Functional assays in the diagnosis of heparin-induced thrombocytopenia: A review

Research output: Contribution to journalReview article

Abstract

A rapid and accurate diagnosis in patients with suspected heparin-induced thrombocytopenia (HIT) is essential for patient management but remains challenging. Current HIT diagnosis ideally relies on a combination of clinical information, immunoassay and functional assay results. Platelet activation assays or functional assays detect HIT antibodies that are more clinically significant. Several functional assays have been developed and evaluated in the literature. They differ in the activation endpoint studied; the technique or technology used; the platelet donor selection; the platelet suspension (washed platelets, platelet rich plasma or whole blood); the patient sample (serum or plasma); and the heparin used (type and concentrations). Inconsistencies in controls performed and associated results interpretation are common. Thresholds and performances are determined differently among papers. Functional assays suffer from interlaboratory variability. This lack of standardization limits the evaluation and the accessibility of functional assays in laboratories. In the present article, we review all the current activation endpoints, techniques and methodologies of functional assays developed for HIT diagnosis.

Original languageEnglish
Article number617
JournalMolecules
Volume22
Issue number4
DOIs
Publication statusPublished - 1 Apr 2017

Keywords

  • Diagnosis
  • Functional assay
  • Heparin-induced thrombocytopenia
  • Platelets

Fingerprint Dive into the research topics of 'Functional assays in the diagnosis of heparin-induced thrombocytopenia: A review'. Together they form a unique fingerprint.

  • Student Theses

    Analytical and clinical validation of sensitive assays for the diagnosis of immune heparin-induced thrombocytopenia

    Author: Minet, V., 2017

    Supervisor: Dogne, J. (Supervisor), Mullier, F. (External person) (Co-Supervisor), Laloy, J. (Jury), Lucas, S. (Jury), Masereel, B. (Jury), Chatelain, M. (External person) (Jury), Hoylaerts, M. F. (External person) (Jury) & Tardy, B. (External person) (Jury)

    Student thesis: Doc typesDoctor of Biomedical and Pharmaceutical Sciences

    Cite this