Identification of p38MAPK-dependent genes with changed transcript abundance in H2O2-induced premature senescence of IMR-90 hTERT human fibroblasts

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Abstract

Premature senescence of IMR-90 human diploid fibroblasts expressing telomerase (hTERT) establishes after exposure to an acute sublethal concentration of H2O2. We showed herein that p38MAPK was phosphorylated after exposure of IMR-90 hTERT cells to H2O2. Selective inhibition of p38MAPK activity attenuated the increase in the proportion of cells positive for senescence associated β-galactosidase activity. We generated a low density DNA array to study gene expression profiles of 240 senescence-related genes. Using this array, p38MAPK inhibitor and p38MAPK small interferent RNA, we identified several p38MAPK-target genes differentially expressed in H2O2-stressed IMR-90 hTERT fibroblasts. © 2006 Federation of European Biochemical Societies.

Original languageEnglish
Pages (from-to)6455-6463
Number of pages9
JournalFEBS Letters
Volume580
Issue number27
DOIs
Publication statusPublished - 27 Nov 2006

Keywords

  • Fibroblasts
  • Gene expression
  • Oxidative stress
  • p38
  • Senescence
  • Telomerase

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