TY - JOUR
T1 - Exploring the interactions between polyunsaturated fatty acids and cadmium in rainbow trout liver cells: A genetic and proteomic study
AU - Ferain, Aline
AU - Bonnineau, Chloé
AU - Neefs, Ineke
AU - De Saeyer, Nancy
AU - Lemaire, Benjamin
AU - Cornet, Valérie
AU - Larondelle, Yvan
AU - De Schamphelaere, Karel
AU - Debier, Cathy
AU - Rees, Jean-François
N1 - Funding Information:
The research leading to these results has received funding from Belspo for the IAP AQUASTRESS project (P7/31) , from the European Union Seventh Framework Programme (FP7/2007-2013) under grant agreement PIEF-GA-2012-332049 for the FISHSTRESS project and from the Fonds de la Recherche Scientifique (FNRS) (Aline Ferain is a F.R.I.A. Grant Holder of the FNRS). The authors acknowledge Catherine Rasse, Charlotte Baugnée, Gilles Tinant, Emmy Pequeur, Jolien Depecker, Hervé Degand and Jennifer Villers for their contribution to this work.
Publisher Copyright:
© 2018 Elsevier B.V.
PY - 2018/12/1
Y1 - 2018/12/1
N2 - Polyunsaturated fatty acids (PUFAs) have key biological roles in fish cells. We recently showed that the phospholipid composition of rainbow trout liver cells (RTL-W1 cell line) modulates their tolerance to an acute cadmium (Cd) challenge. Here, we investigated (i) the extent to which PUFAs and Cd impact fatty acid homeostasis and metabolism in these cells and (ii) possible mechanisms by which specific PUFAs may confer cytoprotection against Cd. First, RTL-W1 cells were cultivated for one week in growth media spiked with 50 μmol L
−1 of either alpha-linolenic acid (ALA, 18:3n-3), eicosapentaenoic acid (EPA, 20:5n-3), linoleic acid (LA, 18:2n-6) or arachidonic acid (AA, 20:4n-6) in order to modulate their fatty acid profile. Then, the cells were challenged with Cd (0, 50 or 100 μmol L
−1) for 24 h prior to assaying viability, fatty acid profile, intracellular Cd content, proteomic landscape and expression levels of genes involved in fatty acid metabolism, synthesis of PUFA-derived signalling molecules and stress response. We observed that the fatty acid supply and, to a lesser extent, the exposure to Cd influenced cellular fatty acid homeostasis and metabolism. The cellular fatty acid composition of fish liver cells modulated their tolerance to an acute Cd challenge. Enrichments in ALA, EPA, and, to a lesser extent, AA conferred cytoprotection while enrichment in LA had no impact on cell viability. The present study ruled out the possibility that cytoprotection reflects a decreased Cd burden. Our results rather suggest that the PUFA-derived cytoprotection against Cd occurs through a reduction of the oxidative stress induced by Cd and a differential induction of the eicosanoid cascade, with a possible role of peroxiredoxin and glutaredoxin (antioxidant enzymes) as well as cytosolic phospholipase A2 (enzyme initiating the eicosanoid cascade).
AB - Polyunsaturated fatty acids (PUFAs) have key biological roles in fish cells. We recently showed that the phospholipid composition of rainbow trout liver cells (RTL-W1 cell line) modulates their tolerance to an acute cadmium (Cd) challenge. Here, we investigated (i) the extent to which PUFAs and Cd impact fatty acid homeostasis and metabolism in these cells and (ii) possible mechanisms by which specific PUFAs may confer cytoprotection against Cd. First, RTL-W1 cells were cultivated for one week in growth media spiked with 50 μmol L
−1 of either alpha-linolenic acid (ALA, 18:3n-3), eicosapentaenoic acid (EPA, 20:5n-3), linoleic acid (LA, 18:2n-6) or arachidonic acid (AA, 20:4n-6) in order to modulate their fatty acid profile. Then, the cells were challenged with Cd (0, 50 or 100 μmol L
−1) for 24 h prior to assaying viability, fatty acid profile, intracellular Cd content, proteomic landscape and expression levels of genes involved in fatty acid metabolism, synthesis of PUFA-derived signalling molecules and stress response. We observed that the fatty acid supply and, to a lesser extent, the exposure to Cd influenced cellular fatty acid homeostasis and metabolism. The cellular fatty acid composition of fish liver cells modulated their tolerance to an acute Cd challenge. Enrichments in ALA, EPA, and, to a lesser extent, AA conferred cytoprotection while enrichment in LA had no impact on cell viability. The present study ruled out the possibility that cytoprotection reflects a decreased Cd burden. Our results rather suggest that the PUFA-derived cytoprotection against Cd occurs through a reduction of the oxidative stress induced by Cd and a differential induction of the eicosanoid cascade, with a possible role of peroxiredoxin and glutaredoxin (antioxidant enzymes) as well as cytosolic phospholipase A2 (enzyme initiating the eicosanoid cascade).
KW - Cadmium
KW - Gene expression
KW - Polyunsaturated fatty acids
KW - Proteomics
KW - RTL-W1 cells
UR - http://www.scopus.com/inward/record.url?scp=85055255757&partnerID=8YFLogxK
U2 - 10.1016/j.aquatox.2018.09.005
DO - 10.1016/j.aquatox.2018.09.005
M3 - Article
SN - 0166-445X
VL - 205
JO - Aquatic toxicology (Amsterdam, Netherlands)
JF - Aquatic toxicology (Amsterdam, Netherlands)
ER -