Evaluation of three-dimensional gel electrophoresis to improve quantitative profiling of complex proteomes

B. Colignon, M. Raes, M. Dieu, E. Delaive, S. Mauro

Research output: Contribution to journalArticle

Abstract

Two-dimensional remains one of the main experimental approaches in proteome analysis. However, comigration of protein leads to several limitations: lack of accuracy in protein identification, impaired comparative quantification, and PTM detection. We have optimized a third additional step of in-gel separation to alleviate comigration associated drawbacks. Spot resolution is strikingly improved following this simple and rapid method and the positive impact on protein and peptide identification from MS/MS data, on the analysis of relative changes in protein abundance, and on the detection of PTM is described.
Original languageEnglish
Pages (from-to)2077-2082
Number of pages6
JournalProteomics
Volume13
Issue number14
DOIs
Publication statusPublished - 1 Jul 2013

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