Projects per year
Abstract
Brucella spp are intracellular bacteria that cause brucellosis, one of the most common zoonoses in the world. Given the serious medical consequences of this disease, a safe and effective human vaccine is urgently needed. Efforts to develop this vaccine have been hampered by our lack of understanding of what constitutes a protective memory response against Brucella. In this study, we characterize the cells and signaling pathways implicated in the generation of a protective immune memory response following priming by the injection of heat-killed or live Brucella melitensis 16M. Using a panel of gene-deficient mice, we demonstrated that during a secondary recall response, both the Brucella-specific humoral response and CD4(+) Th1 cells must act together to confer protective immunity in the spleen to B. melitensis infection. Humoral protective immunity is induced by the inoculation of both heat-killed and live bacteria, and its development does not require T cells, MyD88/IL-12p35 signaling pathways, or an activation-induced deaminase-mediated isotype switch. In striking contrast, the presence of memory IFN-γ-producing CD4(+) Th1 cells requires the administration of live bacteria and functional MyD88/IL-12p35 pathways. In summary, our work identifies several immune markers closely associated with protective immune memory and could help to define a rational strategy to obtain an effective human vaccine against brucellosis.
Original language | English |
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Pages (from-to) | 3740-52 |
Number of pages | 13 |
Journal | Journal of immunology |
Volume | 192 |
Issue number | 8 |
DOIs | |
Publication status | Published - 2014 |
Fingerprint Dive into the research topics of 'Humoral Immunity and CD4+ Th1 Cells Are Both Necessary for a Fully Protective Immune Response upon Secondary Infection with Brucella melitensis'. Together they form a unique fingerprint.
Projects
- 1 Finished
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Identifications in vivo des étapes du cycle infectieux de Brucella melitensis impliquées dans le développement d'une immunité protectrice chez la souris
LETESSON, J. & Hanot-Mambres, D.
1/10/10 → 30/09/12
Project: PHD
Equipment
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FACS
Charles Nicaise (Manager) & Francesca Cecchet (Manager)
Technological Platform Morphology - ImagingFacility/equipment: Equipment
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Morphology - Imaging
Charles Nicaise (Manager) & Francesca Cecchet (Manager)
Technological Platform Morphology - ImagingFacility/equipment: Technological Platform
Student Theses
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On the components of adaptive immune response involved in the control of a primary and secondary infection by Brucella melitensis
Author: Vitry, M., 15 Mar 2013Supervisor: Letesson, J. (Supervisor), Muraille, É. (Co-Supervisor), Michiels, C. (President), Leo, O. (External person) (Jury), Goddeeris, B. (External person) (Jury) & Baldwin, C. (External person) (Jury)
Student thesis: Doc types › Doctor of Sciences