Identification of the major T-cell antigens present in the Brucella melitensis B115 protein preparation, brucellergene OCB

Philippe Denoel; T.K.O. Vo; Vincent Weynants; Anne Tibor; Dominique Gilson; Michel Zygmunt; Joseph Limet; Jean-Jacques Letesson

doi:10.1099/00222615-46-9-801

Identification of the major T-cell antigens present in the Brucella melitensis B115 protein preparation, brucellergene OCB

Philippe Denoel, T.K.O. Vo, Vincent Weynants, Anne Tibor, Dominique Gilson, Michel Zygmunt, Joseph Limet, Jean-Jacques Letesson

Research output: Contribution to journal › Article

Abstract

Brucellergene is a commercial allergen prepared from Brucella melitensis strain B115 and containing at least 20 cytoplasmic proteins. These proteins were separated by SDS-PAGE. The unstained gel was divided into 18 fractions and proteins were eluted from the gel fractions. The capacity of the separated proteins to elicit delayed-type hypersensitivity (DTH) in infected guinea-pigs or to induce the production of interferon-γ (IFN-γ) by blood cells from infected cattle was evaluated. The biological activity of the corresponding protein fractions blotted on to nitrocellulose was measured in a lymphocyte blastogenesis assay. Among the 18 fractions tested, two - spanning the mol. wt ranges 17-22 (fraction 8) and 35-42-kDa (fraction 17) - showed the maximum biological activity in the three tests. These fractions contain two antigens, the Brucella bacterioferritin (BFR) and P39 proteins. Both proteins are good candidates for the detection of cellular immunity to Brucella.

Original language	English
Pages (from-to)	238-242
Number of pages	5
Journal	Journal of medical microbiology
Volume	46
Issue number	9
DOIs	https://doi.org/10.1099/00222615-46-9-801
Publication status	Published - 1997

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10.1099/00222615-46-9-801

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title = "Identification of the major T-cell antigens present in the Brucella melitensis B115 protein preparation, brucellergene OCB",

abstract = "Brucellergene is a commercial allergen prepared from Brucella melitensis strain B115 and containing at least 20 cytoplasmic proteins. These proteins were separated by SDS-PAGE. The unstained gel was divided into 18 fractions and proteins were eluted from the gel fractions. The capacity of the separated proteins to elicit delayed-type hypersensitivity (DTH) in infected guinea-pigs or to induce the production of interferon-γ (IFN-γ) by blood cells from infected cattle was evaluated. The biological activity of the corresponding protein fractions blotted on to nitrocellulose was measured in a lymphocyte blastogenesis assay. Among the 18 fractions tested, two - spanning the mol. wt ranges 17-22 (fraction 8) and 35-42-kDa (fraction 17) - showed the maximum biological activity in the three tests. These fractions contain two antigens, the Brucella bacterioferritin (BFR) and P39 proteins. Both proteins are good candidates for the detection of cellular immunity to Brucella.",

author = "Philippe Denoel and T.K.O. Vo and Vincent Weynants and Anne Tibor and Dominique Gilson and Michel Zygmunt and Joseph Limet and Jean-Jacques Letesson",

year = "1997",

doi = "10.1099/00222615-46-9-801",

language = "English",

volume = "46",

pages = "238--242",

journal = "Journal of medical microbiology",

issn = "1473-5644",

publisher = "Microbiology Society",

number = "9",

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TY - JOUR

T1 - Identification of the major T-cell antigens present in the Brucella melitensis B115 protein preparation, brucellergene OCB

AU - Denoel, Philippe

AU - Vo, T.K.O.

AU - Weynants, Vincent

AU - Tibor, Anne

AU - Gilson, Dominique

AU - Zygmunt, Michel

AU - Limet, Joseph

AU - Letesson, Jean-Jacques

PY - 1997

Y1 - 1997

N2 - Brucellergene is a commercial allergen prepared from Brucella melitensis strain B115 and containing at least 20 cytoplasmic proteins. These proteins were separated by SDS-PAGE. The unstained gel was divided into 18 fractions and proteins were eluted from the gel fractions. The capacity of the separated proteins to elicit delayed-type hypersensitivity (DTH) in infected guinea-pigs or to induce the production of interferon-γ (IFN-γ) by blood cells from infected cattle was evaluated. The biological activity of the corresponding protein fractions blotted on to nitrocellulose was measured in a lymphocyte blastogenesis assay. Among the 18 fractions tested, two - spanning the mol. wt ranges 17-22 (fraction 8) and 35-42-kDa (fraction 17) - showed the maximum biological activity in the three tests. These fractions contain two antigens, the Brucella bacterioferritin (BFR) and P39 proteins. Both proteins are good candidates for the detection of cellular immunity to Brucella.

AB - Brucellergene is a commercial allergen prepared from Brucella melitensis strain B115 and containing at least 20 cytoplasmic proteins. These proteins were separated by SDS-PAGE. The unstained gel was divided into 18 fractions and proteins were eluted from the gel fractions. The capacity of the separated proteins to elicit delayed-type hypersensitivity (DTH) in infected guinea-pigs or to induce the production of interferon-γ (IFN-γ) by blood cells from infected cattle was evaluated. The biological activity of the corresponding protein fractions blotted on to nitrocellulose was measured in a lymphocyte blastogenesis assay. Among the 18 fractions tested, two - spanning the mol. wt ranges 17-22 (fraction 8) and 35-42-kDa (fraction 17) - showed the maximum biological activity in the three tests. These fractions contain two antigens, the Brucella bacterioferritin (BFR) and P39 proteins. Both proteins are good candidates for the detection of cellular immunity to Brucella.

U2 - 10.1099/00222615-46-9-801

DO - 10.1099/00222615-46-9-801

M3 - Article

SN - 1473-5644

VL - 46

SP - 238

EP - 242

JO - Journal of medical microbiology

JF - Journal of medical microbiology

IS - 9

ER -

Identification of the major T-cell antigens present in the Brucella melitensis B115 protein preparation, brucellergene OCB

Abstract

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