Abstract

In this work, the evolution of some key physicochemical parameters of oxide engineered nanomaterial (ENM) dispersions was studied during an in vitro biological assessment. Commercial oxide ENMs, SiO and TiO , were dispersed in aqueous solutions (20 μg/mL) to A549 cells and N-hTERT keratinocytes and were assessed at several incubation times: 6, 24, 48, and 72 hours. The ENMs deposited dose and its particle size distribution (PSD) were followed each time. Centrifuge Liquid Sedimentation (CLS) measured the PSD and the ENMs deposited dose from a particle entity perspective, while Particle-Induced X-ray Emission (PIXE) measured the ENMs deposited dose from an elemental mass perspective. No significant variations in PSD were observed for SiO ENMs during incubation in A549 cells and TiO ENMs in both cell lines, while a continuous evolution of the PSD is observed for SiO in N-hTERT keratinocytes. The deposited dose for TiO ENMs remained stable and similar in both cell lines due to a smaller specific surface area and a higher quantity of primary particles present during incubation. It is concluded that the observed differences in the deposited dose are related to an interaction between the proteins present in the media and the ENMs specific surface.
Original languageEnglish
Article number012013
Number of pages10
JournalJournal of Physics : Conference Series
Volume429
Issue number1
DOIs
Publication statusPublished - 2013

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particle size distribution
dosage
oxides
cultured cells
centrifuges
cells
aqueous solutions
proteins
liquids
x rays
interactions

Cite this

@article{5dd068a2fcd3466caa190d7882957088,
title = "How does the deposited dose of oxide nanomaterials evolve in an in vitro assay?",
abstract = "In this work, the evolution of some key physicochemical parameters of oxide engineered nanomaterial (ENM) dispersions was studied during an in vitro biological assessment. Commercial oxide ENMs, SiO and TiO , were dispersed in aqueous solutions (20 μg/mL) to A549 cells and N-hTERT keratinocytes and were assessed at several incubation times: 6, 24, 48, and 72 hours. The ENMs deposited dose and its particle size distribution (PSD) were followed each time. Centrifuge Liquid Sedimentation (CLS) measured the PSD and the ENMs deposited dose from a particle entity perspective, while Particle-Induced X-ray Emission (PIXE) measured the ENMs deposited dose from an elemental mass perspective. No significant variations in PSD were observed for SiO ENMs during incubation in A549 cells and TiO ENMs in both cell lines, while a continuous evolution of the PSD is observed for SiO in N-hTERT keratinocytes. The deposited dose for TiO ENMs remained stable and similar in both cell lines due to a smaller specific surface area and a higher quantity of primary particles present during incubation. It is concluded that the observed differences in the deposited dose are related to an interaction between the proteins present in the media and the ENMs specific surface.",
author = "{Lozano Garcia}, Omar and {Mejia Mendoza}, {Jorge Humberto} and Jean-Pascal Piret and Christelle Saout and Jean-Michel Dogne and Olivier Toussaint and St{\'e}phane Lucas",
year = "2013",
doi = "10.1088/1742-6596/429/1/012013",
language = "English",
volume = "429",
journal = "Journal of Physics : Conference Series",
issn = "1742-6588",
publisher = "IOP Publishing Ltd.",
number = "1",

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TY - JOUR

T1 - How does the deposited dose of oxide nanomaterials evolve in an in vitro assay?

AU - Lozano Garcia, Omar

AU - Mejia Mendoza, Jorge Humberto

AU - Piret, Jean-Pascal

AU - Saout, Christelle

AU - Dogne, Jean-Michel

AU - Toussaint, Olivier

AU - Lucas, Stéphane

PY - 2013

Y1 - 2013

N2 - In this work, the evolution of some key physicochemical parameters of oxide engineered nanomaterial (ENM) dispersions was studied during an in vitro biological assessment. Commercial oxide ENMs, SiO and TiO , were dispersed in aqueous solutions (20 μg/mL) to A549 cells and N-hTERT keratinocytes and were assessed at several incubation times: 6, 24, 48, and 72 hours. The ENMs deposited dose and its particle size distribution (PSD) were followed each time. Centrifuge Liquid Sedimentation (CLS) measured the PSD and the ENMs deposited dose from a particle entity perspective, while Particle-Induced X-ray Emission (PIXE) measured the ENMs deposited dose from an elemental mass perspective. No significant variations in PSD were observed for SiO ENMs during incubation in A549 cells and TiO ENMs in both cell lines, while a continuous evolution of the PSD is observed for SiO in N-hTERT keratinocytes. The deposited dose for TiO ENMs remained stable and similar in both cell lines due to a smaller specific surface area and a higher quantity of primary particles present during incubation. It is concluded that the observed differences in the deposited dose are related to an interaction between the proteins present in the media and the ENMs specific surface.

AB - In this work, the evolution of some key physicochemical parameters of oxide engineered nanomaterial (ENM) dispersions was studied during an in vitro biological assessment. Commercial oxide ENMs, SiO and TiO , were dispersed in aqueous solutions (20 μg/mL) to A549 cells and N-hTERT keratinocytes and were assessed at several incubation times: 6, 24, 48, and 72 hours. The ENMs deposited dose and its particle size distribution (PSD) were followed each time. Centrifuge Liquid Sedimentation (CLS) measured the PSD and the ENMs deposited dose from a particle entity perspective, while Particle-Induced X-ray Emission (PIXE) measured the ENMs deposited dose from an elemental mass perspective. No significant variations in PSD were observed for SiO ENMs during incubation in A549 cells and TiO ENMs in both cell lines, while a continuous evolution of the PSD is observed for SiO in N-hTERT keratinocytes. The deposited dose for TiO ENMs remained stable and similar in both cell lines due to a smaller specific surface area and a higher quantity of primary particles present during incubation. It is concluded that the observed differences in the deposited dose are related to an interaction between the proteins present in the media and the ENMs specific surface.

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U2 - 10.1088/1742-6596/429/1/012013

DO - 10.1088/1742-6596/429/1/012013

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JO - Journal of Physics : Conference Series

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