Abstract
Acute repeated exposures to subcytotoxic concentrations of tert-butylhydroperoxide and ethanol trigger premature senescence of human diploid fibroblasts. In the present work we found an increased mRNA and protein level of interleukin-11 and heme oxygenase-1 in premature senescence of WI-38 human diploid foetal lung fibroblasts induced by both tert-butylhydroperoxide and ethanol. We tested whether interleukin-11 and heme oxygenase-1 could protect against tert-butylhydroperoxide- or ethanol-induced premature senescence when stable overexpression was established using a retroviral vector-based transduction. No protective effect was found against the decrease of the proliferative potential, the increase of the proportion of senescence-associated β-galactosidase positive cells and the increase of the mRNA levels of six senescence-associated genes. © Springer Science+Business Media, Inc. 2007.
Original language | English |
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Pages (from-to) | 409-422 |
Number of pages | 14 |
Journal | Biogerontology |
Volume | 8 |
Issue number | 4 |
DOIs | |
Publication status | Published - 1 Aug 2007 |
Keywords
- Cellular senescence
- Ethanol
- Heme oxygenase
- Human fibroblasts
- Interleukin-11
- Oxidative stress