Projects per year
Abstract
Several intracellular pathogens, such as Brucella abortus, display a biphasic infection process starting with a non-proliferative stage of unclear nature. Here, we study the cell cycle of B. abortus at the single-cell level, in culture and during infection of HeLa cells and macrophages. The localization of segregation and replication loci of the two bacterial chromosomes indicates that, immediately after being engulfed by host-cell endocytic vacuoles, most bacterial cells are newborn. These bacterial cells do not initiate DNA replication for the next 4 to 6 h, indicating a G1 arrest. Moreover, growth is completely stopped during that time, reflecting a global cell cycle block. Growth and DNA replication resume later, although bacteria still reside within endosomal-like compartments. We hypothesize that the predominance of G1-arrested bacteria in the infectious population, and the bacterial cell cycle arrest following internalization, may constitute a widespread strategy among intracellular pathogens to colonize new proliferation niches.
Original language | English |
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Pages (from-to) | 4366 |
Number of pages | 11 |
Journal | Nature Communications |
Volume | 5 |
DOIs | |
Publication status | Published - 2014 |
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Dive into the research topics of 'G1-arrested newborn cells are the predominant infectious form of the pathogen Brucella abortus'. Together they form a unique fingerprint.Projects
- 3 Finished
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Mécanismes de contrôle du cycle cellulaire de Brucella abortus en cours d'infection
1/07/13 → 30/06/15
Project: Research
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PAI n°P7/28 - MICRODEV: PAI n°P7/28 - MICRODEV: Role of developmental processes in the virulence of human pathogens : from molecular mechanisms to novel therapeutic targets
LETESSON, J., Coenye, T., Van Dijck, P., Van Melderen, L., HOLS, P. & De Bolle, X.
1/10/12 → 30/09/17
Project: Research
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ARC: Identification and characterization of bacterial effectors using a post-genomic approach
De Bolle, X. & FILOPON, D.
1/01/05 → 31/12/08
Project: Research
Equipment
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Biological Security Laboratory Level 3 (BL3)
Xavier De Bolle (Manager)
Technological Platform: Biological Security Laboratory Level 3Facility/equipment: Technological Platform
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Confocal microscope
Charles Nicaise (Manager) & Francesca Cecchet (Manager)
Technological Platform Morphology - ImagingFacility/equipment: Equipment
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FACS
Charles Nicaise (Manager) & Francesca Cecchet (Manager)
Technological Platform Morphology - ImagingFacility/equipment: Equipment
Student theses
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Connecting the cell cycle and the pathogenicity of Brucella abortus. When a specific bacterial cell cycle arrest in G1 phase takes part in the colonization of mammalian cells
Author: Deghelt, M., 16 Dec 2013Supervisor: De Bolle, X. (Supervisor), Letesson, J. (Supervisor), Muylkens, B. (Jury), Van Doninck, K. (President), THANBICHLER, M. (External person) (Jury) & ROOP II, R. M. (External person) (Jury)
Student thesis: Doc types › Doctor of Sciences
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On the link between Brucella abortus cell cycle and adaptation to its intracellular lifestyle
Author: Mullier, C., 24 Jan 2014Supervisor: De Bolle, X. (Supervisor), Renard, P. (President), Jadot, M. (Jury), Matroule, J. (Jury), Salcedo, S. P. (External person) (Jury) & Collet, J. (External person) (Jury)
Student thesis: Doc types › Doctor of Sciences