Growth kinetics rather than stress accelerate telomere shortening in cultures of human diploid fibroblasts in oxidative stress-induced premature senescence

P. Dumont; V. Royer; T. Pascal; J.-F. Dierick; F. Chainiaux; C. Frippiat; J.P. De Magalhaes; F. Eliaers; J. Remacle; O. Toussaint

doi:10.1016/S0014-5793(01)02679-5

Growth kinetics rather than stress accelerate telomere shortening in cultures of human diploid fibroblasts in oxidative stress-induced premature senescence

P. Dumont, V. Royer, T. Pascal, J.-F. Dierick, F. Chainiaux, C. Frippiat, J.P. De Magalhaes, F. Eliaers, J. Remacle, O. Toussaint

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Abstract

WI-38 human diploid fibroblasts underwent accelerated telomere shortening (490 bp/stress) and growth arrest after exposure to four subcytotoxic 100 μM tert-butylhydroperoxide (t-BHP) stresses, with a stress at every two population doublings (PD). After subcytotoxic 160 μM HO stress or five repeated 30 μM t-BHP stresses along the same PD, respectively a 322±55 and 380±129 bp telomere shortening was observed only during the first PD after stress. The percentage of cells resuming proliferation after stress suggests this telomere shortening is due to the number of cell divisions accomplished to reach confluence during the first PD after stress.

Original language	English
Pages (from-to)	109-112
Number of pages	4
Journal	FEBS Letters
Volume	502
Issue number	3
DOIs	https://doi.org/10.1016/S0014-5793(01)02679-5
Publication status	Published - 3 Aug 2001

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Dumont, P., Royer, V., Pascal, T., Dierick, J.-F., Chainiaux, F., Frippiat, C., De Magalhaes, J. P., Eliaers, F., Remacle, J., & Toussaint, O. (2001). Growth kinetics rather than stress accelerate telomere shortening in cultures of human diploid fibroblasts in oxidative stress-induced premature senescence. FEBS Letters, 502(3), 109-112. https://doi.org/10.1016/S0014-5793(01)02679-5

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title = "Growth kinetics rather than stress accelerate telomere shortening in cultures of human diploid fibroblasts in oxidative stress-induced premature senescence",

abstract = "WI-38 human diploid fibroblasts underwent accelerated telomere shortening (490 bp/stress) and growth arrest after exposure to four subcytotoxic 100 μM tert-butylhydroperoxide (t-BHP) stresses, with a stress at every two population doublings (PD). After subcytotoxic 160 μM HO stress or five repeated 30 μM t-BHP stresses along the same PD, respectively a 322±55 and 380±129 bp telomere shortening was observed only during the first PD after stress. The percentage of cells resuming proliferation after stress suggests this telomere shortening is due to the number of cell divisions accomplished to reach confluence during the first PD after stress.",

author = "P. Dumont and V. Royer and T. Pascal and J.-F. Dierick and F. Chainiaux and C. Frippiat and {De Magalhaes}, J.P. and F. Eliaers and J. Remacle and O. Toussaint",

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doi = "10.1016/S0014-5793(01)02679-5",

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Dumont, P, Royer, V, Pascal, T, Dierick, J-F, Chainiaux, F, Frippiat, C, De Magalhaes, JP, Eliaers, F, Remacle, J & Toussaint, O 2001, 'Growth kinetics rather than stress accelerate telomere shortening in cultures of human diploid fibroblasts in oxidative stress-induced premature senescence', FEBS Letters, vol. 502, no. 3, pp. 109-112. https://doi.org/10.1016/S0014-5793(01)02679-5

TY - JOUR

T1 - Growth kinetics rather than stress accelerate telomere shortening in cultures of human diploid fibroblasts in oxidative stress-induced premature senescence

AU - Dumont, P.

AU - Royer, V.

AU - Pascal, T.

AU - Dierick, J.-F.

AU - Chainiaux, F.

AU - Frippiat, C.

AU - De Magalhaes, J.P.

AU - Eliaers, F.

AU - Remacle, J.

AU - Toussaint, O.

PY - 2001/8/3

Y1 - 2001/8/3

N2 - WI-38 human diploid fibroblasts underwent accelerated telomere shortening (490 bp/stress) and growth arrest after exposure to four subcytotoxic 100 μM tert-butylhydroperoxide (t-BHP) stresses, with a stress at every two population doublings (PD). After subcytotoxic 160 μM HO stress or five repeated 30 μM t-BHP stresses along the same PD, respectively a 322±55 and 380±129 bp telomere shortening was observed only during the first PD after stress. The percentage of cells resuming proliferation after stress suggests this telomere shortening is due to the number of cell divisions accomplished to reach confluence during the first PD after stress.

AB - WI-38 human diploid fibroblasts underwent accelerated telomere shortening (490 bp/stress) and growth arrest after exposure to four subcytotoxic 100 μM tert-butylhydroperoxide (t-BHP) stresses, with a stress at every two population doublings (PD). After subcytotoxic 160 μM HO stress or five repeated 30 μM t-BHP stresses along the same PD, respectively a 322±55 and 380±129 bp telomere shortening was observed only during the first PD after stress. The percentage of cells resuming proliferation after stress suggests this telomere shortening is due to the number of cell divisions accomplished to reach confluence during the first PD after stress.

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U2 - 10.1016/S0014-5793(01)02679-5

DO - 10.1016/S0014-5793(01)02679-5

M3 - Article

AN - SCOPUS:0035800610

SN - 0014-5793

VL - 502

SP - 109

EP - 112

JO - FEBS Letters

JF - FEBS Letters

IS - 3

ER -

Growth kinetics rather than stress accelerate telomere shortening in cultures of human diploid fibroblasts in oxidative stress-induced premature senescence

Abstract

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