Gateway to genetic exchange? DNA double-strand breaks in the bdelloid rotifer Adineta vaga submitted to desiccation

B. Hespeels, M. Knapen, D. Hanot-Mambres, A.-C. Heuskin, F. Pineux, S. Lucas, R. Koszul, K. Van Doninck

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Abstract

The bdelloid rotifer lineage Adineta vaga inhabits temporary habitats subjected to frequent episodes of drought. The recently published draft sequence of the genome of A. vaga revealed a peculiar genomic structure incompatible with meiosis and suggesting that DNA damage induced by desiccation may have reshaped the genomic structure of these organisms. However, the causative link between DNA damage and desiccation has never been proven to date in rotifers. To test for the hypothesis that desiccation induces DNA double-strand breaks (DSBs), we developed a protocol allowing a high survival rate of desiccated A. vaga. Using pulsed-field gel electrophoresis to monitor genomic integrity, we followed the occurrence of DSBs in dried bdelloids and observed an accumulation of these breaks with time spent in dehydrated state. These DSBs are gradually repaired upon rehydration. Even when the genome was entirely shattered into small DNA fragments by proton radiation, A. vaga individuals were able to efficiently recover from desiccation and repair a large amount of DSBs. Interestingly, when investigating the influence of UV-A and UV-B exposure on the genomic integrity of desiccated bdelloids, we observed that these natural radiations also caused important DNA DSBs, suggesting that the genome is not protected during the desiccated stage but that the repair mechanisms are extremely efficient in these intriguing organisms.
Original languageEnglish
Article number27(7)
Pages (from-to)1334-1345
JournalJournal of Evolutionary Biology
DOIs
Publication statusPublished - 15 Feb 2014

Keywords

  • anhydrobiosis, asexual reproduction, DNA damage, DNA repair, horizontal gene transfer, pulsed-field gel electrophoresis, UV radiation

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    International Conference "Radiation Damage in Biomolecular Systems: Nanoscale Insights into Ion-Beam Cancer Therapy" (Nano-IBCT 2014)

    Stéphane Lucas (Speaker)

    27 Oct 201431 Oct 2014

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    2nd BHTC workshop ; Hadrontherapy: from cell to patient

    Stéphane Lucas (Organiser)

    23 May 2014

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