Folliculin regulates mTORC1/2 and WNT pathways in early human pluripotency

J. Mathieu, D. Detraux, D. Kuppers, Y. Wang, C. Cavanaugh, S. Sidhu, S. Levy, A. M. Robitaille, A. Ferreccio, T. Bottorff, A. McAlister, L. Somasundaram, F. Artoni, S. Battle, R. D. Hawkins, R. T. Moon, C. B. Ware, P. J. Paddison, H. Ruohola-Baker

Research output: Contribution to journalArticle

Abstract

To reveal how cells exit human pluripotency, we designed a CRISPR-Cas9 screen exploiting the metabolic and epigenetic differences between naïve and primed pluripotent cells. We identify the tumor suppressor, Folliculin(FLCN) as a critical gene required for the exit from human pluripotency. Here we show that FLCN Knock-out (KO) hESCs maintain the naïve pluripotent state but cannot exit the state since the critical transcription factor TFE3 remains active in the nucleus. TFE3 targets up-regulated in FLCN KO exit assay are members of Wnt pathway and ESRRB. Treatment of FLCN KO hESC with a Wnt inhibitor, but not ESRRB/FLCN double mutant, rescues the cells, allowing the exit from the naïve state. Using co-immunoprecipitation and mass spectrometry analysis we identify unique FLCN binding partners. The interactions of FLCN with components of the mTOR pathway (mTORC1 and mTORC2) reveal a mechanism of FLCN function during exit from naïve pluripotency.

Original languageEnglish
Article number632
JournalNature Communications
Volume10
Issue number1
DOIs
Publication statusPublished - 1 Dec 2019

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Estrone
cells
suppressors
Clustered Regularly Interspaced Short Palindromic Repeats
genes
inhibitors
Wnt Signaling Pathway
mechanistic target of rapamycin complex 1
mass spectroscopy
tumors
Immunoprecipitation
Epigenomics
Mass spectrometry
Tumors
Assays
Mass Spectrometry
nuclei
Transcription Factors
Genes
spectroscopy

Cite this

Mathieu, J. ; Detraux, D. ; Kuppers, D. ; Wang, Y. ; Cavanaugh, C. ; Sidhu, S. ; Levy, S. ; Robitaille, A. M. ; Ferreccio, A. ; Bottorff, T. ; McAlister, A. ; Somasundaram, L. ; Artoni, F. ; Battle, S. ; Hawkins, R. D. ; Moon, R. T. ; Ware, C. B. ; Paddison, P. J. ; Ruohola-Baker, H. / Folliculin regulates mTORC1/2 and WNT pathways in early human pluripotency. In: Nature Communications. 2019 ; Vol. 10, No. 1.
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Mathieu, J, Detraux, D, Kuppers, D, Wang, Y, Cavanaugh, C, Sidhu, S, Levy, S, Robitaille, AM, Ferreccio, A, Bottorff, T, McAlister, A, Somasundaram, L, Artoni, F, Battle, S, Hawkins, RD, Moon, RT, Ware, CB, Paddison, PJ & Ruohola-Baker, H 2019, 'Folliculin regulates mTORC1/2 and WNT pathways in early human pluripotency', Nature Communications, vol. 10, no. 1, 632. https://doi.org/10.1038/s41467-018-08020-0

Folliculin regulates mTORC1/2 and WNT pathways in early human pluripotency. / Mathieu, J.; Detraux, D.; Kuppers, D.; Wang, Y.; Cavanaugh, C.; Sidhu, S.; Levy, S.; Robitaille, A. M.; Ferreccio, A.; Bottorff, T.; McAlister, A.; Somasundaram, L.; Artoni, F.; Battle, S.; Hawkins, R. D.; Moon, R. T.; Ware, C. B.; Paddison, P. J.; Ruohola-Baker, H.

In: Nature Communications, Vol. 10, No. 1, 632, 01.12.2019.

Research output: Contribution to journalArticle

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AU - Mathieu, J.

AU - Detraux, D.

AU - Kuppers, D.

AU - Wang, Y.

AU - Cavanaugh, C.

AU - Sidhu, S.

AU - Levy, S.

AU - Robitaille, A. M.

AU - Ferreccio, A.

AU - Bottorff, T.

AU - McAlister, A.

AU - Somasundaram, L.

AU - Artoni, F.

AU - Battle, S.

AU - Hawkins, R. D.

AU - Moon, R. T.

AU - Ware, C. B.

AU - Paddison, P. J.

AU - Ruohola-Baker, H.

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Y1 - 2019/12/1

N2 - To reveal how cells exit human pluripotency, we designed a CRISPR-Cas9 screen exploiting the metabolic and epigenetic differences between naïve and primed pluripotent cells. We identify the tumor suppressor, Folliculin(FLCN) as a critical gene required for the exit from human pluripotency. Here we show that FLCN Knock-out (KO) hESCs maintain the naïve pluripotent state but cannot exit the state since the critical transcription factor TFE3 remains active in the nucleus. TFE3 targets up-regulated in FLCN KO exit assay are members of Wnt pathway and ESRRB. Treatment of FLCN KO hESC with a Wnt inhibitor, but not ESRRB/FLCN double mutant, rescues the cells, allowing the exit from the naïve state. Using co-immunoprecipitation and mass spectrometry analysis we identify unique FLCN binding partners. The interactions of FLCN with components of the mTOR pathway (mTORC1 and mTORC2) reveal a mechanism of FLCN function during exit from naïve pluripotency.

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