Abstract
A rapid and accurate diagnosis in patients with suspected heparin-induced thrombocytopenia (HIT) is essential for patient management but remains challenging. Current HIT diagnosis ideally relies on a combination of clinical information, immunoassay and functional assay results. Platelet activation assays or functional assays detect HIT antibodies that are more clinically significant. Several functional assays have been developed and evaluated in the literature. They differ in the activation endpoint studied; the technique or technology used; the platelet donor selection; the platelet suspension (washed platelets, platelet rich plasma or whole blood); the patient sample (serum or plasma); and the heparin used (type and concentrations). Inconsistencies in controls performed and associated results interpretation are common. Thresholds and performances are determined differently among papers. Functional assays suffer from interlaboratory variability. This lack of standardization limits the evaluation and the accessibility of functional assays in laboratories. In the present article, we review all the current activation endpoints, techniques and methodologies of functional assays developed for HIT diagnosis.
| Original language | English |
|---|---|
| Article number | 617 |
| Journal | Molecules |
| Volume | 22 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - 1 Apr 2017 |
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Keywords
- Diagnosis
- Functional assay
- Heparin-induced thrombocytopenia
- Platelets
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Functional assays in the diagnosis of heparin-induced thrombocytopenia : A review. / Minet, Valentine; Dogné, Jean Michel; Mullier, François.
In: Molecules, Vol. 22, No. 4, 617, 01.04.2017.Research output: Contribution to journal › Review article
TY - JOUR
T1 - Functional assays in the diagnosis of heparin-induced thrombocytopenia
T2 - A review
AU - Minet, Valentine
AU - Dogné, Jean Michel
AU - Mullier, François
PY - 2017/4/1
Y1 - 2017/4/1
N2 - A rapid and accurate diagnosis in patients with suspected heparin-induced thrombocytopenia (HIT) is essential for patient management but remains challenging. Current HIT diagnosis ideally relies on a combination of clinical information, immunoassay and functional assay results. Platelet activation assays or functional assays detect HIT antibodies that are more clinically significant. Several functional assays have been developed and evaluated in the literature. They differ in the activation endpoint studied; the technique or technology used; the platelet donor selection; the platelet suspension (washed platelets, platelet rich plasma or whole blood); the patient sample (serum or plasma); and the heparin used (type and concentrations). Inconsistencies in controls performed and associated results interpretation are common. Thresholds and performances are determined differently among papers. Functional assays suffer from interlaboratory variability. This lack of standardization limits the evaluation and the accessibility of functional assays in laboratories. In the present article, we review all the current activation endpoints, techniques and methodologies of functional assays developed for HIT diagnosis.
AB - A rapid and accurate diagnosis in patients with suspected heparin-induced thrombocytopenia (HIT) is essential for patient management but remains challenging. Current HIT diagnosis ideally relies on a combination of clinical information, immunoassay and functional assay results. Platelet activation assays or functional assays detect HIT antibodies that are more clinically significant. Several functional assays have been developed and evaluated in the literature. They differ in the activation endpoint studied; the technique or technology used; the platelet donor selection; the platelet suspension (washed platelets, platelet rich plasma or whole blood); the patient sample (serum or plasma); and the heparin used (type and concentrations). Inconsistencies in controls performed and associated results interpretation are common. Thresholds and performances are determined differently among papers. Functional assays suffer from interlaboratory variability. This lack of standardization limits the evaluation and the accessibility of functional assays in laboratories. In the present article, we review all the current activation endpoints, techniques and methodologies of functional assays developed for HIT diagnosis.
KW - Diagnosis
KW - Functional assay
KW - Heparin-induced thrombocytopenia
KW - Platelets
UR - http://www.scopus.com/inward/record.url?scp=85019655394&partnerID=8YFLogxK
U2 - 10.3390/molecules22040617
DO - 10.3390/molecules22040617
M3 - Review article
C2 - 28398258
AN - SCOPUS:85019655394
VL - 22
JO - Molecules
JF - Molecules
SN - 1431-5165
IS - 4
M1 - 617
ER -