G1-arrested newborn cells are the predominant infectious form of the pathogen Brucella abortus

Michaël Deghelt, Caroline Mullier, Jean-François Sternon, Nayla Francis, Géraldine Laloux, Delphine Dotreppe, Charles Van der Henst, Christine Jacobs-Wagner, Jean-Jacques Letesson, Xavier De Bolle

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Abstract

Several intracellular pathogens, such as Brucella abortus, display a biphasic infection process starting with a non-proliferative stage of unclear nature. Here, we study the cell cycle of B. abortus at the single-cell level, in culture and during infection of HeLa cells and macrophages. The localization of segregation and replication loci of the two bacterial chromosomes indicates that, immediately after being engulfed by host-cell endocytic vacuoles, most bacterial cells are newborn. These bacterial cells do not initiate DNA replication for the next 4 to 6 h, indicating a G1 arrest. Moreover, growth is completely stopped during that time, reflecting a global cell cycle block. Growth and DNA replication resume later, although bacteria still reside within endosomal-like compartments. We hypothesize that the predominance of G1-arrested bacteria in the infectious population, and the bacterial cell cycle arrest following internalization, may constitute a widespread strategy among intracellular pathogens to colonize new proliferation niches.

Original languageEnglish
Pages (from-to)4366
Number of pages11
JournalNature Communications
Volume5
DOIs
Publication statusPublished - 2014

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    Connecting the cell cycle and the pathogenicity of Brucella abortus. When a specific bacterial cell cycle arrest in G1 phase takes part in the colonization of mammalian cells

    Author: Deghelt, M., 16 Dec 2013

    Supervisor: De Bolle, X. (Supervisor), Letesson, J. (Supervisor), Muylkens, B. (Jury), Van Doninck, K. (President), THANBICHLER, M. (External person) (Jury) & ROOP II, R. M. (External person) (Jury)

    Student thesis: Doc typesDoctor of Sciences

    On the link between Brucella abortus cell cycle and adaptation to its intracellular lifestyle

    Author: Mullier, C., 24 Jan 2014

    Supervisor: De Bolle, X. (Supervisor), Renard, P. (President), Jadot, M. (Jury), Matroule, J. (Jury), Salcedo, S. P. (External person) (Jury) & Collet, J. (External person) (Jury)

    Student thesis: Doc typesDoctor of Sciences

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