Abstract
Mycobacterium avium subsp. paratuberculosis (Map), the etiological agent of chronic enteritis of the small intestine in domestic and wild ruminants, causes substantial losses to livestock industry. Control of this disease is seriously hampered by the lack of adequate diagnostic tools and vaccines. Here we report on the immunogenicity of eight Map specific antigens, i.e. MAP1693c, Ag3, MAP2677c (identified by post-genomic and immunoproteomic analysis of Map secretome) and Ag5, Ag6, MAP1637c, MAP0388 and MAP3743 (identified by bioinformatic in silico screening of the Map genome). Strong, antigen-specific IFN-γ responses were induced in mice vaccinated with plasmid DNA encoding MAP1693c, MAP1637c, MAP0388 and MAP3743. In contrast, T cell responses in Map infected mice were directed preferentially against Ag5 and to a lesser extent against MAP3743. None of the tested DNA vaccines conferred protection against subsequent challenge with Map.
Original language | English |
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Pages (from-to) | 74-85 |
Number of pages | 12 |
Journal | Veterinary immunology and immunopathology |
Volume | 145 |
Issue number | 1-2 |
DOIs | |
Publication status | Published - 2012 |
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Dive into the research topics of 'Immunogenicity of eight Mycobacterium avium subsp. paratuberculosis specific antigens in DNA vaccinated and Map infected mice'. Together they form a unique fingerprint.Student theses
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Développement d'outils de diagnostic pour le contrôle de la paratuberculose bovine : détection d'anticorps polyclonaux dans des échantillons de sérum et de colostrum à l'aide d'un ELISA et production d'anticorps monoclonaux
Author: Roupie, V., 2002Supervisor: Letesson, J. (Supervisor)
Student thesis: Master types › Master in Biochemistry and molecular and cellular Biology
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Identification et caractérisation immunologique d'antigènes spécifiques de Mycobacterium avium subsp. Paratuberculosis à des fins vaccinales
Author: Roupie, V., 16 Mar 2012Supervisor: Letesson, J. (Supervisor), Muylkens, B. (President), Huygen, K. (External person) (Jury), Wattiez, R. (External person) (Jury) & HERMAN, L. (External person) (Jury)
Student thesis: Doc types › Doctor of Sciences