Abstract
Two-dimensional remains one of the main experimental approaches in proteome analysis. However, comigration of protein leads to several limitations: lack of accuracy in protein identification, impaired comparative quantification, and PTM detection. We have optimized a third additional step of in-gel separation to alleviate comigration associated drawbacks. Spot resolution is strikingly improved following this simple and rapid method and the positive impact on protein and peptide identification from MS/MS data, on the analysis of relative changes in protein abundance, and on the detection of PTM is described.
Original language | English |
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Pages (from-to) | 2077-2082 |
Number of pages | 6 |
Journal | Proteomics |
Volume | 13 |
Issue number | 14 |
DOIs | |
Publication status | Published - 1 Jul 2013 |
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Renard, P. (Manager)
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