Identification of p38MAPK-dependent genes with changed transcript abundance in H2O2-induced premature senescence of IMR-90 hTERT human fibroblasts

Stéphanie Zdanov; Florence Debacq-Chainiaux; José Remacle; Olivier Toussaint

doi:10.1016/j.febslet.2006.10.064

Identification of p38^MAPK-dependent genes with changed transcript abundance in H₂O₂-induced premature senescence of IMR-90 hTERT human fibroblasts

Stéphanie Zdanov, Florence Debacq-Chainiaux, José Remacle, Olivier Toussaint

Research output: Contribution to journal › Article › peer-review

Abstract

Premature senescence of IMR-90 human diploid fibroblasts expressing telomerase (hTERT) establishes after exposure to an acute sublethal concentration of H₂O₂. We showed herein that p38^MAPK was phosphorylated after exposure of IMR-90 hTERT cells to H₂O₂. Selective inhibition of p38^MAPK activity attenuated the increase in the proportion of cells positive for senescence associated β-galactosidase activity. We generated a low density DNA array to study gene expression profiles of 240 senescence-related genes. Using this array, p38^MAPK inhibitor and p38^MAPK small interferent RNA, we identified several p38^MAPK-target genes differentially expressed in H₂O₂-stressed IMR-90 hTERT fibroblasts. © 2006 Federation of European Biochemical Societies.

Original language	English
Pages (from-to)	6455-6463
Number of pages	9
Journal	FEBS Letters
Volume	580
Issue number	27
DOIs	https://doi.org/10.1016/j.febslet.2006.10.064
Publication status	Published - 27 Nov 2006

Keywords

Fibroblasts
Gene expression
Oxidative stress
p38
Senescence
Telomerase

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10.1016/j.febslet.2006.10.064

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title = "Identification of p38MAPK-dependent genes with changed transcript abundance in H2O2-induced premature senescence of IMR-90 hTERT human fibroblasts",

abstract = "Premature senescence of IMR-90 human diploid fibroblasts expressing telomerase (hTERT) establishes after exposure to an acute sublethal concentration of H2O2. We showed herein that p38MAPK was phosphorylated after exposure of IMR-90 hTERT cells to H2O2. Selective inhibition of p38MAPK activity attenuated the increase in the proportion of cells positive for senescence associated β-galactosidase activity. We generated a low density DNA array to study gene expression profiles of 240 senescence-related genes. Using this array, p38MAPK inhibitor and p38MAPK small interferent RNA, we identified several p38MAPK-target genes differentially expressed in H2O2-stressed IMR-90 hTERT fibroblasts. {\textcopyright} 2006 Federation of European Biochemical Societies.",

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T1 - Identification of p38MAPK-dependent genes with changed transcript abundance in H2O2-induced premature senescence of IMR-90 hTERT human fibroblasts

AU - Zdanov, Stéphanie

AU - Debacq-Chainiaux, Florence

AU - Remacle, José

AU - Toussaint, Olivier

PY - 2006/11/27

Y1 - 2006/11/27

N2 - Premature senescence of IMR-90 human diploid fibroblasts expressing telomerase (hTERT) establishes after exposure to an acute sublethal concentration of H2O2. We showed herein that p38MAPK was phosphorylated after exposure of IMR-90 hTERT cells to H2O2. Selective inhibition of p38MAPK activity attenuated the increase in the proportion of cells positive for senescence associated β-galactosidase activity. We generated a low density DNA array to study gene expression profiles of 240 senescence-related genes. Using this array, p38MAPK inhibitor and p38MAPK small interferent RNA, we identified several p38MAPK-target genes differentially expressed in H2O2-stressed IMR-90 hTERT fibroblasts. © 2006 Federation of European Biochemical Societies.

AB - Premature senescence of IMR-90 human diploid fibroblasts expressing telomerase (hTERT) establishes after exposure to an acute sublethal concentration of H2O2. We showed herein that p38MAPK was phosphorylated after exposure of IMR-90 hTERT cells to H2O2. Selective inhibition of p38MAPK activity attenuated the increase in the proportion of cells positive for senescence associated β-galactosidase activity. We generated a low density DNA array to study gene expression profiles of 240 senescence-related genes. Using this array, p38MAPK inhibitor and p38MAPK small interferent RNA, we identified several p38MAPK-target genes differentially expressed in H2O2-stressed IMR-90 hTERT fibroblasts. © 2006 Federation of European Biochemical Societies.

KW - Fibroblasts

KW - Gene expression

KW - Oxidative stress

KW - p38

KW - Senescence

KW - Telomerase

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DO - 10.1016/j.febslet.2006.10.064

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JO - FEBS Letters

JF - FEBS Letters

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Identification of p38^MAPK-dependent genes with changed transcript abundance in H₂O₂-induced premature senescence of IMR-90 hTERT human fibroblasts

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Keywords

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