KW - RNA, Messenger

KW - Rats

KW - Rats, Transgenic

KW - Reverse Transcriptase Polymerase Chain Reaction

KW - Spinal Cord

KW - Staining and Labeling

KW - Superoxide Dismutase

KW - Zonula Occludens-1 Protein

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DO - 10.1016/j.brainres.2009.09.018

M3 - Article

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VL - 1301

SP - 152

EP - 162

JO - Brain research

JF - Brain research

SN - 1872-6240

ER -