Abstract
A rhodamine-cyanobenzene conjugate, (E)-4-((2-(3',6'-bis(diethylamino)-3-oxospiro[isoindoline-1,9'-xanthene]-2-yl)ethylimino)methyl)benzonitrile (1), which structure has been elucidated by single crystal X-ray diffraction, was synthesized for selective fluorescent "turn-on" and colorimetric recognition of hydrazine at physiological pH 7.4. It was established that 1 detects hydrazine up to 58 nM. The probe is useful for the detection of intracellular hydrazine in the human breast cancer cells MCF-7 using a fluorescence microscope. Spirolactam ring opening of 1, followed by its hydrolysis, was established as a probable mechanism for the selective sensing of hydrazine.
Original language | English |
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Pages (from-to) | 84-90 |
Number of pages | 7 |
Journal | Analytica chimica acta |
Volume | 893 |
DOIs | |
Publication status | Published - 2015 |
Externally published | Yes |
Keywords
- "Turn-on" fluorescence
- Cell imaging
- Chemodosimetric probe
- Hydrazine
- Rhodamine
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CCDC 995593: Experimental Crystal Structure Determination
Nandi, S. (Contributor), Sahana, A. (Contributor), Mandal, S. (Contributor), Sengupta, A. (Contributor), Chatterjee, A. (Contributor), Safin, D. A. (Contributor), Babashkina, M. G. (Contributor), Tumanov, N. (Contributor), Filinchuk, Y. (Contributor) & Das, D. (Contributor), Cambridge Crystallographic Data Centre, 1 Jan 2015
DOI: 10.5517/cc12dzxc, http://www.ccdc.cam.ac.uk/services/structure_request?id=doi:10.5517/cc12dzxc&sid=DataCite
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